Effects of Methionine and Cysteamine on Spermatological Parameters of Bee Spermatozoa Post Freezing-Thawing

Yıl 2021, Cilt: 40 Sayı: 2, 120 - 124, 31.12.2021

Selim Alçay Selvinar Seven-çakmak Ahmet Aktar İbrahim Çakmak

https://doi.org/10.30782/jrvm.991436

Öz

In this study, it was aimed to determine the effects of methionine and cysteamine in the cryopreservation of drone semen (Apis mellifera). Semen samples were collected from sexually mature drones and pooled. The pooled semen was then divided into five equal volumes and diluted with diluents added at different concentrations of methionine (2.5/5 mMol), cysteamine (2.5/5 mMol) and control. Motility values post thaw; Met5, Cys2,5 and Cys5 groups had a possitive effect on compared to the the control group (P<0.05). It was observed that the antioxidant groups protected the plasma membrane integrity better than the control group (P<0.05). Acrosomal integrity rates was better in the methionine groups compared to the control group (P<0.05). Membrane lipid peroxidation was analyzed by measuring malondialdehyde (MDA) concentration after dissolution. It was observed that antioxidant-containing extenders had beneficial effects on honey bee semen parameters. Considering all semen parameters; The Met5 group was found to be the most effective group for freezing drone semen.

Anahtar Kelimeler

Honey Bee, Semen, Cryopreservation, Antioxidant

Arı Spermasının Dondurma-Çözdürme Sonrası Spermatolojik Parametreleri Üzerine Metiyonin ve Sisteaminin Etkileri

Öz

Bu çalışmada arı spermasının (Apis mellifera) dondurulmasında metiyonin ve sisteaminin etkilerinin belirlenmesi amaçlanmıştır. Sperma cinsel olarak olgun erkek arılardan toplandı ve pooling yapıldı. Pooling yapılan sperma beş eşit hacme bölündü ve farklı konsantrasyonlarda metiyonin (2.5 / 5 mMol) ve sisteamin (2.5 / 5 mMol) içeren ve içermeyen (kontrol) sulandırıcılar ile sulandırıldı. Eritme sonrası motilite değerleri; Met5, Sist2,5 ve Sist5 gruplarında kontrol grubuna göre daha üstün bulundu (P<0.05). Plazma membran bütünlüğünü antioksidan grupları kontrol grubuna göre daha iyi koruduğu görüldü (P<0.05). Akrozomal bütünlük oranları metiyonin gruplarında kontrol grubuna göre daha üstün bulundu (P<0.05). Membran lipid peroksidasyonu eritme sonrası malondialdehit (MDA) konsantrasyonu ölçülerek analiz edildi. Antioksidan içeren sulandırıcıların bal arısı sperm parametreleri üzerinde faydalı etkilere sahip olduğu görüldü. Tüm sperm parametreleri göz önüne alındığında; Met5 grubu arı spermasının dondurulmasında en etkili grup olduğu görüldü.

