Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models

Ankit Pandey; Aditi Darade; Sneha Agrawal

doi:10.12991/jrespharm.1717276

Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models

Abstract

This study evaluates the immunomodulatory potential of Syzygium cumini (jamun) seed extracts using safer solvents—ethyl acetate (EASC) and ethanol (ETSC)—addressing toxicity concerns of methanol. Successive solvent extraction and TLC fingerprinting to differentiate the bioactive compounds between the extracts. The extracts were assessed through in vitro assays (DPPH scavenging, NBT reduction, splenocyte proliferation, Candida albicans phagocytosis) and in vivo cyclophosphamide-induced immunosuppression in rats. The ethanolic extract (ETSC) demonstrated superior antioxidant and immunomodulatory activity compared to EASC, enhancing splenocyte proliferation, phagocytic function, neutrophil adhesion, and bone marrow cellularity. Key outcomes included the restoration of hematological parameters and immune organ indices. ETSC showed dose-dependent efficacy by modulating cytokine release and activating immune cells like macrophages and lymphocytes. The study establishes the therapeutic potential of Syzygium cumini seeds in managing oxidative stress and immune dysfunctions, highlighting ETSC as a promising candidate for further exploration.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Ankit Pandey This is me
0009-0009-8850-5436
India

Aditi Darade This is me
0009-0005-8671-7428
India

Sneha Agrawal ^*
0000-0002-6732-4574
India

Publication Date

October 6, 2026

Submission Date

June 11, 2025

Acceptance Date

September 6, 2026

Published in Issue

Year 2026 Volume: 30 Number: 5

DOI

https://doi.org/10.12991/jrespharm.1717276

IZ

https://izlik.org/JA43RR44XE

Cite

RIS / Bibtex

APA

Pandey, A., Darade, A., & Agrawal, S. (2026). Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models. Journal of Research in Pharmacy, 30(5), 1253-1266. https://doi.org/10.12991/jrespharm.1717276

AMA

1.Pandey A, Darade A, Agrawal S. Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models. J. Res. Pharm. 2026;30(5):1253-1266. doi:10.12991/jrespharm.1717276

Chicago

Pandey, Ankit, Aditi Darade, and Sneha Agrawal. 2026. “Immunomodulatory Potential of Syzygium Cumini Seed Extracts: A Comprehensive Evaluation through ın Vitro and ın Vivo Models”. Journal of Research in Pharmacy 30 (5): 1253-66. https://doi.org/10.12991/jrespharm.1717276.

EndNote

Pandey A, Darade A, Agrawal S (October 1, 2026) Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models. Journal of Research in Pharmacy 30 5 1253–1266.

IEEE

[1]A. Pandey, A. Darade, and S. Agrawal, “Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models”, J. Res. Pharm., vol. 30, no. 5, pp. 1253–1266, Oct. 2026, doi: 10.12991/jrespharm.1717276.

ISNAD

Pandey, Ankit - Darade, Aditi - Agrawal, Sneha. “Immunomodulatory Potential of Syzygium Cumini Seed Extracts: A Comprehensive Evaluation through ın Vitro and ın Vivo Models”. Journal of Research in Pharmacy 30/5 (October 1, 2026): 1253-1266. https://doi.org/10.12991/jrespharm.1717276.

JAMA

1.Pandey A, Darade A, Agrawal S. Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models. J. Res. Pharm. 2026;30:1253–1266.

MLA

Pandey, Ankit, et al. “Immunomodulatory Potential of Syzygium Cumini Seed Extracts: A Comprehensive Evaluation through ın Vitro and ın Vivo Models”. Journal of Research in Pharmacy, vol. 30, no. 5, Oct. 2026, pp. 1253-66, doi:10.12991/jrespharm.1717276.

Vancouver

1.Ankit Pandey, Aditi Darade, Sneha Agrawal. Immunomodulatory potential of Syzygium cumini seed extracts: a comprehensive evaluation through ın vitro and ın vivo models. J. Res. Pharm. 2026 Oct. 1;30(5):1253-66. doi:10.12991/jrespharm.1717276