Kastamonu University Journal of Forestry Faculty 1303-2399 1309-4181 Kastamonu Üniversitesi 10.17475/kastorman.498413 Chemical Composition and Antimicrobial Activities of Cold-Pressed Oils Obtained From Nettle, Radish and Pomegranate Seeds Altuner Ergin Murat Çeter Talip Gür Mahmut Güney Kerim Kıran Bayram Akwıeten Hana Ealoma Soulman Sana İ. 12 28 2018 18 3 236 247 02 21 2018 06 28 2018 Copyright © 2001, Kastamonu Üniversitesi Orman Fakültesi Dergisi 2001 Kastamonu Üniversitesi Orman Fakültesi Dergisi

ÖzÇalışmanın amacı: Bu çalışmanın amacı ısırgan (Urtica dioica), turp (Raphanus sativus) ve nar (Punica granatum) tohumlarından eldeedilen soğuk sıkım yağların antimikrobiyal etkilerinin ortaya koymaktır.Materyal ve Yöntem: Bu tohumların yağları, Staphylococcus epidermidisDSMZ 20044, Staphylococcus aureusATCC 25923, Salmonella typhimurium SL1344, Salmonella kentucky, Salmonella infantis, Salmonella enteritidis, Pseudomonas fluorescens P1 ATCC 13075, Pseudomonas aeruginosa DSMZ 50071, Klebsiella pneumoniae, Escherichia coli ATCC 25922, Enterococcus faecium, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Candida albicans DSMZ 1386 ve Bacillus subtilis DSMZ 1971 gibi 15 mikroorganizmaya karşı disk difüzyonmetodu ve MİK testi kullanılarak, antimikrobiyal aktiviteleri açısındanaraştırılmıştır. Sonuçlar sefazolin, klindamisin, kloramfenikol,siprofloksasin, amoksisilin/klavulanik asit, sülfametoksazol/trimetoprim,seftriakson, gentamisin, ampisilin, sefalotin, sefuroksim ve vankomisin olmaküzere 11 standart antibiyotik ile karşılaştırılmıştır. Ekstraktlar ayrıca GC-MSkullanılarak kimyasal olarak analiz edilmiştir.Sonuçlar: Sonuç olarak, turp yağının en yüksek aktiviteyigöstererek bütün mikroorganizmalara, ısırgan yağının ise S. epidermidis hariçbütün mikroorganizmalara etkili olduğu gözlenmiştir. En düşük aktivite ise naryağında gözlenmiştir.Önemli vurgular: Çalışmanın sonuçları, açık bir şekilde, ısırganotu, turp ve nardan elde edilen yağların destekleyici sağlık ürünü olarak veilave çalışmalarla medikal amaçlı olarak da kullanılabileceğini ortayakoymaktadır.

Abstract Aim of study: The aim ofthis study is to put forward the antimicrobial activity of cold pressed oilsobtained from seeds of nettle (Urticadioica), radish (Raphanus sativus)and pomegranate (Punica granatum).Material andMethods: Oils ofthese seeds were analysed for their antibacterial and antifungal activities bythe disk diffusion and MIC tests against fifteen microorganisms, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus ATCC 25923, Salmonella typhimurium SL 1344, Salmonella kentucky, Salmonella infantis, Salmonella enteritidis, Pseudomonas fluorescens P1 ATCC 13075, Pseudomonas aeruginosa DSMZ 50071, Klebsiella pneumoniae, Escherichia coli ATCC 25922, Enterococcus faecium, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Candida albicans DSMZ 1386 and Bacillus subtilis DSMZ 1971. The resultswere compared against 11 standard antibiotics, which are cefazolin,clindamycin, chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid,sulfamethoxazole/trimethoprim, ceftriaxone, gentamicin, ampicillin, cephalothin,cefuroxime and vancomycin. The extracts were also chemically analysed by usingGC-MS.Main results: As a result, radish oil is observed to be activeagainst all microorganisms with the highest activity, where nettle oil isactive against all microorganisms except for S. epidermidis. The lowest activity was observed in pomegranateoil.Highlights: The results of the study clearly puts forward thatoils obtained from nettle, radish and pomegranate could have a possiblemedicinal use.

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