Detection of the effect of the KISS1 gene on reproductive parameters in Saanen goats

Yıl 2024, Cilt: 43 Sayı: 1, 69 - 75, 02.09.2024

Deniz Dinçel , Mehmed Berk Toker , İbrahim Doğan

https://doi.org/10.30782/jrvm.1449054

Öz

The KISS1 gene, which activates G protein-coupled receptor-54 (GPR54) and plays a crucial role in the neuroendocrine regulation of GnRH secretion, is known to encode a family of neuropeptides called kisspeptins. Therefore, the KISS1 gene is thought to be associated with sexual maturity, offspring development, and estrus. The g.2124T>A polymorphism of the KISS1 gene, is believed to affect the reproductive system, has been studied in Indonesian goat breeds, Damascus, Zarabi, Baladi, Gaddi, Kaligesing, Guanzong, Xinong Saanen and Boer goat breeds until now. These studies are generally related determining polymorphism frequencies or the effect of genotype on litter size. The study aims to determine the allele and genotype frequencies of the KISS1 gene g.2124T>A SNP by the PCR-RFLP method and define the relationship between the genotypic structures and reproductive parameters such as luteal growth, maximum corpus luteum diameter, and mating rate in Saanen goats (n=30). According to the results, the genotype frequencies in Saanen goats for the KISS1 gene g.2124T>A were 10.35%, 55.17%, and 34.48% for AA, TT and AT, respectively. Moreover, the genotypic structure did not have a statistically significant effect on the investigated fertility characteristics. The average values of expected heterozygosity (He), observed heterozygosity (Ho), the effective number of alleles (Ne), fixation index values (FIS), and the polymorphism information content (PIC) were 0.3996, 0.3448, 1.6655, 0.8629 and 0.855, respectively. The Hardy-Weinberg chi square (χ2) value was found to be 0.5435. In conclusion, it was found necessary to study the KISS1 - g.2124T>A polymorphism in larger herds with different gene pools as a reason for the existence of genotypic variation and the narrow population size.

