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Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey

Cilt: 19 Sayı: 1 27 Mart 2019
Ayten Dizkırıcı *, İsmail Acar , Ayşenur Kalmer , Yusuf Uzun
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Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey

Öz

Aim of Study: The specimen identified as Hebeloma subtortum based on both morphological and molecular characterizations. Area of Study: Samples were collected from Bingöl province and the study was conducted at the Department of Molecular Biology and Genetics in Van Yüzüncü Yıl University.
Material and Methods: Characters of pileus, stipe, lamellae and basidia, cystidia, spores were used as macroscopic and microscopic features, respectively. DNA sequences of two loci including the internal transcribed spacer (ITS) region and the large subunit (LSU) of nuclear ribosomal RNA gene were used to show the evolutionary relationship and taxonomic position of the species within Hebeloma genus. The DNA sequences of the above-mentioned regions of H. subtortum were compared to those of the same and different species of the genus downloaded from NCBI. Main results: In phylogenetic analyses, H. subtortum distinctly clustered with its representatives retrieved from NCBI with high bootstrap value. The ITS tree was more informative compared to LSU. Hebeloma subtortum closely grouped with H. mesophaeum in the ITS tree. Research highlights: Hebeloma subtortum has been described and illustrated as a new record from Turkey.

Anahtar Kelimeler

Hebeloma, ITS, mycogenetics, phylogeny

Kaynakça

  1. Aanen, D.K., Kuyper, T. W., Boekhout, T. & Hoekstra, R.F. (2000). Phylogenetic relationships in the genus Hebeloma based on ITS 1 and 2 sequences, with special emphasis on the Hebeloma crustuliniforme complex. -Mycologia 92, 269–281.
  2. Aanen, D.K., Kuyper, T.W. & Hoekstra, R.F. (2001). A widely distributed ITS polymorphism within a biological species of the ectomycorrhizal fungus Hebeloma velutipes. Mycological Research 105,284–290.
  3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1997). Basic local alignment search tool. Journal of Molecular Biology 215, 403-410.
  4. Beker, H.J., Eberhardt, U. & Vesterholt J. (2016). Fungi Europaei Vol. 14, Hebeloma (Fr.) P. Kumm. Edizioni Tecnografica. Boyle, H., Zimdars, B., Renker, C. & Buscot, F. (2006). A molecular phylogeny of Hebeloma species from Europe. –Mycological Research 110, 369–380.
  5. Demirel, R. (2016). Comparison of rDNA regions (ITS, LSU, and SSU) of some Aspergillus, Penicillium, and Talaromyces spp. Turkish Journal of Botany 40, 576-583. Doğan, H.H. & Kurt, F. (2016). New macrofungi records from Turkey and macrofungal diversity of Pozantı-Adana. Turkish Journal of Botany 40,209-217.
  6. Doyle, J.J. & Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19,11-15.
  7. Eberhardt, U., Beker, H.J. & Vesterholt, J. (2015a). Decrypting the Hebeloma crustuliniforme complex: European species of Hebeloma section Denudata subsection Denudata (Agaricales). Persoonia 35, 101–147.
  8. Eberhardt, U., Ronikier, A., Schütz, N. & Beker, H.J. (2015b). The genus Hebeloma in the alpine belt of the Carpathians including two new species. Mycologia 107 (6), 1285-1303.
  9. Güngör, H., Solak, M.H., Allı, H., Işıloğlu, M. & Kalmış, E. (2015). New records for Turkey and contributions to the macrofungal diversity of Isparta Province. Turkish Journal of Botany 39, 867-877.
  10. Mycobank. http://www.mycobank.org/quicksearch.aspx; accessed 25 February 2018.

Kaynak Göster

APA
Dizkırıcı, A., Acar, İ., Kalmer, A., & Uzun, Y. (2019). Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty, 19(1), 1-10. https://doi.org/10.17475/kastorman.543375
AMA
1.Dizkırıcı A, Acar İ, Kalmer A, Uzun Y. Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty. 2019;19(1):1-10. doi:10.17475/kastorman.543375
Chicago
Dizkırıcı, Ayten, İsmail Acar, Ayşenur Kalmer, ve Yusuf Uzun. 2019. “Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey”. Kastamonu University Journal of Forestry Faculty 19 (1): 1-10. https://doi.org/10.17475/kastorman.543375.
EndNote
Dizkırıcı A, Acar İ, Kalmer A, Uzun Y (01 Mart 2019) Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty 19 1 1–10.
IEEE
[1]A. Dizkırıcı, İ. Acar, A. Kalmer, ve Y. Uzun, “Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey”, Kastamonu University Journal of Forestry Faculty, c. 19, sy 1, ss. 1–10, Mar. 2019, doi: 10.17475/kastorman.543375.
ISNAD
Dizkırıcı, Ayten - Acar, İsmail - Kalmer, Ayşenur - Uzun, Yusuf. “Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey”. Kastamonu University Journal of Forestry Faculty 19/1 (01 Mart 2019): 1-10. https://doi.org/10.17475/kastorman.543375.
JAMA
1.Dizkırıcı A, Acar İ, Kalmer A, Uzun Y. Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty. 2019;19:1–10.
MLA
Dizkırıcı, Ayten, vd. “Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey”. Kastamonu University Journal of Forestry Faculty, c. 19, sy 1, Mart 2019, ss. 1-10, doi:10.17475/kastorman.543375.
Vancouver
1.Ayten Dizkırıcı, İsmail Acar, Ayşenur Kalmer, Yusuf Uzun. Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty. 01 Mart 2019;19(1):1-10. doi:10.17475/kastorman.543375