Evaluation of the Vitamin D Effect on Some Blood Parameters

Year 2025, Volume: 44 Issue: 2, 90 - 94, 31.12.2025

Ebru Özyurt , Duygu Udum , Songül Fidan Bulut , Murat Yalçın

https://doi.org/10.30782/jrvm.1797951

Abstract

Vitamin (Vit) D, which is produced by exposure of the skin to sunlight, is necessary for mineral metabolism and skeletal health, as well as having anti-inflammatory and immune boost effects in the organism. It is also known that melatonin, which regulates the sleep/wake cycle, circadian and seasonal rhythms, has immunostimulatory activity like Vit D. It is also known that there is a relationship between melatonin and Vit D at the level of the Vit D receptor, thus causing Vit D to increase its cellular activities. In the light of this information, it was aimed to investigate the effects of chronic Vit D mechanism on some blood parameters in rats in this study.
Spraque Dawley rats used in the study were divided into 4 groups. For 4 weeks, animals in the control, melatonin, Vit D, and Vit D restriction groups were chronically injected saline (1 ml/kg; ip), melatonin (10 mg/kg; ip), Vit D (2.5 μg/kg; ip), and saline (1 ml/kg; ip), respectively. Vit D restriction was applied to rats in the Vit D restriction group, both as a light source and through feeding. At the end of the experiment, 1 ml of EDTA blood sample was collected from the animals and hematocrit (PCV, %), hemoglobin amount (Hb, g/dl), red blood cell count (RBC, x106/mm3), white blood cell count (WBC, x103/mm3), leukocyte ratio (%), and erythrocyte sedimentation rate (ESR, mm/h) were measured.
The current findings demonstrate that chronic Vit D, melatonin use and chronic Vit D restriction causes the decrease in PCV and Hb without changing the other RBC related parameters. In terms of WBC, chronic Vit D, melatonin use and chronic Vit D restriction led to decrease in N/L ratio without altering number and other leucocytes ratios.

Keywords

Vitamin D , Vitamin D restriction , Melatonin , Erythrocytes , Leucocytes

Ethical Statement

2020-03/05

Project Number

TGA-2022-744

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