Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya
Öz
Abstract
Aim of study: The aim of the study was to isolate the fungi associated with root-knot nematodes and to determine their effect on its eggs hatching in the Green Mountain region of Libya.
Material and methods: Soil samples infected with root-knot nematode were collected from the some farms of the Green Mountain region in Libya. Sample collection was carried out during May 2007 within ten different areas. Two fields planted with tomato plants were chosen from the each area. Different types of culture media were used to isolate the fungus species. Fifteen fungi were isolated from the rhizosphere of infected plants. Then, the effect of some isolated and identified fungi on the rate of hatching of nematode eggs was tested.
Main results: Isolation results indicate that some of the isolated fungi were considered as parasites on the nematodes. In the study area, Arthrobotrys sp. had the highest percentage of occurrence (70%) and the least one was Penicillium sp. (12.25%). Three inoculum levels of 0.75, 1.5, 2×106 fungal propagules were tested and the highest hatching inhibition (90.5%) was recorded in Paecilomyces lilacinus and the lowest inhibition level (58.4%) was observed in Vertecillium chlamydosporium.
Research highlights: As a result of this study, more successful outcomes can be obtained during the biological control of root-knot nematodes.
Anahtar Kelimeler
Root-knot Nematode,Fungi isolation,Egg hatching inhibition,Green Mountain
Kaynakça
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