Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell

Moghe Tarini; Rasane Sheetal; Rhonde Ramesh; Mante Nishant; Potdar Pranjali; Sakhare Swapnali; Sanap Avinash; Avinash Kharat

doi:10.12991/jrespharm.1798400

Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell

Abstract

Herbal medicine, a centuries-old practice, has gained increasing attention in the modern healthcare landscape. Yashtimadhu is an ancient herbal medicine which has anti-inflammatory, anti-microbial and wound healing properties. It contains phytochemical component such as glabridin, glycyrrhizin, licochalcone which are responsible for wound healing. Our objective is to study the role of Yashtimadhu aqueous extracts (YAC) on dental pulp stem cells (DPSCs) with respect to its wound healing action. DPSCs were isolated and characterized for surface marker CD34, CD45, CD73, CD90, CD105 and HLA-DR. The cytotoxicity of Yashtimadhu extract was studied using MTT assay at various concentrations like 2, 5, 10, 15, 20, 25, 50 μg/ml. Later, scratch assay was performed. Cells were treated with Mitomycin C to inhibit cell division and further treated with aqueous extracts of Yashtimadhu to evaluate its effect on cell migration. Scratch was observed at 6 and 24 hours and images were taken under inverted microscope. Further the image was analyzed using image J software. Cell cycle analysis was performed using flow cytometer. Using Yashtimadhu extract on DPSC cell lines demonstrated no cytotoxic effects and increased cell migration, aiding in wound repair. Additionally, combining Yashtimadhu with Mitomycin C enhanced wound healing and cell migration, suggesting its potential for novel tissue repair therapies.

Keywords

References

Tanaka A, Horiuchi M, Umano K, Shibamoto T. Antioxidant and anti-inflammatory activities of water distillate and its dichloromethane extract from licorice root (Glycyrrhiza uralensis) and chemical composition of dichloromethane extract. J Sci Food Agric. 2008; 88(7): 1158-1165. https://doi.org/10.1002/JSFA.3191
Menegazzi M, Paola R, Mazzon E, Genovese T, Crisafulli C, Bosco M, Zou Z, Suzuki H, Cuzzocrea S. Glycyrrhizin attenuates the development of carrageenan-induced lung injury in mice. Pharmacol Res. 2008; 58(1): 22-31. https://doi.org/10.1016/J.PHRS.2008.05.012
Sharma H, Yunus G, Mohapatra A, Kulshrestha R, Agrawal R, Kalra M. Antimicrobial efficacy of three medicinal plants Glycyrrhiza glabra, Ficus religiosa, and Plantago major on inhibiting primary plaque colonizers and periodontal pathogens: An in vitro study. Ind J Dent Res. 2016; 27(2): 200-204. https://doi.org/10.4103/0970- 9290.183135
Najeeb V, Al-Refai A. Antibacterial effect and healing potential of topically applied licorice root extract on experimentally induced oral wounds in rabbits. Saudi J Oral Sci. 2015; 2(1): 10-13. https://doi.org/10.4103/1658- 6816.150583
Mermariani Z, Hajimahmoodi M, Minaee B, Khodagholi F, Yans A, Rahimi R, Amin G, Moghaddam G, Toliyat T, Sharifzadeh M. Protective effect of a polyherbal traditional formula consisting of Rosa damascena Mill., Glycyrrhiza glabra L. And Nardostachys jatamansi DC., against ethanol-induced gastric ulcer. Iran J Pharm Res. 2017; 16(2): 694– 707. https://pubmed.ncbi.nlm.nih.gov/28979324/
Aisewariya A, Reddy V. Effect of Yashtimadhu suppository with cocoa butter base in the pain management of parikartika. Int J Ayurveda Pharm Res. 2022; 10(11): 73–80. https://doi.org/10.47070/ijapr.v10i11.2596
Patel J, Dudhamal T. A comparative clinical study of Yashtimadhu ghrita and lignocaine-nifedipine ointment in the management of parikartika (acute fissure-in-ano). Ayu.2017; 38(1-2): 46-51. https://doi.org/10.4103/AYU.AYU_93_17
Patil A, Kasat P, Gundre R. The effect of Yashtimadhu ointment on sadyovrana - an experimental study. Nat J ResAyurveda Sci. 2020; 8(04): 1–4. https://doi.org/10.52482/AYURLOG.V8I04.646

