Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation

Devang Tandel; Kalpana Patel; Vaishali Thakkar; Tejal Gandhi; Jinal Tandel

doi:10.12991/jrespharm.1798001

Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation

Abstract

A rapid and precise high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been developed for the quantitative determination of Rifampicin and Quercetin, in bronchoalveolar lavage fluid. The validation process was conducted in compliance with the USFDA guidelines. Separation was achieved using a C18-Luna Phenomenex column (5 μm, 4.60 × 150 mm) with a mobile phase comprising 20 mM disodium hydrogen orthophosphate (pH 4.5) and methanol in a 35:65 %v/v ratio. The retention times for Quercetin and Rifampicin were 5.42 and 8.60 minutes, respectively. Optimal protein precipitation was achieved using 500 μl of acetonitrile and 500 μl of methanol, yielding an extraction recovery exceeding 80 % for both drugs from bronchoalveolar lavage fluid. The extraction method yielded clean samples, rendering the process both straightforward and cost-effective. The method exhibited a strong linear correlation (r > 0.9995) between the peak area ratio and the concentrations for Rifampicin (50– 5000 ng/ml) and Quercetin (40–4000 ng/ml). Pharmacokinetic analysis revealed that poly(lactic-co-glycolic acid) nanoparticles sustained higher drug concentrations in the lung tissue for an extended period of up to 24 hours. In conclusion, the developed HPLC method, utilizing simple UV detection, offers several advantages, including costeffectiveness, high recovery rates, a broad linear range, robust quantification capability, and short analysis times.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Devang Tandel ^*
0000-0001-7013-1514
India

Kalpana Patel
0000-0003-1659-6307
India

Vaishali Thakkar This is me
0000-0001-6332-7703
India

Tejal Gandhi
0000-0002-7927-0331
India

Jinal Tandel
0000-0003-1669-6519
India

Publication Date

November 2, 2025

Submission Date

September 11, 2024

Acceptance Date

November 9, 2024

Published in Issue

Year 2025 Volume: 29 Number: 6

DOI

https://doi.org/10.12991/jrespharm.1798001

IZ

https://izlik.org/JA96MA55UB

Cite

RIS / Bibtex

APA

Tandel, D., Patel, K., Thakkar, V., Gandhi, T., & Tandel, J. (2025). Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation. Journal of Research in Pharmacy, 29(6), 2508-2518. https://doi.org/10.12991/jrespharm.1798001

AMA

1.Tandel D, Patel K, Thakkar V, Gandhi T, Tandel J. Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation. J. Res. Pharm. 2025;29(6):2508-2518. doi:10.12991/jrespharm.1798001

Chicago

Tandel, Devang, Kalpana Patel, Vaishali Thakkar, Tejal Gandhi, and Jinal Tandel. 2025. “Simultaneous Quantification of Quercetin and Rifampicin in Bronchoalveolar Lavage Fluid by HPLC-UV Technique and Its Application to a Pharmacokinetic Study of Investigational Dry Powder Formulation”. Journal of Research in Pharmacy 29 (6): 2508-18. https://doi.org/10.12991/jrespharm.1798001.

EndNote

Tandel D, Patel K, Thakkar V, Gandhi T, Tandel J (November 1, 2025) Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation. Journal of Research in Pharmacy 29 6 2508–2518.

IEEE

[1]D. Tandel, K. Patel, V. Thakkar, T. Gandhi, and J. Tandel, “Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation”, J. Res. Pharm., vol. 29, no. 6, pp. 2508–2518, Nov. 2025, doi: 10.12991/jrespharm.1798001.

ISNAD

Tandel, Devang - Patel, Kalpana - Thakkar, Vaishali - Gandhi, Tejal - Tandel, Jinal. “Simultaneous Quantification of Quercetin and Rifampicin in Bronchoalveolar Lavage Fluid by HPLC-UV Technique and Its Application to a Pharmacokinetic Study of Investigational Dry Powder Formulation”. Journal of Research in Pharmacy 29/6 (November 1, 2025): 2508-2518. https://doi.org/10.12991/jrespharm.1798001.

JAMA

1.Tandel D, Patel K, Thakkar V, Gandhi T, Tandel J. Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation. J. Res. Pharm. 2025;29:2508–2518.

MLA

Tandel, Devang, et al. “Simultaneous Quantification of Quercetin and Rifampicin in Bronchoalveolar Lavage Fluid by HPLC-UV Technique and Its Application to a Pharmacokinetic Study of Investigational Dry Powder Formulation”. Journal of Research in Pharmacy, vol. 29, no. 6, Nov. 2025, pp. 2508-1, doi:10.12991/jrespharm.1798001.

Vancouver

1.Devang Tandel, Kalpana Patel, Vaishali Thakkar, Tejal Gandhi, Jinal Tandel. Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation. J. Res. Pharm. 2025 Nov. 1;29(6):2508-1. doi:10.12991/jrespharm.1798001