GREEN SYNTHESIS of SILVER, ZINC, and CERIUM NANOPARTICLES USING THERMOPHILIC ANOXYBACILLUS SP. ST7 STRAIN and INVESTIGATION of THEIR VARIOUS BIOLOGICAL ACTIVITIES

Abstract

The antioxidant activities of AgNP, ZnNP and CeNP synthesized extracellular from thermophilic Anoxybacillus sp. ST7 were evaluated by DPPH scavenging activity and ferrous chelating activity. The highest DPPH and ferrous chelating activities of AgNP, CeNP, and ZnNP at 200 mg/L concentration were 93.59% and 88.08%, 73.04% and 78.25%, and 77.47% and 82.96%, respectively. Also, the nanoparticles demonstrated significant DNA cleavage activity. The antimicrobial capabilities of NPs were researched in micro-dilution methods and it was observed that Gram +ve bacteria were more susceptible to nanoparticles. The nanoparticles showed more effective microbial cell inhibition viability activity toward E. coli. Also, NPs showed important biofilm inhibition activity toward S. aureus and P. aeruginosa.

Keywords

• Thermophilic bacteria
• nanoparticles
• antioxidant
• antimicrobial activity
• biofilm inhibition
• microbial cell viability.

Thanks

I would like to thank Zelal Işık, Ömer Acer, and Erkan Yılmaz for their contribution to the study.

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