Kastamonu University Journal of Forestry Faculty 1303-2399 1309-4181 Kastamonu Üniversitesi 10.17475/kastorman.1845436 Forestry Sciences (Other) Ormancılık (Diğer) Antimicrobial Activity and Mode of Action of Phellinus hartigii Extract Investigated on Multidrug-Resistant Staphylococcus aureus Using Fourier-Transform Infrared Spectroscopy Çoklu İlaca Dirençli Staphylococcus aureus Üzerinde Phellinus hartigii Ekstraktının Antimikrobiyal Aktivitesi ve Etki Mekanizmasının Fourier Dönüşüm Infrared Spektroskopisi Kullanılarak İncelenmesi Akçay Kaan KASTAMONU ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR. Tekşen Kübra KASTAMONU ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR. Şenturan Umay Merve KASTAMONU ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR. Altınöz Eda KASTAMONU ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR. Özerkan Dilşad KASTAMONU UNIVERSITY, FACULTY OF ENGINEERING-ARCHITECTURE, DEPARTMENT OF GENETICS AND BIOENGINEERING, GENETICS AND BIOENGINEERING PR. Akata İlgaz ANKARA ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ Altuner Ergin Murat KASTAMONU ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR. 12 25 2025 25 3 409 421 01 15 2025 06 23 2025 Copyright © 2001, Kastamonu Üniversitesi Orman Fakültesi Dergisi 2001 Kastamonu Üniversitesi Orman Fakültesi Dergisi

Aim of study: Macrofungi have garnered attention as promising reservoirs for novel antibiotics. Phellinus hartigii is known to have antimicrobial properties against various microorganisms. This study aims to investigate the in vitro mode of action of the ethanol extract of P. hartigii (PH-EtOH) against multidrug-resistant (MDR) Staphylococcus aureus using Fourier-transform infrared (FTIR) spectroscopy.Material and method: The composition of the extract was determined by GC-MS analysis, and its antimicrobial activity was evaluated through MIC and MBC tests. The effect of the extract was further analysed using FTIR spectroscopy.Main results: The PH-EtOH extract demonstrated antibacterial activity with a MIC of 53 µg/mL and an MBC of 420 µg/mL. The major constituents of PH-EtOH were ethyl oleate, linoleic acid ethyl ester, and hexadecanoic acid ethyl ester. FTIR spectra showed changes mainly in proteins and nucleic acids.Research highlights: The results of this study provide a starting point for further research into the use of P. hartigii ethanol extract as an antimicrobial agent against MDR S. aureus.

Çalışmanın amacı: Makrofunguslar, yeni antibiyotikler için umut vadeden rezervuarlar olarak dikkat çekmektedir. Phellinus hartigii’nin çeşitli mikroorganizmalara karşı güçlü antimikrobiyal özellikler taşıdığı gösterilmiştir. Bu çalışma, P. hartigii'nin etanol ekstraktının (PH-EtOH) çoklu ilaca dirençli (MDR) Staphylococcus aureus'a karşı in vitro etki şekline ilişkin FTIR tabanlı kanıt sağlamayı amaçlamaktadır.Materyal ve yöntem: PH-EtOH'nin bileşimi GC-MS analizi kullanılarak belirlenmiş ve S. aureus'a karşı antimikrobiyal aktivitesi MİK ve MBK testleri ile değerlendirilmiştir. Ekstraktın etkisi FTIR spektroskopisi kullanılarak analiz edilmiştir.Temel sonuçlar: PH-EtOH ekstraktı, S. aureus'a üzerinde 53 µg/mL'lik bir MİK ve 420 µg/mL'lik bir MBK ile antibakteriyel aktivite göstermiştir. PH-EtOH'nin ana bileşenleri etil oleat, linoleik asit etil ester ve heksadekanoik asit etil ester olarak bulunmuştur. FTIR spektrumları, başlıca protein ve nükleik asitlerde değişiklikler göstermiştir.Araştırma vurguları: Bu çalışmanın sonuçları, P. hartigii etanol özütünün MDR S. aureus'a karşı antimikrobiyal ajan olarak kullanımına yönelik daha ileri araştırmalar için bir başlangıç noktası sağlamaktadır.

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