OPTIMIZATION of MICROBIAL CONSORTIA in the DEGRADATION of BIODIESEL EFFLUENT from JATROPHA CURCUS

Year 2022, Issue: 048, 14 - 24, 31.03.2022

Jude Osarumwense , Fidelis Okolafor

Abstract

The utilization of biodiesel produced from Jatropha curcus as renewable energy is relatively new area of research. The waste generated during biodiesel production may cause serious impact on the soil. The present study was aimed at optimizing microbial consortia in the degradation of biodiesel effluent (BDE). A portion of land (loamy soil) measuring 1.5 m by 1.5 m was polluted with BDE for 28 days. Standard microbiological and chemical methods were used to determine BDE utilizing organisms and physicochemical properties of the soil. The result of the total heterotrophic microbial counts from BDE polluted site at 0 to 28 days revealed significance p<0.0001, p<0.001 and p<0.01 for bacterial, fungal and yeast counts. Percentage occurrence of bacterial isolates from BDE polluted soil showed high values for Bacillus subtilis (28.95%), Pseudomonas aeruginosa (21.05%), Staphylococcus epidermidis (18.42%), Staphylococcus aureus (15.79%). Aspergillus niger (20.63%) and Saccharomyces kluyveri (14.29%) also recorded highest occurrence for fungi and yeast isolates respectively. Performance level for the growth rate of bacterial isolates in BDE showed highest against Staphylococcus epidermidis (OD 1.6 at day 4), Bacillus subtilis (OD 1.5 at day 6) and Pseudomonas aeruginosa (OD 1.4 at day 4). The result from this study revealed the effect of depth in the degradation capacity of consortia microorganisms in BDE polluted soil.

Keywords

Jatropha curcus, biodiesel effluent, microbial consortia, optimization, degradation

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