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RESEARCH ON THE COMPOSITION AND CYTOTOXIC ACTIVITY OF PINUS BRUTIA GUM

Yıl 2018, Cilt 2, Sayı 1, 39 - 48, 31.01.2018

Öz

Pinus Brutia Ten., traditionaly used as sugar regulator, antioxidant and antitumoral is consumed by chewing in Turkey. The aim of the study is to investigate the components and the effect of the gum. The base of the study depends on the way of comsumption of the resin. For in-vitro analysis, artificial salvia is used. The samples is stayed in artificial salvia at 37° C in ultrasonic bath for  different time period and then determined by U-HPLC, PDA detector. Three samples were prepared from each sample and three injections were made. Caffeic acid  was investigate in the samples. The optimum waiting period is set at 5 hours, which is considered to be a possible period since the  gum adheres to the tooth and is a hardly soluble substance. Considering the anticarcinogenic effect of caffeic acid, cytotoxic test was carried out on specimens which were kept in saliva for 24 hours acid. After incubation of the saliva extract that including 10, 20, 50 µM caffeic acid  in liver cancerous cells 1, 3, 6 hours, cell viability was observed.

Kaynakça

  • REFERENCES ARIKTULUK, ZC, EZER, N. 2012, Halk arasında diyabete karşı kullanılan bitkiler. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 32(2): 179-208. ATICI, E.,2007, Tıp tarihinde kanser ve lösemi. Türk Onkoloji Dergisi, 22(4): 197-204. AVNI, H., BEHXHET, M., DASHNOR, N., HYRMETE, S., et.al., 2016, Essensial oil composition og pinus peuce Griseb. needles and twings from two national parks of Kosova. The Scientific World Journal, PMC49927553. BRUGGISSER, R., VON DAENIKEN, K., JUNDT, G., SCHAFFNER, W., 2002, Interference of plant extract phytoestrogens and antioxidants with MMT tetrazolium assay. Planta Med., 68(5), 445-448. CAN, G., KALAYCI, Ş, KAPLAN, R., 2006, Üç farklı kompozitin yapay tükürük ortamında flor salınım değerlerinin incelenmesi. Atatürk Üniv. Diş Hek. Fak. Derg., 33(1):33-38. DURMUS, M., YILMAZ, H.R., UZ, E., OZCELİK, N., 2008, The Effect of Caffeic Acid Phenethyl Ester (CAPE) Treatment on Levels of MDA, NO and Antioxidant Enzyme Activities in Retinas of Streptozotocin-Induced Diabetic Rats, Turk J Med Sci., 38 (6): 525-530 ICONOMOU, N., VALKANAS, G., BÜCHI, J., 1964, Composition of gum turpentines of Pinus halepensis and Pinus brutia grown in Greece [J]. J Chromatogr, 16: 29-33. İLHAMİ, G., 2006, Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology, 217(2-3):213–220. INCE, I., YESİL-CELİKTAS, O., KARABAY-YAVASOGLU, NU., ELGIN, G., 2009, Effects of Pinus brutia bark extract and Pycnogenols in arat model of carrageenan induced inflammation, Phytomedicine, 16 1101–1104. KIM, H.G., HAN, E.H., IM, J.H., LEE, E.J., JIN, S.W., 2015, Caffeic acid phenethyester inhibits 3-MC induced CYP1A1 expression through induction of hypoxio-inducible factor-1α. Biochem. Biophys. Research Comm., 465: 562-568. KIM, Y.M., JEONG, Y.K., WANG, M.H., LEE, W.Y., RHEE H.I., 2005, Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition. 21(6):756-61. KIM, Y.M., WANG, M.H., RHEE, H.I., 2004, A novel alpha-glucosidase inhibitor from pine bark. Carbohydr Res., 339(3):715-7. MAIMOONAA, A., NAEEMA, I., SADDİQEA, Z., JAMEELB, K., 2011, A review on biological, nutraceutical and clinical aspects of French maritime pine bark extract. J. Ethnopharm., 133: 261-277. MOSMANN,T., 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods., 65(1-2):55-63. OKTAR, S., AYDIN, M., YONDEN, M., ALCIN, E., İLHAN,S. etal., 2010, Effects of caffeic acid phenethyl ester on isoproterenol-induced myocardial infarction in rats. Anadolu Kardiyol Der., 10: 298-302. OLTHOF, M.R., HOLLMAN, P.C., KATAN, M.B., 2001, Chlorogenic acid and caffeic acid are absorbed in humans. J. Nutr., 131 (1): 66–71. OROIAN, M., ESCRICHE, I., 2015, Antioxidants: Characterization, natural sources, extraction and analysis. Food Research International, 74 10–36. RAJENDA PRASAD, N., KARTHIKEYAN, A., KARTHIKEYAN, S., REDDY, B.V., 2011, Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell line". Mol Cell Biochem, 349 (1-2): 11–9. RISS, T.L., MORAVEC,R.A., NILES A.L. et.al., 2013, Cell viability assays: Assay Guidance manuel., http://www.ncbi.nlm.nih.gov/books/NBK 144065/?report=reader) SAÇLI, S., AKALIN, E., 2001, Preliminary ethnobotanical study from Kaz dağı (Balıkesir/Çanakkale) I: Uses and vernacular names, İstanbul Eczacılık Fakültesi Mecmuası, 34 (2): 9. SATIL, F., SELVI, S., POLAT, R. 2011, Ethnic uses of pine resin production from Pinus brutia by native people on the Kazdağ Mountain (Mt. Ida) in Western Turkey [J]. J Food Agric Envir, 9 (3-4): 1059-1063. SCHILLER, G., GRUNWALD, C.1987, Cortex resin monoterpene composition in Pinus brutia provenances grown in Israel [J]. Biochem Syst Ecol., 15 (4): 389-394. ULUKANLI, Z.,KARABÖRKLÜ, S, BOZOK, F., ATES, B., ERDOGAN, S., et. al., 2014, Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus Brutia and Pinus pinea resin. Chinese Journal of Natural Medicines, 12(12): 901-910. YANG, N.Y, LIU, L., TAO, W.W., et al., 2010, Diterpenoids from Pinus massoniana resin and their cytotoxicity against A431 and A549 cells [J]. Phytochemistry, 71 (13): 1528-1533. YESIL-CELIKTAS, O., ISLETEN, M., VARDAR-SUKAN, F, CETIN, O., 2010, In vitro release kinetics of pinebark extract enriched orange juice and the shelf stability, British Food Journal, 112 ( 10):1063 - 1076 . YONEI, Y., MIZUNO, Y., KATAGIRI, E., 2004. Effects of cosmetics therapy using isoflavone and pine bark extract on the skin and QOL: a double-blind placebo-controlled trial. Anti-Aging Med. Res. 1, 48–58.

