Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species

Betül Nur Yaşar; Ali Şen; Narin Sadıkoğlu; Duygu Mısırlı; Mahfuz Elmastaş; Leyla Bitiş

doi:10.12991/jrespharm.1719417

Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species

Abstract

Origanum species (oregano), which have been used as spices and medicines since ancient times and are one of the important riches of our country, are also known as important species in terms of the bioactive compounds they contain. In this study, phytochemical contents and biological activities of methanol extracts (OMİN, OSAM and OSOM) obtained from the aerial parts of three endemic Origanum species, Origanum minutiflorum, O. saccatum and O. solymicum, were comparatively analyzed. Extracts were obtained by maceration method. Antioxidant, anti-inflammatory and antidiabetic activities were evaluated using DPPH/ABTS radical, 5-lipoxygenase and α- glucosidase inhibition methods, respectively. Total phenol, flavonoid and triterpene contents were calculated by Folin-Ciocalteu, AlCl3 and Acetic acid/Vanillin/perchloric acid methods, respectively. Phenolic compounds of the active extract were analyzed by HPLC. OSAM with IC50 values of 13.51 µg/mL and 81.23 µg/mL exhibited the strongest antioxidant activity against DPPH and ABTS radicals. OSAM with IC50 value of 84.11 µg/mL and OSOM with IC50 value of 102.00 µg/mL showed the best inhibitory activity against α-glucosidase and 5-lipoxygenase enzymes, respectively. Total phenol and flavonoid content was highest in OSAM (132.30 and 60.32 mg/g) while total triterpene content was highest in OMIN (177.39 mg/g). In the HPLC analysis of the most active OSAM, rosmarinic acid as the major compound was detected in very high amounts (211.061 mg/g extract). These results show that OSAM has a strong antioxidant and antidiabetic activity and that other phenolic acids and flavonoids, especially rosmarinic acid, may be responsible for this activity. However, in vivo studies are needed to be sure of the activities and bioactivity-directed fractionation method is needed to reveal the compounds responsible for the activity.

Keywords

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References

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Details

Primary Language

English

Subjects

Pharmacognosy, Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Betül Nur Yaşar
0009-0005-1176-713X
Türkiye

Ali Şen
0000-0002-2144-5741
Türkiye

Narin Sadıkoğlu
0000-0002-8806-2905
Türkiye

Duygu Mısırlı
0000-0002-3581-4368
Türkiye

Mahfuz Elmastaş
0000-0002-7149-7427
Türkiye

Leyla Bitiş ^*
0000-0003-1167-6666
Türkiye

Publication Date

May 11, 2026

Submission Date

June 13, 2025

Acceptance Date

July 7, 2025

Published in Issue

Year 2026 Volume: 30 Number: 3

DOI

https://doi.org/10.12991/jrespharm.1719417

IZ

https://izlik.org/JA77GJ89FG

Cite

RIS / Bibtex

APA

Yaşar, B. N., Şen, A., Sadıkoğlu, N., Mısırlı, D., Elmastaş, M., & Bitiş, L. (2026). Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species. Journal of Research in Pharmacy, 30(3), 762-772. https://doi.org/10.12991/jrespharm.1719417

AMA

1.Yaşar BN, Şen A, Sadıkoğlu N, Mısırlı D, Elmastaş M, Bitiş L. Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species. J. Res. Pharm. 2026;30(3):762-772. doi:10.12991/jrespharm.1719417

Chicago

Yaşar, Betül Nur, Ali Şen, Narin Sadıkoğlu, Duygu Mısırlı, Mahfuz Elmastaş, and Leyla Bitiş. 2026. “Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum Species”. Journal of Research in Pharmacy 30 (3): 762-72. https://doi.org/10.12991/jrespharm.1719417.

EndNote

Yaşar BN, Şen A, Sadıkoğlu N, Mısırlı D, Elmastaş M, Bitiş L (May 1, 2026) Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species. Journal of Research in Pharmacy 30 3 762–772.

IEEE

[1]B. N. Yaşar, A. Şen, N. Sadıkoğlu, D. Mısırlı, M. Elmastaş, and L. Bitiş, “Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species”, J. Res. Pharm., vol. 30, no. 3, pp. 762–772, May 2026, doi: 10.12991/jrespharm.1719417.

ISNAD

Yaşar, Betül Nur - Şen, Ali - Sadıkoğlu, Narin - Mısırlı, Duygu - Elmastaş, Mahfuz - Bitiş, Leyla. “Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum Species”. Journal of Research in Pharmacy 30/3 (May 1, 2026): 762-772. https://doi.org/10.12991/jrespharm.1719417.

JAMA

1.Yaşar BN, Şen A, Sadıkoğlu N, Mısırlı D, Elmastaş M, Bitiş L. Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species. J. Res. Pharm. 2026;30:762–772.

MLA

Yaşar, Betül Nur, et al. “Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum Species”. Journal of Research in Pharmacy, vol. 30, no. 3, May 2026, pp. 762-7, doi:10.12991/jrespharm.1719417.

Vancouver

1.Betül Nur Yaşar, Ali Şen, Narin Sadıkoğlu, Duygu Mısırlı, Mahfuz Elmastaş, Leyla Bitiş. Phytochemical Analysis and In Vitro Biological Activities of Three Endemic Origanum species. J. Res. Pharm. 2026 May 1;30(3):762-7. doi:10.12991/jrespharm.1719417