Anahtar Kelimeler

Bal Arısı, Sperma, Dondurma, Antioksidan

Kaynakça

• 1. Seven İ, Yeninar H. Elaziğ yöresindeki aricilik işletmelerinin hastalik, parazit ve zararlilar yönünden incelenmesi. e-Journal of New World Sciences Academy. 2010; 5(2): 52-56.
• 2. Cobey S. Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance. Apidologie. 2007; 38: 390-410.
• 3. Woyke J, Jasınskı. Influence of age of drones on the results of instrumental insemination of honey bee queen. Apidologie 1978; 9(3): 203–211.
• 4. Genç F. Balarılarında koloni performansını etkileyen faktörler. Teknik Arıcılık. 1990; 27: 18–26.
• 5. Güler A. Bal Arısı (Apis mellifera L.) Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Ders Kitabı (55). Samsun. 2006.
• 6. Stankus T. A review and bibliography of the literature of honey bee colony collapse disorder: A poorly understood epidemic that clearly threatens the successful pollination of billions of dollars of crops in America. J Agric Food Inform. 2008; 9: 115-143.
• 7. Ortman K, Rodriguez-Martinez H. Membrane Damage during Dilution, Cooling and Freezing-Thawing of Boar Spermatozoa Packaged in Plastic Bags. Journal of Veterinary Medicine Series A. 1994; 41: 37-47.
• 8. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of Antioxidant Defenses Are Decreased in Bovine Spermatozoa after a Cycle of Freezing and Thawing. Mol Reprod Dev. 2000; 55: 282-8.
• 9. Nur Z, Zık B, Ustuner B, Sağırkaya H, Ozguden CG. Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology. 2010; 73: 1267-1275.
• 10. Watson PF. The roles of lipid and protein in the protection of ram spermatozoa at 5 degrees C by egg-yolk lipoprotein. J Reprod Fertil. 1981; 62(2): 483-92.
• 11. Donnelly ET, Mcclure N, Lewis SEM. Glutathione and hypotaurine in vitro: motility, DNA integrity and production of reactive oxygen species. Mutagenesis. 2000; 15: 61-68.
• 12. Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl. 2005; 26: 654-60.
• 13. Burley LM, Fell RD, Saacke RG. Survival of honey bee (Hymenoptera: Apidae) spermatozoa incubated at room temperature from drones exposed to miticides. J Econ Entomol. 2008; 101(4): 1081-1087.
• 14. Collins AM, Donoghue AM. Viability assessment of honey bee, Apis mellifera, sperm using dual fluorescent staining. Theriogenology. 1999; 51(8): 1513-1523.
• 15. Alcay S, Ustuner B, Çakmak İ, Çakmal S, Nur Z. Effects of various cryoprotective agents on post thaw drone semen quality. Kafkas Univ Vet Fak Derg. 2015; 21(1): 31-35.
• 16. Alcay S, Toker MB, Onder NT, Gokce E. Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa. Cryobiology. 2017; 74: 81-85.
• 17. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990; 186: 407-421.
• 18. Alcay S, Cakmak S, Cakmak I, Mulkpinar E, Gokce E, Ustuner B, Sen H, Nur Z. Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders. Cryobiology. 2019; 87: 28-31.
• 19. Alcay S, Toker MB, Gokce E, Ustuner B, Onder NT, Sagırkaya H. Successful ram semen cryopreservation with lyophilized egg yolk-based extenders. Cryobiology, 2015; 71: 329–333.
• 20. Hopkins BK, Herr C. Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie. 2010; 41: 548-556.
• 21. Yániz JL, Palacín I, Vicente-Fiel S, Gosálvez J, López-Fernández C, Santolaria P. Comparison of membrane-permeant fluorescent probes for sperm viability assessment in the ram. Reprod Domest Anim. 2013; 48: 598–603.
• 22. Gokce E, Alcay S, Gul Z. Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5ºC. Ankara Univ Vet Fak Derg. 2017; 64: 313-320.
• 23. I. El-Kon. Testing usability of bovine serum albumin (BSA) for preservation of Egyptian Buffalo Semen. Environ Sci. 2011; 11: 495–502.
• 24. Taylor MA, Guzmán Novoa E, Morfin N, Buhr MM. Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology. 2009; 72: 149–159.
• 25. Ustuner B, Alcay S, Toker MB, Nur Z, Gokce E, Sonat FA, Gul Z, Duman M, Ceniz C, Uslu A, Sagirkaya H, Soylu MK. Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender. Animal Repro Sci. 2016; 164: 97-104.
• 26. Alcay S, Cakmak S, Cakmak I, Mulkpinar E, Toker MB, Ustuner B, Sen H, Nur Z. Drone semen cryopreservation with protein supplemented TL-hepes based extenders. Kafkas Univ Vet Fak Derg. 2019; 25(4): 553-557.