Yıl 2018, Cilt 2, Sayı 1, 39 - 48, 31.01.2018

Öz

Kaynakça

  • REFERENCES ARIKTULUK, ZC, EZER, N. 2012, Halk arasında diyabete karşı kullanılan bitkiler. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 32(2): 179-208. ATICI, E.,2007, Tıp tarihinde kanser ve lösemi. Türk Onkoloji Dergisi, 22(4): 197-204. AVNI, H., BEHXHET, M., DASHNOR, N., HYRMETE, S., et.al., 2016, Essensial oil composition og pinus peuce Griseb. needles and twings from two national parks of Kosova. The Scientific World Journal, PMC49927553. BRUGGISSER, R., VON DAENIKEN, K., JUNDT, G., SCHAFFNER, W., 2002, Interference of plant extract phytoestrogens and antioxidants with MMT tetrazolium assay. Planta Med., 68(5), 445-448. CAN, G., KALAYCI, Ş, KAPLAN, R., 2006, Üç farklı kompozitin yapay tükürük ortamında flor salınım değerlerinin incelenmesi. Atatürk Üniv. Diş Hek. Fak. Derg., 33(1):33-38. DURMUS, M., YILMAZ, H.R., UZ, E., OZCELİK, N., 2008, The Effect of Caffeic Acid Phenethyl Ester (CAPE) Treatment on Levels of MDA, NO and Antioxidant Enzyme Activities in Retinas of Streptozotocin-Induced Diabetic Rats, Turk J Med Sci., 38 (6): 525-530 ICONOMOU, N., VALKANAS, G., BÜCHI, J., 1964, Composition of gum turpentines of Pinus halepensis and Pinus brutia grown in Greece [J]. J Chromatogr, 16: 29-33. İLHAMİ, G., 2006, Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology, 217(2-3):213–220. INCE, I., YESİL-CELİKTAS, O., KARABAY-YAVASOGLU, NU., ELGIN, G., 2009, Effects of Pinus brutia bark extract and Pycnogenols in arat model of carrageenan induced inflammation, Phytomedicine, 16 1101–1104. KIM, H.G., HAN, E.H., IM, J.H., LEE, E.J., JIN, S.W., 2015, Caffeic acid phenethyester inhibits 3-MC induced CYP1A1 expression through induction of hypoxio-inducible factor-1α. Biochem. Biophys. Research Comm., 465: 562-568. KIM, Y.M., JEONG, Y.K., WANG, M.H., LEE, W.Y., RHEE H.I., 2005, Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition. 21(6):756-61. KIM, Y.M., WANG, M.H., RHEE, H.I., 2004, A novel alpha-glucosidase inhibitor from pine bark. Carbohydr Res., 339(3):715-7. MAIMOONAA, A., NAEEMA, I., SADDİQEA, Z., JAMEELB, K., 2011, A review on biological, nutraceutical and clinical aspects of French maritime pine bark extract. J. Ethnopharm., 133: 261-277. MOSMANN,T., 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods., 65(1-2):55-63. OKTAR, S., AYDIN, M., YONDEN, M., ALCIN, E., İLHAN,S. etal., 2010, Effects of caffeic acid phenethyl ester on isoproterenol-induced myocardial infarction in rats. Anadolu Kardiyol Der., 10: 298-302. OLTHOF, M.R., HOLLMAN, P.C., KATAN, M.B., 2001, Chlorogenic acid and caffeic