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Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity

Year 2026, Volume: 30 Issue: 1, 357 - 366, 11.01.2026
https://doi.org/10.12991/jrespharm.1845209

Abstract

In the past several years, silver (Ag) nanoparticles (NPs) have made great progress because of their exceptional properties in a variety of applications. The green production method of nanoparticles has been gaining ground on conventional chemical and physical processes by eschewing the usage of costly devices and several harmful substances. Consequently, AgNPs have been produced in this study using Portulaca oleracea extract as reducing, capping, and stabilising agents. It is an easy, green, and environmentally friendly process. UV-visible spectroscopy, FTIR, XRD, and DLS were used to prove the stability and making of the synthesised Po-AgNPs. AgNPs production was demonstrated by the UV–vis spectra, which showed a transition from a colourless liquid to a brownish solution. FTIR (Fourier transform infrared spectroscopy) was used to show how the extract accessible functional groups contributed to the reduction and capping of NPs. The X-ray diffraction validated the synthesised NPs crystalline structure. The cumulant hydrodynamic diameter of Po-AgNPs was measured by DLS to be 368.6 nm, with a PdI of 0.433. The zeta potential value was found to be –34.3 mV, indicating that the Po-AgNPs are more stable and have negative charge. When biosynthesised Po-AgNPs were evaluated for antioxidant activity using DPPH tests, Po-AgNPs showed greater antioxidant activity. The results indicates that the stability, purity, degree of crystallinity, and cubic form of NPs produced by Portulaca oleracea extract are excellent. In general, more investigation is needed to examine the potential health benefits of the Ag-NP-based P. oleracea aqueous extract.

