Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study

Surekha Rathod; Palak Kasliwal; Pranjali Bawankar; Avinash Kharat

doi:10.12991/jrespharm.1797786

Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study

Abstract

Syzygium cumini (S. cumini) is noted for its pharmacological benefits, including its roles in diabetes management, antioxidant activity, and anti-inflammatory effects. This study aimed to explore the osteogenic potential of S. cumini seed extract by assessing its ability to induce differentiation in human dental pulp stem cells (hDPSCs). hDPSCs were isolated from healthy premolar teeth, characterized by surface marker expression, and subjected to various assays, including mineralization, alkaline phosphatase activity, and gene expression analysis. The MTT assay was used to evaluate cytotoxicity. The findings revealed that hDPSCs expressed CD73, CD90, and CD105, but not CD34, CD45, or HLA-DR. S. cumini seed extract at 20 μg/ml enhanced hDPSCs viability, proliferation, and osteogenic matrix production without cytotoxic effects. Staining with 2% Alizarin Red S confirmed osteocyte differentiation, and the extract significantly boosted gene expression related to osteogenesis. These results suggest that S. cumini seed extract effectively promotes osteogenic differentiation in hDPSCs, highlighting its potential for bone regeneration and warranting further research into its therapeutic applications.

Keywords

References

[1] Tang H, Hosein A, Mattioli-Belmonte M. Traditional Chinese Medicine and orthopedic biomaterials: Host of opportunities from herbal extracts. Mater Sci Eng C Mater Biol Appl. 2021;120:111760. https://doi.org/10.1016/j.msec.2020.111760
[2] Yuan H, Ma Q, Ye L, Piao G. The Traditional medicine and modern medicine from natural products. Molecules. 2016;21(5):559. https://doi.org/10.3390/molecules21050559
[3] Rizvi MK, Rabail R, Munir S, et al. Astounding Health Benefits of Jamun (Syzygium cumini) toward Metabolic Syndrome. Molecules. 2022;27(21):7184. http://doi.org/10.3390/molecules27217184
[4] Rizvi SI, Mishra N. Traditional Indian medicines used for the management of diabetes mellitus. J Diabetes Res. 2013;2013:712092. http://doi.org/10.1155/2013/712092
[5] Chaudhuri AN, Pal S, Gomes A, Bhattacharya S. Anti‐inflammatory and related actions of Syzygium cuminii seed extract. Phytother Res. 1990;4(1):5-10. https://doi.org/10.1002/ptr.2650040103
[6] Mahapatra PK, Chakraborty D, Chaudhuri AK. Anti-Inflammatory and antipyretic activities of Syzygium cuminii. Planta Med. 1986;(6):540. http://doi.org/10.1055/s-2007-969339
[7] Banerjee J, Narendhirakannan RT. Phytochemical analyses, antibacterial, in vitro antioxidant and cytotoxic activities of ethanolic extract of Syzygium cumini (L.) seed extract. Int J Pharm Sci Res. 2011;2(7):1799-1806. https://doi.org/10.5555/20113340230
[8] Sharma SB, Nasir A, Prabhu KM, Murthy PS, Dev G. Hypoglycaemic and hypolipidemic effect of ethanolic extract of seeds of Eugenia jambolana in alloxan-induced diabetic rabbits. J Ethnopharmacol. 2003;85(2-3):201-206. http://doi.org/10.1016/s0378-8741(02)00366-5

