GREEN SYNTHESIS of SILVER, ZINC, and CERIUM NANOPARTICLES USING THERMOPHILIC ANOXYBACILLUS SP. ST7 STRAIN and INVESTIGATION of THEIR VARIOUS BIOLOGICAL ACTIVITIES

Year 2021, Issue: 047, 246 - 268, 31.12.2021

Serpil Gonca

Abstract

The antioxidant activities of AgNP, ZnNP and CeNP synthesized extracellular from thermophilic Anoxybacillus sp. ST7 were evaluated by DPPH scavenging activity and ferrous chelating activity. The highest DPPH and ferrous chelating activities of AgNP, CeNP, and ZnNP at 200 mg/L concentration were 93.59% and 88.08%, 73.04% and 78.25%, and 77.47% and 82.96%, respectively. Also, the nanoparticles demonstrated significant DNA cleavage activity. The antimicrobial capabilities of NPs were researched in micro-dilution methods and it was observed that Gram +ve bacteria were more susceptible to nanoparticles. The nanoparticles showed more effective microbial cell inhibition viability activity toward E. coli. Also, NPs showed important biofilm inhibition activity toward S. aureus and P. aeruginosa.

Keywords

Thermophilic bacteria, nanoparticles, antioxidant, antimicrobial activity, biofilm inhibition, microbial cell viability.

Supporting Institution

-

Project Number

-

Thanks

I would like to thank Zelal Işık, Ömer Acer, and Erkan Yılmaz for their contribution to the study.