Despande R, Devane Y, Patil L. Role of Yeshtimadhu [Glycyrrhiza glabra linn] in cervical erosion. World J Pharm Med Res. 2015; 8(7): 104-106. https://doi.org/10.1002/ptrr.2693
Jung J, Lee Y, Kim S, Kim K, Kim K, Oh S, Jung Y. Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease. BMC Complement Altern Med. 2016;16:19. https://doi.org/10.1186/s12906-016-0997-0
Sharma V, Katiyar A, Agrawal R. Glycyrrhiza glabra: chemistry and pharmacological activity. Sweetners. 2018; 87– 100. https://doi.org/10.1007/978-3-319-27027-2_21
Muslikh F, Priatna P, Ekasari W. Pharmacological effects of Glycrrhiza glabra L. as antihepatitis and hepatoprotective for children. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia. 2023; 10(1): 126-140. https://doi.org/10.20473/jfiki.v10i12023
Thakur A, Raj P. Pharmacological perspective of Glycyrrhiza glabra Linn.: a mini-review. J Anal Pharm Res. 2017; 5(5): 1-5. https://doi.org/10.15406/japlr.2017.05.00156
Chen X, Fang D, Li L, Chen L, Li Q, Gong F, Fang M. Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells. Immunol Res. 2017; 65(3): 666-680. https://doi.org/10.1007/S12026-017-8894-2
Mees M, Boone F, Bouwen T, Vanaerschot F, Titeca C, Vikkula H, Catrysse L, Vananroye A, Koos E, Alexandris S, Rosenfeldt S, Eyley S, Koetz J, Loo G, Thielemans W, Hoste E. Glycyrrhizin-based hydrogels accelerate wound healing of normoglycemic and diabetic mouse skin. Pharmaceutics. 2023; 15(1): 27. https://doi.org/10.3390/pharmaceutics15010027
Guo S, DiPietro L. Factors affecting wound healing. J Dent Res. 2010; 89(3): 219-229. https://doi.org/10.1177/0022034509359125
Kotian S, Bhat K, Pai S, Nayak J, Souza A, Gourisheti K, Padma D. The role of natural medicines on wound healing: A biomechanical, histological, biochemical and molecular study. Ethiop J Health Sci. 2018; 28(6): 759-770. https://doi.org/10.4314/ejhs.v28i6.11
Assar D, Elhabashi N, Mokhbatly A, Ragab A, Elbialy Z, Rizk S, Albalawi A, Althobaiti N, Jaouni S, Atiba A. Wound healing potential of licorice extract in rat model: Antioxidants, histopathological, immunohistochemical and gene expression evidences. Biomed Pharmacother. 2021; 143: 112151. https://doi.org/10.1016/J.BIOPHA.2021.112151
Udalamaththa V, Jayasinghe C, Udagama P. Potential role of herbal remedies in stem cell therapy: proliferation and differentiation of human mesenchymal stromal cells. Stem Cell Res Ther. 2016; 7: 110. https://doi.org/10.1186/s13287-016-0366-4
Wahab S, Annadurai S, Abullais S, Das G, Ahmad W, Ahmad M, Kandasamy G, Vasudevan R, Ali M, Amir M. Glycyrrhiza glabra (licorice): A comprehensive review on its phytochemistry, biological activities, clinical evidence and toxicology. Plants. 2021; 10: 2751. https://doi.org/10.3390/plants10122751
Tu C, Cunningham N, Zhang M, Wu J. Human induced pluripotent stem cells as a screening platform for drug- induced vascular toxicity. Front Pharmacol. 2021; 12:613837. https://doi.org/10.3389/FPHAR.2021.613837/BIBTEX
Chang E, Jin S, Nam M, Kim S. Human induced pluripotent stem cells: clinical significance and applications in neurologic diseases. J Korean Neurosurg Soc. 2019; 62(5): 493-501. https://doi.org/10.3340/JKNS.2018.0222
Oh E, Lee H, Kalimuthu S, Kim T, Kim H, Baek S, Zhu L, Oh J, Son S, Chung H, Ahn B. In vivo migration of mesenchymal stem cells to burn injury sites and their therapeutic effects in a living mouse model. J Control Release. 2018; 279: 79-88. https://doi.org/10.1016/J.JCONREL.2018.04.020
Francois S, Mouiseddine M, Allenet-Lepage B, Voswinkel J, Douay L, Benderitter M, Chapel A. Human mesenchymal stem cells provide protection against radiation-induced liver injury by antioxidative process, vasculature protection, hepatocyte differentiation, and trophic effects. BioMed Res Int. 2013; 2013: 151679. https://doi.org/10.1155/2013/151679
Zhang Q, Lin L, Ye W. Techniques for extraction and isolation of natural products: A comprehensive review. Chin Med (United Kingdom). 2018; 13(20): 1-26. https://doi.org/10.1186/s13020-018-0177-x
Rao V, Nadig R, Nadig R, Karthik J, Pai V, Rao. Isolation and characterization of human dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED)-an in vitro study. RGUHS J Dent Sci. 2018; 10(2): 9-18. https://journalgrid.com/view/article/rjds/194
Garg N, Jain A. Therapeutic and medicinal uses of Yashtimadhu: A review. Int J Sci Res. 2022; 11(2): 1-2. https://doi.org/10.36106/ijsr
Wells A, Nuschke A, Yates C. Skin tissue repair: matrix microenvironmental influences. Matrix Biol. 2016; 49: 25-36. https://doi.org/10.1016/J.MATBIO.2015.08.001
Ghabaee D, Ebrahimzadeh M, Akbari J, Amiri F. Wound healing activity of Sambucus ebulus. Int J Pharm Sci Res. 2017; 8(1): 132-135. https://doi.org/10.13040/IJPSR.0975-8232.8(1).132-35
Azizsoltani A, Piri K, Behzad S, Soleimani M, Nekouei M, Mahmoudi Z, Kazemi A. Ethyl acetate extract of licorice root (Glycyrrhiza glabra) enhances proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells. Iran J Pharm Res. 2018; 17(3): 1057-1067.
Mouritzen M, Jenssen H. Optimized scratch assay for in vitro testing of cell migration with an automated optical camera. J Vis Exp. 2018; 138:57691. https://doi.org/10.3791/57691
Dhillon P, Li X, Sanes J, Akintola O, Sun B. Method comparison for analyzing wound healing rates. Biochem Cell Biol. 2017; 95(3): 450-454. https://doi.org/10.1139/BCB-2016-0163

