Farklı Dozlardaki Gonadotropinlerin Ovaryum Follikül Sayıları Üzerine Etkisi

Year 2019, , 39 - 45, 18.07.2019

Guzin Pancaroglu Tuncay Ilhan , Hatice Erdost

https://doi.org/10.30782/uluvfd.416847

Abstract

The aim of our study is to determine the effect of PMSG and following hCG administration to seven-week-old mice follicle development

and corpus luteum formation by statistically methods. Seven-week-old, fourty BALB/c breed female mice were used in this study. Mice were

divided in to four groups randomly, and 0,2 ml buffer saline was injected to control group. PMSG hormone was administrated at doses of

2,5; 5 and 7,5 I.U. respectively to other three groups. hCG was injected 2,5; 5 and 7,5 I.U. subcutaneous doses respectively to the experiment

groups after 48 hours later than PMSG administration and 0,2 ml buffer saline was injected subcutaneously to control group. Ovaries were

collected and fixed for histological examination and Crossmon’s triple staining method was applied for examination of follicle and corpus

luteum developments in all experiment groups were more than control group. First experiment group had the most developing follicle number,

followed by third and second experiment group respectively and at least control group. When corpus luteums were evaluated in terms

of ovulation, it was seen that the third, second and first experiment groups were the most respectively and the control was the least. In all

procedures, follicular development and ovulation were increased depending on the applied dose of gonadotropin. As a result of this study; it

was decided, third experimental group had the most number of corpus luteum formation (7,5 I.U. PMSG and following 7,5 I.U. hCG subcutanous

administration) was the most appropriate superovulation procedure for BALB/c breed female mice.

Keywords

Süperovulasyon, PMSG, hCG, ovaryum, fare

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