Prognostic significance of PCNA, MMP-9 and p53 in bovine ocular squamous cell carcinomas

Yıl 2021, Cilt: 40 Sayı: 2, 98 - 105, 31.12.2021

Emin Karakurt , Uğur Aydın , Enver Beytut , Serpil Dağ , Celal Şahin Ermutlu , Özgür Aksoy , Hilmi Nuhoğlu , Ayfer Yıldız , Emre Kurtbaş

https://doi.org/10.30782/jrvm.937240

Cited By: 1

Öz

In this study, we aimed to evaluate PCNA, MMP-9 and p53 expressions according to differentiation degree of BOSCCs by immunohistochemical methods. The material of this study was composed of BOSCC biopsy samples taken from 30 cattle brought to our department. Tissue samples from cattles were fixed in 10% buffered formaldehyde solution, processed routinely, embedded in paraffin and sectioned at 5μm and stained with Hematoxylin & Eosine in order to detect histopathological changes. Sections were examined and photographed under a light microscope. Avidin-Biotin Peroxidase method was used as immunohistochemical method. We observed that the masses were nodular and cauliflower-like appearance. We found that the surfaces of the masses were highly hemorrhagic and ulcerative, sometimes covered with a purulent discharge. We defined cases with excessive and large numbers of keratin pearls, large tumoral islands, and evident squamous differentiation were defined as well-differentiated. In moderately-differentiated cases, we found that the number and size of keratin pearls decreased compared to well-differentiated cases. In addition, we observed that tumoral islets were smaller in these cases, similar to keratin pearls, and the number of poorly differentiated tumor cells increased. In poorly-differentiated cases, we determined that keratinization was either absent or formed in individual cells. As a result of statistical analysis, there was no statistically significant difference between good, moderate and poorly differentiated cases in terms of PCNA and MMP-9 expressions, but we found that the increase in p53 expression correlated with the degree of differentiation of the tumor. In conclusion, we think that p53 is a useful marker in determining the prognosis of BOSCCs.

