A Phylogenetic and Genotyping Study of Bovine Herpesvirus Type 4 (BHV-4) in Turkey

Year 2020, , 61 - 66, 29.07.2020

Bahattin Taylan Koç

https://doi.org/10.30782/jrvm.641125

Abstract

Infertility
and reproductive infections are the huge problems for the diary management
throughout the world. Bovine herpesviruses act an enormous role in these
complicated problems. Bovine Herpesvirus Type 1 (BHV-1) is the most outstanding
herpesvirus causing genital and uterine tracts infections among other
reproductive viral agents, however, Bovine Herpesvirus Type 4 (BHV-4) is also responsible
in terms of similar symptoms and diseases. The main aims of the study are both
to investigate the underlying potential presence of BHV-4 in subclinical
uterine tract infection, and both to perform molecular and recombination
analyses. A herd including 25 repeat breeder cows were investigated by BHV-4.
Two out of them were determined BHV-4 infected after a series of Polymerase
Chain Reactions (PCRs) tests which able to amplify partial Glycoprotein B (gB)
and Thymidine Kinase (TK) gene regions. Obtained sequences were analyzed by
using phylogenetic and recombinational software, and two Maximum Likelihood
(ML) tree have been constructed. To results, novel Turkish BHV-4 sequences fell
into Genotype I in both constructed Maximum Likelihood (ML) phylogenetic trees,
however, no recombination evidence has been observed in relevant software. This
report is the first genotyping study of BHV-4 from Turkey. This study showed
that Turkish BHV-4 substantially originated from European strains and might be
observed in different clinical presentations. This suggests that BHV-4 should
be deeply investigated by further molecular techniques and included in
diagnostic panels for reproductive diseases viruses.

Keywords

Reproductive Infection, Bovine herpesvirus Type 4 (BHV-4), Repeat-breeder, Infertility, Genotyping, Turkey

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