References

• [1] Enderby, J. and Dowling, A., Nanoscience and nanotechnologies: Opportunities and Uncertainties, The Royal Society & The Royal Academy of Engineering Report, London, 2004.
• [2] Ramsden J., Nanotechnology: An introduction, (ISBN: 978-0-08-096447-8) Elsevier, 2011.
• [3] Lines M.G. (2008). Nanomaterials for Practical Functional Uses, Journal of Alloys and Compounds, 449, 242-245, 2008.
• [4] Scenihr, The appropriateness of Existing Methodologies to Assess the Potential Risks Associated with Engineered and Adventitious Products of Nanotechnologies, Committee Opinion, 58-59, 2006.
• [5] Ealias A.M., Saravanakumar M.P. A review on the classification, characterisation, synthesis of nanoparticles and their application, 14th ICSET-2017, IOP Conf. Series: Materials Science and Engineering 263 (2017) 032019 doi:10.1088/1757-899X/263/3/032019.
• [6] Baig, N., Kammakakam, I., Falath W. (2021). Nanomaterials: a review of synthesis methods, properties, recent progress, and challenges, Mater. Adv, 2, 1821–1871.
• [7] Duncan, T.V. (2011). Applications Of Nanotechnology In Food Packaging And Food Safety: Barrier Materials, Antimicrobials And Sensors. J Colloid Interface Sci 363(1): 1-24
• [8] Kumar, A., Chisti, Y., Banerjee, U. (2013). Synthesis of metallic nanoparticles using plant extracts: Biotechnology Advances (31) 346–356.
• [9] Nematollahi, F. (2015). Silver nanoparticles green synthesis using aqueous extract of Salvia limbata C. A. Mey. Research Paper. International Journal of Biosciences ISSN: 2220-6655 (Print), 2222-5234(Online) http://www.innspub.net Vol. 6, No. 2, p. 30-35.
• [10] Stephen Inbaraj B and Chen BH. (2020) An overview on recent in vivo biological application of cerium oxide nanoparticles. Asian J Pharm Sci. 15(5):558–75.
• [11] [11] Eka Putri G, Rilda Y, Syukri S, Labanni A, Arief S.(2021) Highly antimicrobial activity of cerium oxide nanoparticles synthesized using Moringa oleifera leaf extract by a rapid green precipitation method. J Mater Res Technol. 15 :2355–64.
• [12] Cheon JY, Kim SJ, Rhee YH, Kwon OH, Park WH. (2019). Shape-dependent antimicrobial activities of silver nanoparticles. Int J Nanomedicine. 14: 2773–80.
• [13] Estevez MB, Raffaelli S, Mitchell SG, Faccio R, Alborés S. (2020). Biofilm eradication using biogenic silver nanoparticles. Molecules. 25(9): 1-14.
• [14] Ruiz AL, Garcia CB, Gallón SN, Webster TJ. (2020). Novel silver-platinum nanoparticles for anticancer and antimicrobial applications. Int J Nanomedicine. 15: 169–79.
• [15] Mishra PK, Mishra H, Ekielski A, Talegaonkar S, Vaidya B. (2017). Zinc oxide nanoparticles: a promising nanomaterial for biomedical applications. Drug Discov Today. 22(12):1825–34.
• [16] Ağırtaş MS, Karatas C, Özdemir S. (2015). Synthesis of some metallophthalocyanines with dimethyl 5- (phenoxy) -isophthalate substituents and evaluation of their antioxidant-antibacterial activities. Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy. 135:20–4.
• [17] Dinis T.C.P, Madeira V.M.C, Almeida L.M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Arch. Biochem. Biophys. 315; 161-169.
• [18] Raghava S, Munnene Mbae K, Umesha S. (2021). Green synthesis of silver nanoparticles by Rivina humilis leaf extract to tackle growth of Brucella species and other perilous pathogens. Saudi J Biol Sci. 28(1):495–503.
• [19] Sharmilaa G, Thirumarimurugan M, Muthukumarana C. (2019) Green synthesis of ZnO nanoparticles using Tecoma castanifolia leaf extract: Characterization and evaluation of its antioxidant, bactericidal and anticancer activities. Microchemical Journal. 45: 578-587.
• [20] Shejawal KP, Randive DS, Bhinge SD, Bhutkar MA, Wadkar GH, Jadhav NR. (2020). Green synthesis of silver and iron nanoparticles of isolated proanthocyanidin: its characterization, antioxidant, antimicrobial, and cytotoxic activities against COLO320DM and HT29. J Genet Eng Biotechnol. 18(43), 1-11.
• [21] Farias IAP, Santos CCL, Xavier AL, Batista TM, Nascimento YM, Nunes JMFF, Silva PMF, Menezes-Júnior RA, Ferreira JM, Lima EO, Tavares JF, Sobral MV, Keyson D, Sampaio FC. (2021). Synthesis, physicochemical characterization, antifungal activity and toxicological features of cerium oxide nanoparticles. Arab J Chem. 14 (1), 102888.