acid are absorbed in humans. J. Nutr., 131 (1): 66–71. OROIAN, M., ESCRICHE, I., 2015, Antioxidants: Characterization, natural sources, extraction and analysis. Food Research International, 74 10–36. RAJENDA PRASAD, N., KARTHIKEYAN, A., KARTHIKEYAN, S., REDDY, B.V., 2011, Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell line". Mol Cell Biochem, 349 (1-2): 11–9. RISS, T.L., MORAVEC,R.A., NILES A.L. et.al., 2013, Cell viability assays: Assay Guidance manuel., http://www.ncbi.nlm.nih.gov/books/NBK 144065/?report=reader) SAÇLI, S., AKALIN, E., 2001, Preliminary ethnobotanical study from Kaz dağı (Balıkesir/Çanakkale) I: Uses and vernacular names, İstanbul Eczacılık Fakültesi Mecmuası, 34 (2): 9. SATIL, F., SELVI, S., POLAT, R. 2011, Ethnic uses of pine resin production from Pinus brutia by native people on the Kazdağ Mountain (Mt. Ida) in Western Turkey [J]. J Food Agric Envir, 9 (3-4): 1059-1063. SCHILLER, G., GRUNWALD, C.1987, Cortex resin monoterpene composition in Pinus brutia provenances grown in Israel [J]. Biochem Syst Ecol., 15 (4): 389-394. ULUKANLI, Z.,KARABÖRKLÜ, S, BOZOK, F., ATES, B., ERDOGAN, S., et. al., 2014, Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus Brutia and Pinus pinea resin. Chinese Journal of Natural Medicines, 12(12): 901-910. YANG, N.Y, LIU, L., TAO, W.W., et al., 2010, Diterpenoids from Pinus massoniana resin and their cytotoxicity against A431 and A549 cells [J]. Phytochemistry, 71 (13): 1528-1533. YESIL-CELIKTAS, O., ISLETEN, M., VARDAR-SUKAN, F, CETIN, O., 2010, In vitro release kinetics of pinebark extract enriched orange juice and the shelf stability, British Food Journal, 112 ( 10):1063 - 1076 . YONEI, Y., MIZUNO, Y., KATAGIRI, E., 2004. Effects of cosmetics therapy using isoflavone and pine bark extract on the skin and QOL: a double-blind placebo-controlled trial. Anti-Aging Med. Res. 1, 48–58.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakoloji ve Eczacılık
Bölüm Makaleler
Yazarlar

Oytun ERTAŞ Bu kişi benim


Bengisu DÖNMEZ Bu kişi benim


Sibel KONYALIOGLU Bu kişi benim
Ege University, Faculty of Pharmacy, Department of Biochemistry, Bornova, İzmir
Türkiye


Özlem SÖĞÜT Bu kişi benim (Sorumlu Yazar)
Ege University, Faculty of Pharmacy, Department of Biochemistry, Bornova, İzmir

Yayımlanma Tarihi 31 Ocak 2018
Yayınlandığı Sayı Yıl 2018, Cilt 2, Sayı 1

Kaynak Göster

APA Ertaş, O. , Dönmez, B. , Konyalıoglu, S. & Söğüt, Ö. (2018). RESEARCH ON THE COMPOSITION AND CYTOTOXIC ACTIVITY OF PINUS BRUTIA GUM . Journal of Scientific Perspectives , 2 (1) , 39-48 . Retrieved from https://dergipark.org.tr/tr/pub/jsp/issue/36400/410518