[9] Ribeiro RM, Pinheiro Neto VF, Ribeiro KS, Vieira DA, Abreu IC, Silva SD, Cartágenes MD, Freire SM, Borges AC, Borges MO. Antihypertensive effect of Syzygium cumini in spontaneously hypertensive rats. Evid Based Complement Alternat Med. 2014;2014(1):605452. http://doi.org/10.1155/2014/605452
[10] Singh H. Recent development of novel drug delivery of herbal drugs. RPS Pharmacy Pharmacol Rep. 2023;2(4):rqad028. https://doi.org/10.1093/rpsppr/rqad028
[11] Das A, Yavagal PC, Reddy D. Antibacterial Efficacy of E. Jambolana (Syzygium cumini) seeds extracts against periodontal pathogens: An in-vitro study. Int J Ayurveda Res. 2021;20:16-20. https://doi.org/10.47070/ijapr.v9i10.2051
[12] Agriesti F, Landini F, Tamma M, Pacelli C, Mazzoccoli C, Calice G, Ruggieri V, Capitanio G, Mori G, Piccoli C, Capitanio N. Bioenergetic profile and redox tone modulate in vitro osteogenesis of human dental pulp stem cells: new perspectives for bone regeneration and repair. Stem Cell Res Ther. 2023;14(1):215. http:/doi.org/10.1186/s13287-023-03447-9
[13] Anitua E, Troya M, Zalduendo M. Progress in the use of dental pulp stem cells in regenerative medicine. Cytotherapy. 2018;20(4):479-498. http://doi.org/10.1016/j.jcyt.2017.12.011
[14] Ayyanar M, Subash-Babu P. Syzygium cumini (L.) Skeels: a review of its phytochemical constituents and traditional uses. Asian Pac J Trop Biomed. 2012;2(3):240-246. http://doi.org/10.1016/S2221-1691(12)60050-1
[15] Kumar EK, Mastan SK, Reddy KR, Reddy GA, Raghunandan N, Chaitanya G. Anti-arthritic property of the methanolic extract of Syzygium cumini seeds. Int J Integr Biol. 2008;4(1):55-61.
[16] Patil VR, Kharat AH, Kulkarni DG, Kheur SM, Bhonde RR. Long term explant culture for harvesting homogeneous population of human dental pulp stem cells. Cell Biol Int. 2018;42(12):1602-1610. https://doi.org/10.1002/cbin.11065
[17] Lin CY, Tsai MS, Kuo PJ, Chin YT, Weng IT, Wu Y, Huang HM, Hsiung CN, Lin HY, Lee SY. 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucoside promotes the effects of dental pulp stem cells on rebuilding periodontal tissues in experimental periodontal defects. J Periodontol. 2021;92(2):306-316. http://doi.org/10.1002/JPER.19-0563
[18] Kharat A, Nagar A, Sanap A, Sakhare S, Kheur S, Dubewar A, Bhonde R. Xanthium strumarium seed extract boosts osteogenesis in human dental pulp stem cell model. J Ayurveda Integr Med. 2023;14(6):100811. http://doi.org/10.1016/j.jaim.2023.100811
[19] Lafzi A, Saravi NSV, Amid R, Kadkhodazadeh M, Shojaei N. Biological reactions of dental pulp stem cells cultured in presence of new xenograft bone substitutes from different sources: An in vitro study. J Indian Soc Periodontol. 2022;26(5):440-445. http://doi.org/10.4103/jisp.jisp_739_20

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Surekha Rathod ^*
0000-0001-9137-120X
India

Palak Kasliwal
0009-0001-4751-2762
India

Pranjali Bawankar This is me
0000-0002-7271-5676
India

Avinash Kharat This is me
0000-0002-5647-8340
India

Publication Date

November 2, 2025

Submission Date

August 24, 2024

Acceptance Date

December 14, 2024

Published in Issue

Year 2025 Volume: 29 Number: 6

DOI

https://doi.org/10.12991/jrespharm.1797786

IZ

https://izlik.org/JA57UX64SB

Cite

RIS / Bibtex

APA

Rathod, S., Kasliwal, P., Bawankar, P., & Kharat, A. (2025). Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study. Journal of Research in Pharmacy, 29(6), 2308-2316. https://doi.org/10.12991/jrespharm.1797786

AMA

1.Rathod S, Kasliwal P, Bawankar P, Kharat A. Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study. J. Res. Pharm. 2025;29(6):2308-2316. doi:10.12991/jrespharm.1797786

Chicago

Rathod, Surekha, Palak Kasliwal, Pranjali Bawankar, and Avinash Kharat. 2025. “Evaluation of Osteogenic Potential of Syzygium Cumini Seed Extract on Human Dental Pulp Stem Cells: An in Vitro Study”. Journal of Research in Pharmacy 29 (6): 2308-16. https://doi.org/10.12991/jrespharm.1797786.

EndNote

Rathod S, Kasliwal P, Bawankar P, Kharat A (November 1, 2025) Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study. Journal of Research in Pharmacy 29 6 2308–2316.

IEEE

[1]S. Rathod, P. Kasliwal, P. Bawankar, and A. Kharat, “Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study”, J. Res. Pharm., vol. 29, no. 6, pp. 2308–2316, Nov. 2025, doi: 10.12991/jrespharm.1797786.

ISNAD

Rathod, Surekha - Kasliwal, Palak - Bawankar, Pranjali - Kharat, Avinash. “Evaluation of Osteogenic Potential of Syzygium Cumini Seed Extract on Human Dental Pulp Stem Cells: An in Vitro Study”. Journal of Research in Pharmacy 29/6 (November 1, 2025): 2308-2316. https://doi.org/10.12991/jrespharm.1797786.

JAMA

1.Rathod S, Kasliwal P, Bawankar P, Kharat A. Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study. J. Res. Pharm. 2025;29:2308–2316.

MLA

Rathod, Surekha, et al. “Evaluation of Osteogenic Potential of Syzygium Cumini Seed Extract on Human Dental Pulp Stem Cells: An in Vitro Study”. Journal of Research in Pharmacy, vol. 29, no. 6, Nov. 2025, pp. 2308-16, doi:10.12991/jrespharm.1797786.

Vancouver

1.Surekha Rathod, Palak Kasliwal, Pranjali Bawankar, Avinash Kharat. Evaluation of osteogenic potential of Syzygium cumini seed extract on human dental pulp stem cells: An in vitro study. J. Res. Pharm. 2025 Nov. 1;29(6):2308-16. doi:10.12991/jrespharm.1797786