Evaluation of the Vitamin D Effect on Some Blood Parameters

Abstract

Vitamin (Vit) D, which is produced by exposure of the skin to sunlight, is necessary for mineral metabolism and skeletal health, as well as having anti-inflammatory and immune boost effects in the organism. It is also known that melatonin, which regulates the sleep/wake cycle, circadian and seasonal rhythms, has immunostimulatory activity like Vit D. It is also known that there is a relationship between melatonin and Vit D at the level of the Vit D receptor, thus causing Vit D to increase its cellular activities. In the light of this information, it was aimed to investigate the effects of chronic Vit D mechanism on some blood parameters in rats in this study. Spraque Dawley rats used in the study were divided into 4 groups. For 4 weeks, animals in the control, melatonin, Vit D, and Vit D restriction groups were chronically injected saline (1 ml/kg; ip), melatonin (10 mg/kg; ip), Vit D (2.5 μg/kg; ip), and saline (1 ml/kg; ip), respectively. Vit D restriction was applied to rats in the Vit D restriction group, both as a light source and through feeding. At the end of the experiment, 1 ml of EDTA blood sample was collected from the animals and hematocrit (PCV, %), hemoglobin amount (Hb, g/dl), red blood cell count (RBC, x106/mm3), white blood cell count (WBC, x103/mm3), leukocyte ratio (%), and erythrocyte sedimentation rate (ESR, mm/h) were measured. The current findings demonstrate that chronic Vit D, melatonin use and chronic Vit D restriction causes the decrease in PCV and Hb without changing the other RBC related parameters. In terms of WBC, chronic Vit D, melatonin use and chronic Vit D restriction led to decrease in N/L ratio without altering number and other leucocytes ratios.

Keywords

• Vitamin D
• Vitamin D restriction
• Melatonin
• Erythrocytes
• Leucocytes

Project Number

TGA-2022-744

Ethical Statement

2020-03/05

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