• [22] Mahabadi AG, Mirzakhani A, Azizi A, Chavoshi S, Khaghani S.(2021). Extracts of Pelargonium hortorum: A natural and efficient fluid for fast and eco-friendly biosynthesis of CeO2 nanoparticles for antioxidant and photocatalytic applications. Inorg Chem Commun. 127, 108553.
• [23] Ameen F, Al-Homaidan AA, Al-Sabri A, Almansob A, AlNAdhari S. (2021). Anti-oxidant, anti-fungal and cytotoxic effects of silver nanoparticles synthesized using marine fungus Cladosporium halotolerans. Appl Nanosci. 1-9.
• [24] Gur T, Meydan I, Seckin H, Bekmezci M, Sen F. (2022). Green synthesis, characterization and bioactivity of biogenic zinc oxide nanoparticles. Environ Res. 204 (Part A):111897.
• [25] AlSalhi MS, Elangovan K, Ranjitsingh AJA, Murali P, Devanesan S. (2019). Synthesis of silver nanoparticles using plant derived 4-N-methyl benzoic acid and evaluation of antimicrobial, antioxidant and antitumor activity. Saudi J Biol Sci. 26 (5):970–8.
• [26] Soren S, Kumar S, Mishra S, Jena PK, Verma SK, Parhi P. (2018). Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method. Microb Pathog. 119 :145–51.
• [27] Gonca S, Arslan H, Isik Z, Özdemir S, Dizge N. (2021). The surface modification of ultrafiltration membrane with silver nanoparticles using Verbascum thapsus leaf extract using green synthesis phenomena. Surfaces and Interfaces. 26:101291.
• [28] De A, Das R, Kaur H, Jain P. (In Press). Synthesis, physicochemical investigations, DNA cleavage activity of biogenic zinc oxide nanoparticles and their interaction with Calf-Thymus DNA. Mater Today Proc. 1–5.
• [29] Al-Otibi F, Perveen K, Al-Saif NA, Alharbi RI, Bokhari NA, Albasher G, Al-Otaibi RM, Al-Mosa MA. (2021). Biosynthesis of silver nanoparticles using Malva parviflora and their antifungal activity. Saudi J Biol Sci. 28(4):2229–35.
• [30] Wang Q, Perez JM, Webster TJ. (2013). Inhibited growth of Pseudomonas aeruginosa by dextran- and polyacrylic acid-coated ceria nanoparticles. Int J Nanomedicine. 8:3395–9.
• [31] Bellio P, Luzi C, Mancini A, Cracchiolo S, Passacantando M, Di Pietro L, Perilli M, Amicosante G, Santucci S, Celenza G. (2018). Cerium oxide nanoparticles as potential antibiotic adjuvant. Effects of CeO2 nanoparticles on bacterial outer membrane permeability. Biochim Biophys Acta-Biomembr. 1860(11):2428–35.
• [32] Kumar KM, Mahendhiran M, Diaz MC, Hernandez-Como N, Hernandez-Eligio A, Torres-Torres G, Godavarthi S, Gomez LM. (2018). Green synthesis of Ce3+ rich CeO2 nanoparticles and its antimicrobial studies. Mater Lett. 214:15–9.
• [33] Zamanpour N, Mohammad A, Mashreghi M, Shahnavaz B. (2021). Bioorganic Chemistry Application of a marine luminescent Vibrio sp . B4L for biosynthesis of silver nanoparticles with unique characteristics, biochemical properties, antibacterial and antibiofilm activities. Bioorg Chem.; 114:1–12.
• [34] Ishwarya R, Vaseeharan B, Kalyani S, Banumathi B, Govindarajan M, Alharbi NS, Kadaikunnan S, Al-anbr MN, Khaled JM, Benelli G. (2018). Facile green synthesis of zinc oxide nanoparticles using Ulva lactuca sea weed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity, J. Photochem. Photobiol. B Biol. 178:249–258.
• [35] Altaf M, Manoharadas S, Zeyad MT. (2021) .Green synthesis of cerium oxide nanoparticles using Acorus calamus extract and their antibiofilm activity against bacterial pathogens. Microsc Res Tech. 84(8):1638–48.
• [36] Rajivgandhi G, Maruthupandy M, Muneeswaran T, Anand M, Quero F, Manoharan N, et al. (2019). Biosynthesized silver nanoparticles for inhibition of antibacterial resistance and biofilm formation of methicillin-resistant coagulase negative Staphylococci. Bioorg Chem. 2019;89 (March):103008.
• [37] Khan F, Lee JW, Pham DNT, Khan MM, Park SK, Shin IS, Kim YM. (2020). Antibiofilm Action of ZnO, SnO2 and CeO2 Nanoparticles Towards Grampositive Biofilm Forming Pathogenic Bacteria. Recent Pat Nanotechnol. 14(3):239-24.
• [38] Basumatari M, Devi RR, Gupta MK, Gupta SK, Raul PK, Chatterjee S, Dwivedi SK. (2021). Musa balbisiana Colla pseudostem biowaste mediated zinc oxide nanoparticles: Their antibiofilm and antibacterial potentiality. Curr Res Green Sustain Chem. 4 (December 2020):100048.