References

  • [1] Kiani BH, Arshad I, Najeeb S, Okla MK, Almanaa TN, Al-Qahtani WH, Abdel-Maksoud MA. Evaluation of biogenic silver nanoparticles synthesized from vegetable waste. Int J Nanomedicine. 2023;18:6527-6544. https://doi.org/10.2147/IJN.S432252.
  • [2] Erci F, Cakir-Koc R, Isildak I. Green synthesis of silver nanoparticles using Thymbra spicata L. var. spicata (zahter) aqueous leaf extract and evaluation of their morphology-dependent antibacterial and cytotoxic activity. Artif Cells Nanomed Biotechnol 2018; 46:150–158. https://doi.org/10.1080/21691401.2017.1415917.
  • [3] Barzinjy AA, Haji BS. Green synthesis and characterization of Ag nanoparticles using fresh and dry Portulaca oleracea leaf extracts: Enhancing light reflectivity properties of ITO glass. Micro Nano Lett 2024;19. https://doi.org/10.1049/mna2.12198.
  • [4] Abdel-Rahman MA, Alshallash KS, Eid AM, Hassan SE, Salih M, Hamza MF, Fouda A. Exploring the antimicrobial, antioxidant, and antiviral potential of eco-friendly synthesized silver nanoparticles using leaf aqueous extract of Portulaca oleracea L. Pharmaceuticals (Basel). 2024;17(3):317. https://doi.org/10.3390/ph17030317.
  • [5] Banerjee P, Satapathy M, Mukhopahayay A, Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: Synthesis, characterization, antimicrobial property, and toxicity analysis. Bioresour Bioprocess 2014;1. https://doi.org/10.1186/s40643-014-0003-y.
  • [6] Kumar A, Sreedharan S, Kashyap AK, Singh P, Ramchiary N. A review on bioactive phytochemicals and ethnopharmacological potential of purslane (Portulaca oleracea L.). Heliyon. 2021;8(1):e08669. https://doi.org/10.1016/j.heliyon.2021.e08669.
  • [7] De Souza PG, Rosenthal A, Ayres EMM, Teodoro AJ. Potential functional food products and molecular mechanisms of Portulaca oleracea L. on anticancer activity: A Review. Oxid Med Cell Longev. 2022;2022:7235412. https://doi.org/10.1155/2022/7235412.
  • [8] Voynikov Y, Gevrenova R, Balabanova V, Doytchinova I, Nedialkov P, Zheleva-Dimitrova D. LC-MS analysis of phenolic compounds and oleraceins in aerial parts of Portulaca oleracea L. J Appl Bot Food Qual. 2019;92:298–312. https://doi.org/10.5073/JABFQ.2019.092.041.
  • [9] Al-Otibi F, Alfuzan SA, Alharbi RI, Al-Askar AA, Al-Otaibi RM, Al Subaie HF, Moubayed NMS. Comparative study of antifungal activity of two preparations of green silver nanoparticles from Portulaca oleracea extract. Saudi J Biol Sci. 2022;29(4):2772-2781. https://doi.org/10.1016/j.sjbs.2021.12.056.
  • [10] Atrooz OM, Al-Maitah SZ. Characterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn. J Appl Biol Biotechnol 2022; 10:28–33. https://doi.org/10.7324/JABB.2022.100305.
  • [11] Salunke M, Wakure B, Wakte P. HR-LCMS assisted phytochemical screening and an assessment of anticancer activity of Sargassum squarrossum and Dictyota dichotoma using in vitro and molecular docking approaches. J Mol Struct. 2022;1270. https://doi.org/10.1016/j.molstruc.2022.133833.
  • [12] Salunke MA, Wakure BS, Wakte PS. High-resolution liquid chromatography and mass spectrometry (HR-LCMS) assisted phytochemical profiling and an assessment of anticancer activities of Gracilaria foliifera and Turbinaria conoides using in vitro and molecular docking analysis. J Biomol Struct Dyn. 2023;41(14):6476-6491. https://doi.org/10.1080/07391102.2022.2108495.
  • [13] Rajeshkumar S, Malarkodi C, Paulkumar K, Vanaja M, Gnanajobitha G, Annadurai G. Algae mediated green fabrication of silver nanoparticles and examination of ıts antifungal activity against clinical pathogens. Int J Metals. 2014; 2014: 692643. https://doi.org/10.1155/2014/692643.
  • [14] Suresh TC, Poonguzhali TV, Anuradha V, Ramesh B, Suresh G. Aqueous extract of Turbinaria conoides (j.agardh) kutzing mediated fabrication of silver nanoparticles used against bacteria associated with diabetic foot ulcer. Mater Today Proc. 2021; 43: 3038–3043. https://doi.org/10.1016/j.matpr.2021.01.376.
  • [15] Salunke MA, Wakure BS, Wakte PS. Phytochemical, UV-VIS, and FTIR Analysis of Gracilaria foliifera. Res J Pharm Technol. 2023; 16:1391–1394. https://doi.org/10.52711/0974-360X.2023.00229.
  • [16] Mohini S, Balaji W, Pravin W. Phytochemical analysis of Acanthophora najadiformis using high-resolution liquid chromatography mass spectrometry (HR-LCMS) and FTIR. J Pharm Negat Results. 2022; 13:2215–2218. https://doi.org/10.47750/pnr.2022.13.S06.287.
  • [17] Mohini SA, Balaji W, Pravin W. Phytochemical ınvestigation of brown algae Dictyota dichotoma collected from Ramanathapuram District, Tamilnadu. Int J Biol Pharm Allied Sci. 2021; 10:446–453. https://doi.org/10.31032/ijbpas/2021/10.12.1039.
  • [18] Salunke M, Wakure B, Wakte P. Phytochemical screening of marine brown algae Sargassum squarrossum Greville. Bull Env Pharmacol Life Sci 2022; 11:112–116.
  • [19] Jang MH, Lee S, Hwang YS. Characterization of silver nanoparticles under environmentally relevant conditions using asymmetrical flow field-flow fractionation (AF4). PLoS One. 2015;10(11):e0143149. https://doi.org/10.1371/journal.pone.0143149.
  • [20] Elamawi RM, Al-Harbi RE, Hendi AA. Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi. Egypt J Biol Pest Control 2018;28. https://doi.org/10.1186/s41938-018-0028-1.
  • [21] Raj CTD, Muthukumar K, Dahms HU, James RA, Kandaswamy S. Structural characterization, antioxidant and anti-uropathogenic potential of biogenic silver nanoparticles using brown seaweed Turbinaria ornata. Front Microbiol. 2023 ;14:1072043. https://doi.org/10.3389/fmicb.2023.1072043.
  • [22] Asif M, Yasmin R, Asif R, Ambreen A, Mustafa M, Umbreen S. Green synthesis of silver nanoparticles (AgNPs), structural characterization, and their antibacterial potential. Dose Response. 2022;20(1):15593258221088709. https://doi.org/10.1177/15593258221088709.
  • [23] Salunke M, Wakure B, Wakte P. A comparative analysis of antioxidant activity and total phenolic content of four seaweeds collected from southcoast of India. Int J Bot Stud 2022; 7:32–38.
  • [24] Salunke MA, Wakte PS, Wakure BS. An Assessment of anti-oxidant activity of marine algal polyphenols retrieved from Sargassum wightii, Turbinaria ornata, Gracilaria corticata and Gelidiella acerosa. Int J Pharm Sci Res 2021; 12:4964–4971. https://doi.org/10.13040/IJPSR.0975-8232.12(9).4964-71.
There are 24 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Research Article
Authors

