Effect of Central and Peripheral Injected Nesfatin-1 on Electrocardiography in Rats

Abstract

Nesfatin-1 is an anorexic nucleobindin-2 -derived peptide and it has directly and centrally effect on the heart. The current study was designed

to determine the effect of centrally and peripherally administered nesfatin-1 on electrocardiography (ECG) of healthy both fasted rats for 12

h and satiated rats fed ad libitum. In order to record ECG, the electrodes were placed limbs of at lead II under ketamine (50 mg/kg; im) and

xylazine (20 mg/kg; im) anesthesia mix.

Centrally administered different doses of nesfatin-1 (100 and 200 pmol; icv) resulted in dose- and time-dependently a statistically significant

increase (p <0.05) in T wave, Q-T interval, and R-R interval duration without changing in ECG waves’ amplitude in both satiated and fasted

rats. In a similar way, peripheral administration of nesfatin-1 (80 μg/kg; iv) in satiated rats prolonged statistically significant (p <0.05) T wave,

Q-T interval, and R-R interval without producing a change in ECG waves’ amplitude. Moreover, icv administered nesfatin-1 in fasted and

satiated rats, and iv injected nesfatin-1 in satiated rats induced a statistically significant decrease in heart rate (p <0.05).

In conclusion, our findings suggest that centrally and peripherally administrated nesfatin-1 caused a delay in T wave, Q-T interval and two

R-waves interval duration in ECG so that leading to a bradycardic effect in heart rate.

Keywords

• Nesfatin-1,Electrocardiography,Intracerebroventricular,Intravenous,Heart rate

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