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Moghe Tarini This is me
0009-0001-9340-5706
India

Rasane Sheetal This is me
0000-0003-1356-9864
India

Rhonde Ramesh This is me
0000-0001-9955-2056
India

Mante Nishant This is me
0000-0002-2293-8345
India

Potdar Pranjali This is me
0009-0001-1697-7153
India

Sakhare Swapnali This is me
0000-0002-5064-7182
India

Sanap Avinash This is me
0000-0003-2535-0958
India

Avinash Kharat ^*
0000-0002-5647-8340
India

Publication Date

November 2, 2025

Submission Date

July 13, 2024

Acceptance Date

December 14, 2024

Published in Issue

Year 2025 Volume: 29 Number: 6

DOI

https://doi.org/10.12991/jrespharm.1798400

IZ

https://izlik.org/JA87KT63MD

Cite

RIS / Bibtex

APA

Tarini, M., Sheetal, R., Ramesh, R., Nishant, M., Pranjali, P., Swapnali, S., Avinash, S., & Kharat, A. (2025). Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell. Journal of Research in Pharmacy, 29(6), 2602-2609. https://doi.org/10.12991/jrespharm.1798400

AMA

1.Tarini M, Sheetal R, Ramesh R, et al. Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell. J. Res. Pharm. 2025;29(6):2602-2609. doi:10.12991/jrespharm.1798400

Chicago

Tarini, Moghe, Rasane Sheetal, Rhonde Ramesh, et al. 2025. “Investigating the Healing Potential of Yashtimadhu (licorice) Using Dental Pulp Stem Cell”. Journal of Research in Pharmacy 29 (6): 2602-9. https://doi.org/10.12991/jrespharm.1798400.

EndNote

Tarini M, Sheetal R, Ramesh R, Nishant M, Pranjali P, Swapnali S, Avinash S, Kharat A (November 1, 2025) Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell. Journal of Research in Pharmacy 29 6 2602–2609.

IEEE

[1]M. Tarini et al., “Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell”, J. Res. Pharm., vol. 29, no. 6, pp. 2602–2609, Nov. 2025, doi: 10.12991/jrespharm.1798400.

ISNAD

Tarini, Moghe - Sheetal, Rasane - Ramesh, Rhonde - Nishant, Mante - Pranjali, Potdar - Swapnali, Sakhare - Avinash, Sanap - Kharat, Avinash. “Investigating the Healing Potential of Yashtimadhu (licorice) Using Dental Pulp Stem Cell”. Journal of Research in Pharmacy 29/6 (November 1, 2025): 2602-2609. https://doi.org/10.12991/jrespharm.1798400.

JAMA

1.Tarini M, Sheetal R, Ramesh R, Nishant M, Pranjali P, Swapnali S, Avinash S, Kharat A. Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell. J. Res. Pharm. 2025;29:2602–2609.

MLA

Tarini, Moghe, et al. “Investigating the Healing Potential of Yashtimadhu (licorice) Using Dental Pulp Stem Cell”. Journal of Research in Pharmacy, vol. 29, no. 6, Nov. 2025, pp. 2602-9, doi:10.12991/jrespharm.1798400.

Vancouver

1.Moghe Tarini, Rasane Sheetal, Rhonde Ramesh, Mante Nishant, Potdar Pranjali, Sakhare Swapnali, Sanap Avinash, Avinash Kharat. Investigating the healing potential of yashtimadhu (licorice) using dental pulp stem cell. J. Res. Pharm. 2025 Nov. 1;29(6):2602-9. doi:10.12991/jrespharm.1798400