The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations

Fatih Demirci; Sümeyye Elif Kahya

The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations

Abstract

Recent years, particularly following the COVID-19 pandemic, a significant rise in fatigue, depression, and nervous system disorders was observed. While conventional pharmacotherapy is the standard for neuropsychiatric conditions, the use of herbal preparations is gaining prominence. Within the scope of this study, the potential anxiolytic effects of combined standardized herbal preparations were evaluated using the in vitro monoamine oxidase (MAO) inhibition assays. The essential oil of Lavandula angustifolia Mill. was analyzed and verified by GC/MS, where linalol (22.1%) and linalyl acetate (19.2%) was in compliance with European Pharmacopoeia. Whereas the Humulus lupulus L. flower aqueous ethanol extract contained 0.9% humulone according to the HPLC analyses. In vitro enzymatic assays showed that the H. lupulus extract inhibited MAO-A and MAO-B with IC50 values of 47.7 and 50 g/mL, respectively. Where the L. angustifolia essential oil showed IC50 69.4 and 55.1 g/mL inhibitions, respectively. Using the chechkerboard method, the combination of H. lupulus (1:4 ratio) and L. angustifolia (1:3 ratio) showed 95.5% inhibition of MAO-A, suggesting a syergistic effect, to the best of our knowledge for the first time. These initial experiements suggest that the combinations of lavender oil and hop strobile preparations may be utilized with the potential for development of standardized nervous system targetted phytotherapeutics.

Keywords

Supporting Institution

This study was supported by Anadolu University Scientific Research Projects Commission under the grant number BAP 2212S205.

Project Number

BAP 2212S205

Ethical Statement

This study does not require any ethical permission.

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Fatih Demirci ^*
0000-0003-1497-3017
Türkiye

Sümeyye Elif Kahya
0009-0002-5024-0021
Türkiye

Publication Date

August 3, 2026

Submission Date

April 6, 2026

Acceptance Date

July 4, 2026

Published in Issue

Year 2026 Volume: 30 Number: 4

IZ

https://izlik.org/JA95ZM93DD

Cite

RIS / Bibtex

APA

Demirci, F., & Kahya, S. E. (2026). The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations. Journal of Research in Pharmacy, 30(4), 1148-1156. https://izlik.org/JA95ZM93DD

AMA

1.Demirci F, Kahya SE. The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations. J. Res. Pharm. 2026;30(4):1148-1156. https://izlik.org/JA95ZM93DD

Chicago

Demirci, Fatih, and Sümeyye Elif Kahya. 2026. “The in Vitro Anxiolytic Potential of Standardized Lavandula Angustifolia Oil and Humulus Lupulus Extract Combinations”. Journal of Research in Pharmacy 30 (4): 1148-56. https://izlik.org/JA95ZM93DD.

EndNote

Demirci F, Kahya SE (August 1, 2026) The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations. Journal of Research in Pharmacy 30 4 1148–1156.

IEEE

[1]F. Demirci and S. E. Kahya, “The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations”, J. Res. Pharm., vol. 30, no. 4, pp. 1148–1156, Aug. 2026, [Online]. Available: https://izlik.org/JA95ZM93DD

ISNAD

Demirci, Fatih - Kahya, Sümeyye Elif. “The in Vitro Anxiolytic Potential of Standardized Lavandula Angustifolia Oil and Humulus Lupulus Extract Combinations”. Journal of Research in Pharmacy 30/4 (August 1, 2026): 1148-1156. https://izlik.org/JA95ZM93DD.

JAMA

1.Demirci F, Kahya SE. The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations. J. Res. Pharm. 2026;30:1148–1156.

MLA

Demirci, Fatih, and Sümeyye Elif Kahya. “The in Vitro Anxiolytic Potential of Standardized Lavandula Angustifolia Oil and Humulus Lupulus Extract Combinations”. Journal of Research in Pharmacy, vol. 30, no. 4, Aug. 2026, pp. 1148-56, https://izlik.org/JA95ZM93DD.

Vancouver

1.Fatih Demirci, Sümeyye Elif Kahya. The in vitro anxiolytic potential of standardized Lavandula angustifolia oil and Humulus lupulus extract combinations. J. Res. Pharm. [Internet]. 2026 Aug. 1;30(4):1148-56. Available from: https://izlik.org/JA95ZM93DD