In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress

Mamoon H. Syed; Ayesha Yasmeen; Niraj Vyawahare; Smeeta Sudir; Tazneem Bachi; Sathish Kumar Mittapalli; Mohammed Jaffer Sadik; Ashok Kumar Uppuluru; Racha Spanadana; Roshan Safi

doi:10.12991/jrespharm.1909318

In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress

Abstract

A unique bioresource for folk and herbal remedies from antiquity to the present is Cissus quadrangularis. This study aimed to examine Cissus quadrangularis ethanolic stem extract’s potential as an in vivo anti-stress agent. Molecular docking and ADMET studies reveal good intermolecular interactions of many phytoconstituents with the protein to show drug-like properties. According to acute oral toxicity research, LD50 of ECQ is safe up to 700 mg/kg body per oral (p.o.) weight. Studies on unpredictable spontaneous stress (USS) show a corresponding decline in GSH, SOD, and CAT levels, as well as an increase in MDA and MPO levels compared to normal. The oxidative stress indices in USS models were significantly enhanced by treatment with extract dosages of 300 and 500 mg/kg. According to the results, MDA was considerably increased by USS (5.219 ± 0.156) compared to the control group’s MDA (2.984 ± 0.172). MDA levels were considerably reduced by both extract treatments (4.117 ± 0.165 and 3.889 ± 0.07, respectively; Gerifortetreated group: 3.171 ± 0.139). Histopathological study of extracts 300 and 500 mg/kg shows positive effects. These outcomes imply that extract was crucial in preventing oxidative damage following strenuous exercise, and the lower serum MPO levels might be the result of enhanced neutrophil intracellular redox conditions. As a result, our research strengthens the drug's current therapeutic potential for anti-stress or apoptogenic action and offers solid evidence in favor of its clinical application in contemporary medicine.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Mamoon H. Syed This is me
0000-0001-6627-7895
Saudi Arabia

Ayesha Yasmeen This is me
0000-0002-2954-5413
Saudi Arabia

Niraj Vyawahare This is me
0000-0001-6795-3314
India

Smeeta Sudir This is me
0000-0001-8682-303X
India

Tazneem Bachi
0000-0003-3220-2988
India

Sathish Kumar Mittapalli This is me
0000-0002-2028-2936
India

Mohammed Jaffer Sadik This is me
0000-0001-8175-1874
India

Ashok Kumar Uppuluru This is me
0000-0001-6777-3057
India

Racha Spanadana This is me
0009-0004-5059-3745
India

Roshan Safi ^* This is me
India

Publication Date

March 15, 2026

Submission Date

September 3, 2024

Acceptance Date

December 29, 2024

Published in Issue

Year 2026 Volume: 30 Number: 2

DOI

https://doi.org/10.12991/jrespharm.1909318

IZ

https://izlik.org/JA97ZU39CD

Cite

RIS / Bibtex

APA

H. Syed, M., Yasmeen, A., Vyawahare, N., Sudir, S., Bachi, T., Mittapalli, S. K., Sadik, M. J., Uppuluru, A. K., Spanadana, R., & Safi, R. (2026). In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress. Journal of Research in Pharmacy, 30(2), 535-552. https://doi.org/10.12991/jrespharm.1909318

AMA

1.H. Syed M, Yasmeen A, Vyawahare N, et al. In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress. J. Res. Pharm. 2026;30(2):535-552. doi:10.12991/jrespharm.1909318

Chicago

H. Syed, Mamoon, Ayesha Yasmeen, Niraj Vyawahare, et al. 2026. “In Silico and Animal Model Study of Cissus Quadrangularis Stem Ethanolic Extract for Unpredictable Spontaneous Stress”. Journal of Research in Pharmacy 30 (2): 535-52. https://doi.org/10.12991/jrespharm.1909318.

EndNote

H. Syed M, Yasmeen A, Vyawahare N, Sudir S, Bachi T, Mittapalli SK, Sadik MJ, Uppuluru AK, Spanadana R, Safi R (March 1, 2026) In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress. Journal of Research in Pharmacy 30 2 535–552.

IEEE

[1]M. H. Syed et al., “In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress”, J. Res. Pharm., vol. 30, no. 2, pp. 535–552, Mar. 2026, doi: 10.12991/jrespharm.1909318.

ISNAD

H. Syed, Mamoon - Yasmeen, Ayesha - Vyawahare, Niraj - Sudir, Smeeta - Bachi, Tazneem - Mittapalli, Sathish Kumar - Sadik, Mohammed Jaffer - Uppuluru, Ashok Kumar - Spanadana, Racha - Safi, Roshan. “In Silico and Animal Model Study of Cissus Quadrangularis Stem Ethanolic Extract for Unpredictable Spontaneous Stress”. Journal of Research in Pharmacy 30/2 (March 1, 2026): 535-552. https://doi.org/10.12991/jrespharm.1909318.

JAMA

1.H. Syed M, Yasmeen A, Vyawahare N, Sudir S, Bachi T, Mittapalli SK, Sadik MJ, Uppuluru AK, Spanadana R, Safi R. In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress. J. Res. Pharm. 2026;30:535–552.

MLA

H. Syed, Mamoon, et al. “In Silico and Animal Model Study of Cissus Quadrangularis Stem Ethanolic Extract for Unpredictable Spontaneous Stress”. Journal of Research in Pharmacy, vol. 30, no. 2, Mar. 2026, pp. 535-52, doi:10.12991/jrespharm.1909318.

Vancouver

1.Mamoon H. Syed, Ayesha Yasmeen, Niraj Vyawahare, Smeeta Sudir, Tazneem Bachi, Sathish Kumar Mittapalli, Mohammed Jaffer Sadik, Ashok Kumar Uppuluru, Racha Spanadana, Roshan Safi. In silico and animal model study of Cissus quadrangularis stem ethanolic extract for unpredictable spontaneous stress. J. Res. Pharm. 2026 Mar. 1;30(2):535-52. doi:10.12991/jrespharm.1909318