Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities

Thoriqul Hıdayah; Risky Hadı Wıbowo; Abdi Wira Septama; Sipriyadi Sipriyadi; Morina Adfa; Muhammad Eka Prastya; Eddy Sukmawinata; Muskhazli Mustafa; Eliza Farestiani

doi:10.12991/jrespharm.1798454

Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities

Abstract

Xylocarpus granatum is a true mangrove species that belongs to Meliaceae family. X.granatum serves limonoids compounds commonly utilized by coastal communities as traditional medicine. This study is designed to investigate antioxidant, antibacterial, and antibiofilm activities of bioactive compounds produced by associated endophytic bacteria isolated from X.granatum leaves. The endophytic bacteria isolate XGDE 7 was obtained from the collection of microbiology laboratory, Department of Biology, University of Bengkulu. Based on 16sRNA analysis, this isolate is closely related to P.aeruginosa strain BSP12 with similarity of 99.82 %. The antibacterial activity using pellet and supernatant exhibited that the widest inhibition zone diameters were found in inhibiting Bacillus subtilis (7.93 ± 0.42 mm; 3.70 ± 0.16 mm ). Furthermore, the largest clear zone using crude extract was shown against Escherhicia.coli (12.29 ± 0.50 mm). The lowest MIC of XGDE 7 was found on Eenterococcus faecalis (15.62 µg/ml) and the highest MIC was on Stapylococcus aures (62.50 µg/ml). As for MBC, XGDE 7 isolate showed the lowest activity on E.faecalis (31.25 µg/ml), while the highest was observed on MBC S. aureus (125 µg/ml). Antibiofilm activity of XGDE 7 extract S.aureus, B.subtilis, E.coli and E.faecalis effectively inhibited at a concentration range of 2 x MIC with inhibition range of 80.10±0.5 to 74.79 ± 0.1%. Meanwhile, the antioxidant activity using DPPH method exhibited lC50 of 49.25 µg/ml. GC-MS analysis revealed various bioactive compounds; Cyclo (L-prolyl-L-valine), Hexadecanoic acid, methyl ester, Pyrrolo[1,2-a]pyrazine-1,4- dione, hexahydro-3-(2-methylpropyl), 9-octadecenoic acid (z)-,methyl ester, 10-Octadecenoic acid,methyl ester, 2,5- Piperazinedione, 3,6-bis (2-methylpropyl), and Pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3(phenylmethyl).

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Thoriqul Hıdayah
0009-0005-2719-3046
Indonesia

Risky Hadı Wıbowo ^*
0000-0002-5917-4625
Indonesia

Abdi Wira Septama
0000-0002-6471-7735
Indonesia

Sipriyadi Sipriyadi
0000-0003-1042-2576
Indonesia

Morina Adfa
0000-0002-7944-3992
Indonesia

Muhammad Eka Prastya This is me
0000-0003-2500-1264
Indonesia

Eddy Sukmawinata
0000-0002-8749-0270
Indonesia

Muskhazli Mustafa This is me
0000-0002-4545-487X
Malaysia

Eliza Farestiani This is me
0009-0000-2460-2231
Indonesia

Publication Date

November 2, 2025

Submission Date

November 14, 2024

Acceptance Date

January 11, 2025

Published in Issue

Year 2025 Volume: 29 Number: 6

DOI

https://doi.org/10.12991/jrespharm.1798454

IZ

https://izlik.org/JA48XG86ZD

Cite

RIS / Bibtex

APA

Hıdayah, T., Hadı Wıbowo, R., Septama, A. W., Sipriyadi, S., Adfa, M., Prastya, M. E., Sukmawinata, E., Mustafa, M., & Farestiani, E. (2025). Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities. Journal of Research in Pharmacy, 29(6), 2633-2642. https://doi.org/10.12991/jrespharm.1798454

AMA

1.Hıdayah T, Hadı Wıbowo R, Septama AW, et al. Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities. J. Res. Pharm. 2025;29(6):2633-2642. doi:10.12991/jrespharm.1798454

Chicago

Hıdayah, Thoriqul, Risky Hadı Wıbowo, Abdi Wira Septama, et al. 2025. “Secondary Metabolites of Endophytic Pseudomonas Aeruginosa XGDE 7 from Mangrove Xylocarpus Granatum J. Koenig : Antibacterial, Antibiofilm, and Antioxidant Activities”. Journal of Research in Pharmacy 29 (6): 2633-42. https://doi.org/10.12991/jrespharm.1798454.

EndNote

Hıdayah T, Hadı Wıbowo R, Septama AW, Sipriyadi S, Adfa M, Prastya ME, Sukmawinata E, Mustafa M, Farestiani E (November 1, 2025) Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities. Journal of Research in Pharmacy 29 6 2633–2642.

IEEE

[1]T. Hıdayah et al., “Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities”, J. Res. Pharm., vol. 29, no. 6, pp. 2633–2642, Nov. 2025, doi: 10.12991/jrespharm.1798454.

ISNAD

Hıdayah, Thoriqul - Hadı Wıbowo, Risky - Septama, Abdi Wira - Sipriyadi, Sipriyadi - Adfa, Morina - Prastya, Muhammad Eka - Sukmawinata, Eddy - Mustafa, Muskhazli - Farestiani, Eliza. “Secondary Metabolites of Endophytic Pseudomonas Aeruginosa XGDE 7 from Mangrove Xylocarpus Granatum J. Koenig : Antibacterial, Antibiofilm, and Antioxidant Activities”. Journal of Research in Pharmacy 29/6 (November 1, 2025): 2633-2642. https://doi.org/10.12991/jrespharm.1798454.

JAMA

1.Hıdayah T, Hadı Wıbowo R, Septama AW, Sipriyadi S, Adfa M, Prastya ME, Sukmawinata E, Mustafa M, Farestiani E. Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities. J. Res. Pharm. 2025;29:2633–2642.

MLA

Hıdayah, Thoriqul, et al. “Secondary Metabolites of Endophytic Pseudomonas Aeruginosa XGDE 7 from Mangrove Xylocarpus Granatum J. Koenig : Antibacterial, Antibiofilm, and Antioxidant Activities”. Journal of Research in Pharmacy, vol. 29, no. 6, Nov. 2025, pp. 2633-42, doi:10.12991/jrespharm.1798454.

Vancouver

1.Thoriqul Hıdayah, Risky Hadı Wıbowo, Abdi Wira Septama, Sipriyadi Sipriyadi, Morina Adfa, Muhammad Eka Prastya, Eddy Sukmawinata, Muskhazli Mustafa, Eliza Farestiani. Secondary metabolites of endophytic Pseudomonas aeruginosa XGDE 7 from Mangrove Xylocarpus granatum J. Koenig : Antibacterial, antibiofilm, and antioxidant activities. J. Res. Pharm. 2025 Nov. 1;29(6):2633-42. doi:10.12991/jrespharm.1798454