Anahtar Kelimeler

cattle, ocular squamous cell carcinoma, mmp-9, p53, pcna

Kaynakça

• Referans1Prasanna Lakshmi M, Veena P, Suresh Kumar RV, et al. Clinical, pathological and immunohistochemical studies on bovine eye cancer. J Pharm Innov. 2020; 9(4):353-355.
• Referans2Sözmen M, Devrim AK, Sudağıdan M, et al. Significance of angiogenic growth factors in bovine ocular squamous cell carcinoma. J Comp Pathol. 2019; 170:60-69.
• Referans3Podarala V, Prasanna Lakshmi M, Venkata SKR, et al. Efficacy of BCG vaccine and Mitomycin C for the treatment of ocular squamous cell carcinoma in bovines. Res Vet Sci. 2020; 133:48-52.
• Referans4Kapoor J, Banga HS, Singh ND, et al. Studies on pathology of ocular tumors in bovine. Indian J Vet Pathol. 2020; 44(2):65-68.
• Referans5Pugliese M, Mazzullo G, Niutta PP, et al. Bovine ocular squamous cell carcinoma: a report of cases from the Caltagirone area, Italy. Vet Arh. 2014; 84(5):449-457.
• Referans6Vala H, Carvalho T, Pinto C, et al. Immunohistochemical studies of cytokeratins and differentiation markers in B-bovine ocular squamous cell carcinoma. Vet Sci. 2020; 7 (2):70.
• Referans7Jameel GH, Mohammed ZI, Taher MG, et al. TNF-alpha level, a marker for ivermectin induced immune modulation in cattle with ocular squamous cell carcinoma (BOSCC). Adv Anim Vet Sci. 2019; 7(6):441-446.
• Referans8Yakan S, Aksoy Ö, Karaman M, et al. Ocular squamous cell carcinoma case in three cattle. Harran Üniv Vet Fak Derg. 2017;6(2):180-185.
• Referans9Fornazari GA, Kravetz J, Kiupel M, et al. Ocular squamous cell carcinoma in Holstein cows from the South of Brazil. Vet World. 2017; 10(12):1413-1420.
• Referans10Taş A, Karasu A, Aslan L, et al. İki sığırda oküler yassı hücreli karsinom olgusu. YYU Veteriner Fakultesi Dergisi. 2009; 20(1):69-71.
• Referans11Tsujita H, Plummer CE. Bovine ocular squamous cell carcinoma. Vet Clin North Am Food Anim Pract. 2010; 26(3):511-529.
• Referans12Carvalho T, Vala H, Pinto C, et al. Immunohistochemical studies of epithelial cell proliferation and p53 mutation in bovine ocular squamous cell carcinoma. Vet Pathol. 2005; 42(1):66-73.
• Referans13Ceylan C, Ozyildiz Z, Yilmaz R, et al. Clinical and histopathological evaluation of bovine ocular and periocular neoplasms in 15 cases in Sanliurfa region. Kafkas Univ Vet Fak Derg. 2012; 18(3):469-474.
• Referans14Mestrinho LA, Pissarra H, Carvalho S, et al. Comparison of histological and proliferation features of canine oral squamous cell carcinoma based on intraoral location: 36 cases. J Vet Dent. 2017; 34(2):92-99.
• Referans15Martano M, Restucci B, Ceccarelli DM, et al. Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas. Oncol Lett. 2016; 11(1):399-404.
• Referans16Mestrinho LA, Faísca P, Peleteiro MC, et al. PCNA and grade in 13 canine oral squamous cell carcinomas: association with prognosis. Vet Comp Oncol. 2017; 15(1): 18-24.
• Referans17Mahale A, Alkatan H, Alwadani S, et al. Altered gene expression in conjunctival squamous cell carcinoma. Mod Pathol. 2016; 29(5):452-460.
• Referans18Di Girolamo N, Atik A, McCluskey PJ, et al. Matrix metalloproteinases and their inhibitors in squamous cell carcinoma of the conjunctiva. Ocul Surf. 2013; 11(3):193-205.
• Referans19Iovieno A, Lambiase A, Moretti C, et al. Therapeutic effect of topical 5-fluorouracil in conjunctival squamous carcinoma is associated with changes in matrix metalloproteinases and tissue inhibitor of metalloproteinases expression. Cornea. 2009; 28(7):821-824.
• Referans20Ng J, Coroneo MT, Wakefield D, et al. Ultraviolet radiation and the role of matrix metalloproteinases in the pathogenesis of ocular surface squamous neoplasia. Invest Ophthalmol Vis Sci. 2008; 49(12):5295-5306.
• Referans21Howell GM, Grandis JR. Molecular mediators of metastasis in head and neck squamous cell carcinoma. Head Neck. 2005; 27(8):710-717.
• Referans22Azarabad H, Gharagozlou MJ, Nowrouzian I, et al. p53 and Ki67 protein expression in ocular squamous cell carcinomas of dairy cattle. Iran J Vet Med. 2011; 5(4):226-231.
• Referans23Teifke JP, Löhr CV. Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs. J Comp Pathol. 1996; 114(2):205-210.
• Referans24Sironi G, Riccaboni P, Mertel L, et al. p53 protein expression in conjunctival squamous cell carcinomas of domestic animals. Vet Ophthalmol. 1999; 2(4):227-231.
• Referans25Albaric O, Bret L, Amardeihl M, et al. Immunohistochemical expression of p53 in animal tumors: a methodological study using four anti-human p53 antibodies. Histol Histopathol. 2001; 16(1):113-121.
• Referans26Gharagozlou MJ, Hekmati P, Ashrafihelan J. A clinical and histopathological study of ocular neoplasms in dairy cattle. Vet Arh. 2007; 77(5):409-426.
• Referans27Al-Asadi RN. A survey and treatment of ocular carcinomas in Iraqi dairy cows from (1987-2012). Kufa j vet Sci. 2012; 3(2):66-77.
• Referans28Rama Devi V, Veeraiah G, Annapurna P, et al. Squamous cell carcinoma of ear in an Indian water buffalo (Bubalus bubalis). Braz J Vet Pathol. 2010; 3(1):60-62.
• Referans29Chen YC, Chang SC, Huang YH, et al. Expression and the molecular forms of neutrophil gelatinase-associated lipocalin and matrix metalloproteinase 9 in canine mammary tumours. Vet Comp Oncol. 2019; 17(3):427-438.
• Referans30Zhang G, Luo X, Sumithran E, et al. Squamous cell carcinoma growth in mice and in culture is regulated by c-Jun and its control of matrix metalloproteinase-2 and -9 expression. Oncogene. 2006; 25(55):7260-7266.