Morpho-anatomical and palynological properties of Acanthoprasium integrifolium (Benth.) Ryding (Lamiaceae), endemic to Cyprus

Year 2023, Volume: 27 Issue: 1, 196 - 206, 28.06.2025

Aydan Fındık , Ayla Kaya

Abstract

This study aimed to describe the morphological, anatomical and palynological properties of Acanthoprasium integrifolium (Benth.) Ryding, which is endemic in Cyprus. Plant samples were collected from Northern Cyprus. Morphological observations were made using a binocular stereo microscope with a drawing tube. For anatomical studies, the samples were conserved in 70% alcohol. The transverse and surface sections were taken by hand with a help razor blade. The nutlet micromorphologies and pollen properties were examined using scanning electron microscopy. In morphological studies, the description of A. integrifolium was given in detail for the first time and the general appearance of plant and leaf, bract, bracteole, flower, calyx, corolla and nutlet shapes were supported by drawings. The stem and leaf characteristics of the plant were examined in anatomical studies. The stem is almost tetragonal-rounded in shape. The vascular bundles are well-developed in the corners. The pith area covers a large area and includes simple crystals. The leaf is bifacial type and hypostomatic. The pollen grains are tricolpate and prolate shaped. While the surface of pollen grains is reticulate, the nutlets have scalariform ornamentation.

Keywords

Acanthoprasium integrifolium , morphology , anatomy , nutlet , palynology

References

• 1. Meikle, R. D. Flora of Cyprus, Volume 1, The Bentham-Moxon Trust, Royal Botanic Gardens, Kew. 1977; 225.
• 2. Meikle, R. D. Flora of Cyprus, Volume 2, The Bentham-Moxon Trust, Royal Botanic Gardens, Kew. 1985.
• 3. Care Mediflora Web site. Project Mediterranean islands: Cyprus. http://www.caremediflora.eu/en/about/mediterranean_islands (accessed on 3 June 2022).
• 4. Hand, R., Hadjikyriakou, G.N., Christodoulou, C.S. Updated numbers of the vascular flora of Cyprus including the endemism rate. Cypricola. 2019; 13: 1-6.
• 5. Heywood, V.H. Flowering plants of the world. Oxford, Oxford University Press. 1978.
• 6. Bendiksby, M., Thorbek, L., Scheen, A.C., Lindqvist, C., Ryding, O. An updated phylogeny and classification of Lamiaceae subfamily Lamioideae. Taxon. 2011; 60(2): 471-484. [CrossRef]
• 7. Siadati, S., Saeidi Mehrvarz, S., Salmaki, Y. Nutlet micromorphology of the genus Marrubium L. and allies and its systematic implication (Lamiaceae: tribe Marrubieae). Nova Biologica Reperta. 2019; 6(3): 338-346. [CrossRef]
• 8. Hand, R. The effectiveness of seed-banked material in ex situ cultivation: an example from Cyprus. Flora Mediterranea. 2013; 23: 93-103. [CrossRef]
• 9. Abu-Asab, M. S., Cantino, P. D. Systematic implications of pollen morphology in subfamilies Lamioideae and Pogostemonoideae (Labiatae). Annals of the Missouri Botanical Garden. 1994; 81(4): 653–686. [CrossRef]
• 10. Metcalfe C.R, Chalk, L. Anatomy of the Dicotyledones. Vols Ⅰ & II. London: Oxford University Press. 1950.
• 11. Ezer, N., Şahin, F. P., Toker, M. C. Morphological and Anatomical Investigations of Ballota nigra L. subsp. anatolica PH Davis Used as Folk Medicine. Israel Journal of Plant Sciences. 1988; 47(1): 43-48. [CrossRef]
• 12. Şahin, F. P., Toker, M. C., Ezer, N. Botanical properties of a Mild Sedative: Ballota nigra L. subsp. nigra. FABAD Journal of Pharmaceutical Sciences. 2005; 30(2): 94-99.
• 13. Hatamneia, A.A., Khayami, M., Mahmudzadeh, A., Sarghein, S.H., Heidarih, M. Comparative Anatomical Studies of Some Genera of Lamiaceae Family in West Azarbaijan in Iran. Botany Research Journal. 2008; 1(3): 63-67.
• 14. Psaras, G. K., Rhizopoulou, S. Mesophyll structure during leaf development in Ballota acetabulosa. New phytologist. 1995; 131(3): 303-309. [CrossRef]
• 15. Yazgan, A. N., Yılmaz, G., Sever Yılmaz, B. Ballota acetabulosa (L.) Benth. bitkisi üzerinde anatomik çalışmalar. Ankara Eczacılık Fakültesi Dergisi. 2010; 39(4): 265-274. [CrossRef]
• 16. Uysal, İ. Ballota nigra L. subsp. anatolica Davis Endemik Taksonunun Morfolojisi, Anatomisi ve Ekolojisi Üzerine Araştırmalar. Eren Üniversitesi Fen Bilimleri Dergisi. 1997; 13(1-2): 67-77.
• 17. El-Deen Osman, A.K. Comparative Anatomical and Palynological Studies on Genus Ballota (Lamiaceae) from Egypt. Journal of Medicinal Plants Research. 2012; 6(47): 5797-5812.
• 18. Erdtman, G.Pollen morphology and plant taxonomy. IV. Labiatae, Verbenaceae and Avicenniaceae. Svensk Botanisk Tidskrift. 1945; 39: 279–285.
• 19. Abu-Asab, M. S., Cantino, P. D. Pollen morphology in the subfamily Lamioideae (Labiatae) and its phylogenetic implications. Advances in Labiatae Science. 1992; 97: 112.
• 20. Demissew, S., Harley, M.M. Trichome, seed surface and polen characters in Stachys (Lamioideae: Labiatae) in tropical Africa. Advances in Labiatae Science. 1992; 149-166.
• 21. Doaigey, A. R., El-Zaidy, M., Alfarhan, A., Milagy, A. E. S., Jacob, T. Pollen morphology of certain species of the family Lamiaceae in Saudi Arabia. Saudi Journal of Biological Sciences. 2018; 25(2): 354-360. [CrossRef]
• 22. Stearn, W.T. Botanical Latin. David & Charles Newton Abbot. London. 1978.
• 23. Google Earth. https://earth.google.com/web (accessed on 6 June 2022).