Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs

Özlem Çoban; Nurseli Saylam; Elif Serenay Baran; Leyla Küçüktemel

doi:10.12991/jrespharm.1797806

Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs

Abstract

Bovine serum albumin (BSA) is a natural protein that can form complexes in various forms. It is widely used in drug delivery due to its low molecular weight and biocompatible, biodegradable, non-toxic and nonimmunological properties. Sertraline (SRN), selected as a model drug in this study, is a selective serotonin reuptake inhibitor and has low water solubility. In current study, a series of SRN-loaded BSA nanoparticles (NPs) were prepared by desolvation/coacervation method using Multilevel Categorical and D-Optimal designs to determine critical formulation parameters (CPPs) (BSA concentration, mixing speed, ethanol:water ratio and ionic strength). 20 different formulations were analyzed for particle size (PS), polydispersity index (PdI), zeta potential (ZP), encapsulation efficiency (EE) and nanoparticle yield (NY). In vitro dissolution studies were performed for selected SRN-loaded BSANPs, and F18 with 537.6±4.5 nm PS, 0.124±0.020 PdI, -36.2±0.1 mV ZP, 102.78±0.02% EE and 96.66±0.01% NY showed a release rate closer to the SRN suspension. Moreover, in TEM analysis, it exhibited spherical and grape cluster structures typical for BSA-NPs. In this context, we concluded that BSA NPs may offer potential applications as nanosystems for SRN.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Özlem Çoban ^*
0000-0003-1154-7602
Türkiye

Nurseli Saylam
0009-0000-5080-2747
Türkiye

Elif Serenay Baran
0009-0006-9199-0581
Türkiye

Leyla Küçüktemel This is me
0009-0008-2809-800X
Türkiye

Publication Date

November 2, 2025

Submission Date

May 15, 2024

Acceptance Date

August 29, 2024

Published in Issue

Year 2025 Volume: 29 Number: 6

DOI

https://doi.org/10.12991/jrespharm.1797806

IZ

https://izlik.org/JA39UN65FD

Cite

RIS / Bibtex

APA

Çoban, Ö., Saylam, N., Baran, E. S., & Küçüktemel, L. (2025). Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs. Journal of Research in Pharmacy, 29(6), 2317-2326. https://doi.org/10.12991/jrespharm.1797806

AMA

1.Çoban Ö, Saylam N, Baran ES, Küçüktemel L. Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs. J. Res. Pharm. 2025;29(6):2317-2326. doi:10.12991/jrespharm.1797806

Chicago

Çoban, Özlem, Nurseli Saylam, Elif Serenay Baran, and Leyla Küçüktemel. 2025. “Preparation and Evaluation of Sertraline-Loaded Bovine Serum Albumin Nanoparticles via Multilevel Categorical and D-Optimal Designs”. Journal of Research in Pharmacy 29 (6): 2317-26. https://doi.org/10.12991/jrespharm.1797806.

EndNote

Çoban Ö, Saylam N, Baran ES, Küçüktemel L (November 1, 2025) Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs. Journal of Research in Pharmacy 29 6 2317–2326.

IEEE

[1]Ö. Çoban, N. Saylam, E. S. Baran, and L. Küçüktemel, “Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs”, J. Res. Pharm., vol. 29, no. 6, pp. 2317–2326, Nov. 2025, doi: 10.12991/jrespharm.1797806.

ISNAD

Çoban, Özlem - Saylam, Nurseli - Baran, Elif Serenay - Küçüktemel, Leyla. “Preparation and Evaluation of Sertraline-Loaded Bovine Serum Albumin Nanoparticles via Multilevel Categorical and D-Optimal Designs”. Journal of Research in Pharmacy 29/6 (November 1, 2025): 2317-2326. https://doi.org/10.12991/jrespharm.1797806.

JAMA

1.Çoban Ö, Saylam N, Baran ES, Küçüktemel L. Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs. J. Res. Pharm. 2025;29:2317–2326.

MLA

Çoban, Özlem, et al. “Preparation and Evaluation of Sertraline-Loaded Bovine Serum Albumin Nanoparticles via Multilevel Categorical and D-Optimal Designs”. Journal of Research in Pharmacy, vol. 29, no. 6, Nov. 2025, pp. 2317-26, doi:10.12991/jrespharm.1797806.

Vancouver

1.Özlem Çoban, Nurseli Saylam, Elif Serenay Baran, Leyla Küçüktemel. Preparation and evaluation of sertraline-loaded bovine serum albumin nanoparticles via multilevel categorical and D-optimal designs. J. Res. Pharm. 2025 Nov. 1;29(6):2317-26. doi:10.12991/jrespharm.1797806