Preliminary Results of Wood Endophytes of Abies cilicica in Yenişarbademli in Isparta Province

Yıl 2012, Cilt: 12 Sayı: 3, 275 - 278, 01.09.2012

A. Lehtıjärvı H.t. Doğmuş Lehtıjärvı A.g. Aday Kaya F. Oskay

Öz

Anahtar Kelimeler

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Preliminary Results of Wood Endophytes of Abies cilicica in Yenişarbademli in Isparta Province

Öz

Fungal colonisation originating from endophytic thalli in wood of healthy Taurus fir (Abies cilicica) was studied. Bore cores were taken from living trees during field surveys. The bore cores were cut into 0.5 cm long fragments, washed with 70% (v/v) ethanol, flamed and placed into petri dishes containing malt extract, agar and streptomycin. The plates were incubated at 25°C for 4 weeks. The outgrowing mycelia were pure-cultured and identified based on amplification and sequencing of the internal transcribed spacer (ITS) region of their nuclear ribosomal DNA. Totally 12 different fungal species were isolated from Abies cilicica wood cores. Five of them belong to Basidiomycetes. The present study is the first investigation of endophytic wood fungi occurring in fir in Turkey using DNA-based methods. Therefore the study is expected to provide new information of the pioneering colonizers of wood tissues in stems of living Taurus firs

Anahtar Kelimeler

Abies cilicica, wood tissue, endophyte, Basidiomycetes

Kaynakça

• Arnold, A.E., 2007. Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biological Reviews 21:51–56.
• Arnold, A.E., 2008. Endophytic fungi: hidden components of tropical community ecology. In: Schnitzer S, Carson W (eds) Tropical forest community ecology. Blackwell Scientific, Inc, pp 254–271
• Arnold, A.E., Miadlikowska, J., Higgins, K.L., Sarvate, S.D., Gugger, P., Way, A., Hofstetter, V., Kauff, F., Lutzoni, F., 2009. A phylogenetic estimation of trophic transition networks for ascomycetous symbiotrophic fungal diversification? Syst Biol 58:224–239 are lichens cradles of
• Arhipova, N., Gaitnieks, T., Donis, J., Stenlid, J., and Vasaitis, R. 2011. Decay, yield loss and associated fungi in stands of grey alder (Alnus incana) in Latvia. Forestry, 84(4): 337–348. doi:10.1093/ forestry/cpr018.
• Barklund, P., Kowalski, T., 1996. Endophytic fungi in branches of Norway spruce with particular reference to Tryblidiopsis pinastri. Can J Bot 74:673–678
• Boddy, L., and Rayner, A. D. M. , 1983. Origins of decay in living deciduous trees: the role of moisture content and a re-appraisal of the expanded concept of tree decay. New Phytologist 94, 623-641.
• Chapela, I.H., 1989. Fungi in healthy ítems and branches of American beech and aspen: a comparative study. New Phytol 113:65–75
• Chapela, I.H., and Boddy, L., 1988. Fungal colonization of attached beech branches. I. Early stages of development of fungal communities. New Phytol 110:39–45
• Dix, N.J., and Webster, J., 1995. Fungal Ecology. Chapman and Hall, London.
• Eaton, R., 2000. A breakthrough for wood decay fungi. New Phytologist 146: 3-4.
• Fisher, P.J. and Petrini, O., 1990. A comparative study of fungal endophytes in xylem and bark of Alnus species in England and Switzerland. Mycol. Res. 94, 313–319.
• Highley, T.L., 1990. Laboratory studies on antagonism of Scytalidium lignicola to wood decay fungi. JournalMaterial und Organismen, Vol. 25 No. 3 pp. 181-192 Hutchison, L.J., 1999. Wood-inhabiting
• microfungi isolated from Populus tremuloides from
• Alberta and northeastern British Columbia. Can J Bot 77:898–905.
• Norden, B., Appelquist, T., Lindahl, B., Henningsson, M., 1999. Cubic rot fungi–corticoid fungi in highly brown rotted spruce stumps. Mycologia Helvetica 10, 13–24.
• Nordén, B., Ryberg, M., Gotmark, F., Olausson, B., 2004. Relative importance of coarse and fine woody debris for the diversity of wood-inhabiting fungi in temperate broadleaf forests. Biol Conserv 117:1–10.
• Oses, R., Valenzuela, S., Freer, J., Sanfuentes, E., Rodriguez, J., 2008. Fungal endophytes in xylem of healthy Chilean trees and their possible role in early wood decay. Fungal Divers 33:77–86.
• Pechmann, H.V., Aufsess, H.V., and Rehfuess, K.E., 1973. Ursachen und Ausmaß von Stammfäulen in Fichtenbeständen auf verschiedenen Standorten. Forstw. Cbl. 92(1): 68–89. doi:10.1007/BF02736033.
• Petrini, O., 1991. Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds.). Microbial ecology of leaves, pp. 179-197. Springer-Verlag, New York.
• Petrini, O., and Fisher, P.J., 1988. A comparative study of fungal endophytes in xylem and whole stem of Pinus sylvestris and Fagus sylvatica. Trans Br Mycol Soc 91:233–238
• Petrini, O., 1996. Ecological and physiological aspects of host specificity in endophytic fungi. In: Redlin, S.C., Carris, L.M. (Eds.), Endophytic Fungi in Grasses and Woody Plants, Systematics, Ecology and Evolution. APS Press, St. Paul, MN, pp. 87–100.
• Ragazzi, A., Moricca, S., Capretti, P., Dellavalle, I., Turco, E., 2003. Differences in composition of endophytic mycobiota in twigs and leaves of healthy and declining Quercus species in Italy. For Pathol 33:31–38.
• Rayner, A.D.M. and Boddy, L., 1988. Fungal Decomposition of Wood, its Biology and Ecology. John Wiley and Sons, Chichester, New York, Brisbane, Toronto, Singapore.
• Santamaría, O., Díez, J.J., 2005. Fungi in leaves, twigs and stem bark of Populus tremula from northern Spain. For Pathol 35:95–104
• White, T., Bruns, T., Lee, S., Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J. (Eds.) PCR Applications. Academic Press, New York. to Methods and