Anahtar Kelimeler

Fertility, Goat, KISS1, Saanen, Reproduction

Kaynakça

• 1. Fatet A, Pellicer-Rubio MT, Leboeuf B. Reproductive cycle of goats. Anim Reprod Sci, 2011; 124: 211–219.
• 2. Mohammed Muayad TA, Haniza MZH, Husni I. Reproductive performance of different goat breeds in Malaysia. Indian J Anim Res. 2019; 53(1): 24-27.
• 3. Tenorio Filho F, Santos MHB, Carrazzoni PG, Paula- Lopes FF, Neves JP, Bartolomeu CC, Lima PF, Oliveira MAL. Follicular dynamics in Anglo-Nubian goats using transrectal and transvaginal ultrasound. Small Rum Res. 2007; 72: 51–56.
• 4. Dogan I, Toker MB, Alcay S, Udum Kucuksen D. Effect of GnRH treatment following a short-term estrus induction protocol on estrus and ovulation in Saanen goats, during the transitional period. J Hellenic Vet Med Soc. 2020; 71(4): 2569-2576.
• 5. Kim KW, Lee J, Kim KJ, Lee ED, Kim SW, Lee SS, Lee SH. Estrus synchronization and artificial insemination in Korean black goat (Capra hircus coreanae) using frozen-thawed semen. J Anim Sci Technol. 2021; 61(1): 36-45.
• 6. Wen X, Liu L, Li S, Lin P, Chen H, Zhou D, Tang K, Wang A, Jin Y. Prostaglandin F2a Induces Goat Corpus Luteum Regression via Endoplasmic Reticulum Stress and Autophagy. Front in Physiol 2022; 11: 868.
• 7. Alkan KK, Alkan H, Kaymaz. The effect of anti-müllerian hormone and progesterone concentrations on superovulation response and embryo yield in goats. Theriogenology 2020; 143: 1-9.
• 8. Jiemtaweeboon S, Shirasuna K, Nitta A, Kobayashi A, Schubert HJ, Shimizu T, Miyamoto A. Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow. Reprod Biol Endocrinol. 2011; 9: 79.
• 9. Rahawy MA, AL-Mutar HA. Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats. Vet World. 2021; 14(8): 1995-2001.
• 10. An XP, Han P, Hou JX, Zhao HB, Yan Y, Ma T, Fang F, Meng FX, Song Y X, Wang JG, Cao BY. Molecular cloning and characterization of KISS1 promoter and effect of KISS1 gene mutations on litter size in the goat. Genetics and molecular research GMR 2013b; 12(4): 4308–4316.
• 11. Othman OE, Darwish HR, Abou-Eisha A, El-Din AE, Abdel-Samad MF. DNA characterization and polymorphism of KISS1 gene in Egyptian small ruminant breeds. Afr J Biotechnol. 2015; 14(30): 2335-2340.
• 12. Gaytan F, Gaytan M, Castellano JM, Romero M, Roa J, Aparicio B, Garrido N, Sanchez-Criado JE, Millar RP, Pellicer A, Fraser HM, Tena-Sempere M. KiSS-1 in the mammalian ovary: distribution of kisspeptin in human and marmoset and alterations in KiSS-1 mRNA levels in a rat model of ovulatory dysfunction. Am J of Physiol Endocrinol Metab 2009; 296: 520-531.
• 13. An X, Ma T, Hou J, Fang F, Han P, Yan Y, Zhao H, Song Y, Wang J, Cao B. Association analysis between variants in KISS1 gene and litter size in goats. BMC Genetics 2013a; 14: 63.
• 14. Dhillo WS. Kisspeptin: a novel regulator of reproductive function. J Neuroendocrinol. 2008; 20: 963–970.
• 15. Seminara SB, Messager S, Chatzidaki EE, Thresher RR, Acierno, JS Jr, Shagoury JK, Bo-Abbas Y, Kuohung W, Schwinof, KM, Hendrick AG, Zahn D, Dixon J, Kaiser UB, Slaugenhaupt SA, Gusella JF, O'Rahilly S, Carlton MB, Crowley WF Jr, Aparicio SA, Colledge WH. The GPR54 gene is a regulator of puberty. New Eng J Med. 2003; 349: 1614–1627.
• 16. Roa J, Tena-Sempere M. KiSS-1 system and reproduction: comparative aspects and roles in the control of female gonadotropic axis in mammals. Gen Comp Endocrinol. 2007; 153 (1-3): 132-140.
• 17. Kafa İM, Eyigör Ö. Kisspeptinler ve Kisspeptin Nöronları: Üreme Sistemi Üzerine Etkileri ve Hipotalamik Yerleşimleri. Uludağ Üniversitesi Tıp Fakültesi Dergisi 2011; 37(1): 53-60.
• 18. Maitra A, Sharma R, Ahlawat S, Tantia MS, Roy M, Prakash V. Association analysis of polymorphisms in caprine KiSS1 gene with reproductive traits. Anim Reprod Sci. 2014; 151(1-2): 71–77.
• 19. Mekuriaw G, Mwacharo JM, Dessie T, Mwai O, Djikeng A, Osama S, Gebreyesus G, Kidane A, Abegaz S, Tesfaye K. Polymorphism analysis of kisspeptin (KISS1) gene and its association with litter size in Ethiopian indigenous goat populations. Afr J Biotechnol. 2017; 16(22): 1254-1264.
• 20. Sahoo SS, Mishra C, Kaushik R, Rout PK, Singh MK, Bhusan S, Dige MS. Association of aSNP in KISS 1 gene with reproductive traits in goats. Biol Rhythm Res. 2021; 52(6): 922–933
• 21. Febriana A, Sutopo S, Kurnianto E, Widiyanto A. A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds. Trop Anim Sci J. 2022; 45(3): 255-269.
• 22. El-Tarabany MS, Zaglool AW, El-Tarabany AA, Awad A. Association analysis of polymorphism in KiSS1 gene with reproductive traits in goats. Ani Reprod Sci. 2017; 180: 92–99.
• 23. Sankhyan V, Thakur YP, Kumar Dogra P. Association of kisspeptin gene (KISS1) with litter size in migratory Gaddi goats in western Himalayan state of Himachal Pradesh. Indian J of Anim Sci 2019; 89(12): 1352-1355.
• 24. Hardyta G, Widayati DT, Maharani D. Association of SNP T125A on KiSS1 gene with reproduction hormone levels in Kaligesing goat. J Indones Trop Anim Agri 2020; 45(4): 253-260.
• 25. Kaymakçı M. Keçi Irkları. In: Kaymakçı M, ed. İleri Keçi Yetiştiriciliği. 1sted. İzmir Meta Basım Matbaacılık, 2013: 17-40.
• 26. Green M, Sambrook J. Analysis of DNA. In: Molecular Cloning: A Laboratory Manual. 4th ed. Cold Spring Harbor Laboratory Press, New York, 2012: 350-370.
• 27. Nei M and Roychoudhury AK. Sampling variances of heterozygosity and genetic distance. Genetics 1974; 76(2): 379-390.
• 28. Botstein, D, White, RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Amer. J. Hum. Genet. 1980; 32: 314–331.
• 29. Yeh F, Yang RC, Boyle T. PopGene (v.1.32), Microsoft Windows-based freeware for population genetic analysis. 2000.
• 30. Wiltbank MC, Mezera MA, Toledo MZ, Drum JN, Baez GM, Garcia-Guerra A, Sartori R. Physiological mechanisms involved in maintaining the corpus luteum during the first two months of pregnancy. Anim Reprod. 2018; 15: 805-821.
• 31. Arikan Ş and Yigit AA. Changes in the size distribution of goat steroidogenic luteal cells during pregnancy. Small Rum Res. 2003; 47: 227-231.
• 32. Sangha GK, Sharma RK, Guraya SS. Biology of corpus luteum in small ruminants. Small Rum Res. 2002; 43: 53-64.
• 33. Jiang YF, Hsu MC, Cheng CH, Tsui KH, Chiu CH. Ultrastructural changes of goat corpus luteum during the estrous cycle. Anim Reprod Sci. 2016; 170: 38-50.
• 34. Mishra GK, Patra MK, Sigh LK, Upmanyu V, Chakravarti S, Karkalan M, Singh SK, Das GK, Kumar H, Krisnaswami N. Kiss1 and its receptor: molecular characterization and immunolocalization in the hypothalamus and corpus luteum of the buffalo. Anim Biotechnol. 2019; 30(4): 342-351.
• 35. Smith JT, Li Q, Pereira A, Clarke J. Kisspeptin Neurons in the Ovine Arcuate Nucleus and Preoptic Area Are Involved in the Preovulatory Luteinizing Hormone Surge. Endocrinology. 2019; 150(12): 5530-5538.
• 36. Estrada KM, Clay CM, Pompolo S, Smith JT, Clarke IJ. Elevated KiSS-1 Expression in the Arcuate Nucleus Prior to the Cyclic Preovulatory Gonadotrophin-Releasing Hormone⁄Lutenising Hormone Surge in the Ewe Suggests a Stimulatory Role for Kisspeptin in Oestrogen- Positive Feedback. J Neuroendocrinol. 2006; 18: 806-8