Immunohistochemical and Zymographic Analyses of Matrix metalloproteinase-2 (MMP-2) and Matrix Metalloproteinase-9 (MMP-9) in Bovine Papillomatous Digital Dermatitis

Year 2019, Volume: 38 Issue: 2, 83 - 87, 18.12.2019

Ahmet Akkoç

https://doi.org/10.30782/jrvm.616167

Abstract

Bovine
digital dermatitis (BDD) is an important health issue of dairy cattle. Painful papillomatous tissue
proliferations are observed in hind feet of affected animals. The pathogenesis
of this papillomatous lesion in BDD is still poorly understood. Matrix
metalloproteinases are a group of enzymes for tissue remodeling in both health
and disease. In the submitted study, immune expressions of MMP-2 and MMP-9
enzymes and their zymographic activities were evaluated. Higher immune staining
of MMP-2 and MMP-9 were found in BDD samples when compared with healthy skin
samples. The differences observed in MMP-2 and MMP-9 staining intensity and
distributions were statistically significant (p< 0.05, p<0.05 respectively).
Zymographic analyses revealed skin samples from both healthy and affected
animals had pro-active and active forms of MMP-2 and MMP-9 enzymes.
Immunohistochemistry results indicate that significant increase in MMP-2 and
MMP-9 enzymes in BDD samples. These results suggest MMP-2 and MMP-9 might have
roles in the pathogenesis of BDD.

Keywords

Bovine digital dermatitis, Matrix metalloproteinases, Immunohistochemistry, Zymography

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