Sıçan İleumunda Kapsaisinin Neden Olduğu Morfometrik Değişikliklerin, IGF-I ve IGF-IR Ekspresyonlarının İncelenmesi

Abstract

Bu çalışma kapsaisin uygulaması sonrası ileumdaki histomorfolojik değişiklik ve endokrin, parakrin, otokrin etkiler yoluyla hücresel büyümeyi, farklılaşmayı ve apoptozu düzenlemede rol alan IGFI ve onun reseptörü olan IGFI-R’nün ifadesi araştırılmıştır. 21 günlük Sprague-Dawley sıçan Kontrol gurubu ve Kapsaisin alan tedavi grubu olarak 2’ye ayrıldı. Tedavi grubuna 20 gün boyunca deri altında 0,5mg/kg Kapsaisin uygulandı Deney sonunda ileumlar toplanarak dokular immünohistokimya (IGF-I-IGF-IR) ve morfometrik analizlerle değerlendirildi. Tedavi gruplarının sindirimi destekleyen parametrelerinden villus yüksekliği, kript derinliği, total mukozal kalınlığı, villus/kript oranı, yüzey emilim alanı istatistiki olarak arttı. Ayrıca kriptlerde ve düz kas tabakasında IGF-I ve IGFI-R’nün ifadesinde artış belirlendi. Ancak yüzey epitelinde kontrol grubuna göre herhangi bir istatistiki önem tespit edilmedi. Histomorfolojik parametrelerdeki artış ve hücrelerin mitotik olarak en yoğun olduğu kript bezlerinde görülen IGF-I ve reseptörünün ifadesinde artış Kapsaisinin IGF-I ve IGF-IR etki mekanizmasını etkilediği ve tedavinin sindirim sisteminde besin emilimini ve yararlanımını destekleyerek bağırsak morfolojisini olumlu yönde etkilediği sonucuna vardık.

Keywords

• İleum
• IGF-I
• IGF-IR
• Kapsaisin.

Investigation of Capsaicin-Induced Morphometric Changes, IGF-I and IGF-IR Expressions in Rat Ileum

Abstract

This study investigated the histomorphological changes in the ileum after capsaicin application and the expression of IGFI and its receptor IGFI-R, which play a role in regulating cellular growth, differentiation and apoptosis through endocrine, paracrine and autocrine effects. 21-day-old Sprague-Dawley rats were divided into 2 groups as the Control group and the Capsaicin-receiving treatment group. 0.5mg/kg Capsaicin was applied subcutaneously to the treatment group for 20 days. At the end of the experiment, the ileums were collected and the tissues were evaluated with immunohistochemistry (IGF-I, IGF-IR) and morphometric analyses. The parameters supporting digestion in the treatment groups, such as villus height, crypt depth, total mucosal thickness, villus/crypt ratio, and surface absorption area, increased statistically. In addition, an increase in the expression of IGF-I and IGF-IR was determined in the crypts and smooth muscle layer. However, no statistical significance was detected in the surface epithelium compared to the control group. The increase in histomorphological parameters and the increase in the expression of IGF-I and its receptor seen in the crypt glands where the cells are mitotically densest lead to the conclusion that capsaicin affects the mechanism of action of IGF-I and IGF-IR and that the treatment positively affects intestinal morphology by supporting nutrient absorption and utilization in the digestive system.

Keywords

• Ileum
• IGF-I
• IGF-IR
• Capsaicin

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