Mohini Salunke 0000-0003-0759-2232

Smita Kumbhar 0000-0002-3030-9477

Wakure Balaji This is me 0000-0002-6027-9379

Submission Date August 21, 2024
Acceptance Date November 7, 2024
Publication Date January 11, 2026
Published in Issue Year 2026 Volume: 30 Issue: 1

Cite

APA Salunke, M., Kumbhar, S., & Balaji, W. (2026). Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity. Journal of Research in Pharmacy, 30(1), 357-366. https://doi.org/10.12991/jrespharm.1845209
AMA Salunke M, Kumbhar S, Balaji W. Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity. J. Res. Pharm. January 2026;30(1):357-366. doi:10.12991/jrespharm.1845209
Chicago Salunke, Mohini, Smita Kumbhar, and Wakure Balaji. “Green Biosynthesis and Characterization of Silver Nanoparticles Using Portulaca Oleracea and Evaluation of Antioxidant Activity”. Journal of Research in Pharmacy 30, no. 1 (January 2026): 357-66. https://doi.org/10.12991/jrespharm.1845209.
EndNote Salunke M, Kumbhar S, Balaji W (January 1, 2026) Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity. Journal of Research in Pharmacy 30 1 357–366.
IEEE M. Salunke, S. Kumbhar, and W. Balaji, “Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity”, J. Res. Pharm., vol. 30, no. 1, pp. 357–366, 2026, doi: 10.12991/jrespharm.1845209.
ISNAD Salunke, Mohini et al. “Green Biosynthesis and Characterization of Silver Nanoparticles Using Portulaca Oleracea and Evaluation of Antioxidant Activity”. Journal of Research in Pharmacy 30/1 (January2026), 357-366. https://doi.org/10.12991/jrespharm.1845209.
JAMA Salunke M, Kumbhar S, Balaji W. Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity. J. Res. Pharm. 2026;30:357–366.
MLA Salunke, Mohini et al. “Green Biosynthesis and Characterization of Silver Nanoparticles Using Portulaca Oleracea and Evaluation of Antioxidant Activity”. Journal of Research in Pharmacy, vol. 30, no. 1, 2026, pp. 357-66, doi:10.12991/jrespharm.1845209.
Vancouver Salunke M, Kumbhar S, Balaji W. Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity. J. Res. Pharm. 2026;30(1):357-66.