Identification of Mesenchymal Stem Cells and Proliferation Properties

Year 2020, , 106 - 114, 30.12.2020

Ece Çerçi , Hatice Erdost

https://doi.org/10.30782/jrvm.659950

Abstract

Mesenchymal stem cells (MSCs) are undifferentiated cells that are highly abundant in animals and humans and have therapeutic efficacy. The aim of this study was to determine the MSC characteristics of passage 3 cells isolated from adipose tissue, immunolocalization of Ki-67 antibody, and to evaluate proliferation by cell growth analysis.
Passage 3 cells were differentiated into adipocytes, osteoblasts and chondroblasts and stained with Oil Red O, Alizarin Red and Alcian Blue techniques. MSCs characterization was identified by stem cell surface markers positive expression of CD 90 and CD 105 negative expression of CD 45 and CD 11b.
As a result; fat tissue origin passage in 3 cells have been shown that immunophenotypic characterization, differentiation in osteogenic, chondrogenic and adipogenic aspects, preserving optimal cellular hemostasis by Ki-67 positive immunpositive cells, PDT findings and therapeutically healthy mesenchymal stem cell proliferation.

Keywords

Stem Cell Differentiation, Immunophenotyping, Ki-67, Analyse of Cell Development

Mezenkimal Kök Hücrelerin Tanımlanması ve Proliferasyon Özellikleri

Abstract

Mezenkimal kök hücreler (MKH), hayvanlar ve insanlarda yüksek oranda bulunan ve terapötik etkinliğe sahip farklılaşmamış hücrelerdir. Bu çalışmada, yağ dokudan izole edilen pasaj 3 hücrelerinde, MKH özelliklerinin tanımlanması, Ki-67 antikorunun immunolokalizasyonu, hücre gelişim analizi ile proliferasyonun değerlendirilmesi amaçlandı.
Pasaj 3 hücreleri adiposit, osteoblast ve kondroblastlara farklılaştırılarak Oil Red O, Alizarin Red ve Alcian Blue teknikleri ile boyandı. MKH karakterizasyonu; kök hücre yüzey işaretleyicilerinden CD 90 ve CD 105 ile pozitif; CD 45 ve CD 11b ile negatif ekspresyonu tanımlandı.
Sonuç olarak çalışmada; yağ doku kökenli pasaj 3 hücrelerde; immünofenotipik karakterizasyonun, osteojenik, kondrojenik ve adipojenik yönde farklılaşmanın, Ki-67 immunpozitif hücrelerde, PDT bulgularında optimal hücresel hemostazisin korunduğu ve terapötik açıdan sağlıklı mezenkimal kök hücre proliferasyonunun gerçekleştiği gösterilmiştir.

Keywords

Kök Hücre Farklılaşması, İmmunofenotiplendirme, Ki-67, Hücre Gelişim Analizi

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