ktd

Kocatepe Tıp Dergisi

3061-9904

Afyonkarahisar Sağlık Bilimleri Üniversitesi

10.18229/kocatepetip.1340255

Cell Development, Proliferation and Death Signal Transduction

Hücre Gelişimi, Proliferasyon ve Ölümü Sinyal İletimi

EFFECT OF APIUM GRAVEOLENS EXTRACTS ON CASPASE-3, -8, -9 AND APAF-1 IN LNCaP CELLS

APIUM GRAVEOLENS EKSTRAKTLARININ LNCaP HÜCRELERİNDE KASPAZ-3, -8, -9 ve APAF-1 ÜZERİNE ETKİSİ

https://orcid.org/0000-0002-5353-3228

Koca

Halit Buğra

AFYONKARAHİSAR SAĞLIK BİLİMLERİ ÜNİVERSİTESİ

https://orcid.org/0000-0001-5510-9415

Köken

Tülay

YÜKSEK İHTİSAS ÜNİVERSİTESİ, TIP FAKÜLTESİ

https://orcid.org/0000-0002-6222-315X

Akan

Tülay

YÜKSEK İHTİSAS ÜNİVERSİTESİ, TIP FAKÜLTESİ

07 18 2024

25 3 300 306 08 09 2023 11 08 2023

1999

Kocatepe Tıp Dergisi

OBJECTIVE: In this study it was aimed to investigate the anti-cancer effect of ethanolic extracts of apium graveolens (celery) plant on cell viability, caspases involved in apoptosis and apoptosis protease activation factor-1 (Apaf-1) as inducer/suppressor in prostate cancer cell line LNCaP cells.MATERIAL AND METHODS: LNCaP cell line was used in our study. LNCaP cells were divided into 4 groups treated with ethanolic extracts of apium graveolens at increasing concentrations ranging from 0, 1500, 2000, 2500 μg/mL. The indicated extract concentrations were applied to LNCaP cells separately for 24 and 48 hours. Caspase-3, -8, -9 and Apaf-1 were measured by ELISA method from the cell lysate prepared at the end of the treatment. The results were expressed as ng/ml in proportion to the number of viable cells.RESULTS: Treatment of LNCaP prostate cancer cells with apium graveolens extracts for 24 and 48 hours did not cause a significant difference in caspase-3 and caspase-9 levels. However, caspase-8 levels were significantly increased at 2000 and 2500 μg/ml extract concentrations and Apaf-1 levels were significantly increased at 2500 μg/ml extract concentration in a dose- and time-dependent manner compared to the control groups at 24 and 48 hours.CONCLUSIONS: The results of this study show that ethanolic extracts of apium graveolens plant have anticancer and apoptotic properties by increasing caspase-8 and Apaf-1 levels in LNCaP prostate cancer cell line. Further in-vitro and in-vivo studies are needed to support our findings.

AMAÇ: Bu çalışmada, prostat kanseri hücre hattı LNCaP hücrelerinde, apium graveolens (kereviz) bitkisinin etanolik ekstraktlarının hücre canlılığı ve apoptoziste rol alan kaspazlar ve apoptoz proteaz aktivasyon faktörü-1 (Apaf-1) üzerine indükleyici/baskılayıcı olarak anti-kanser etkisinin araştırılması amaçlandı.GEREÇ VE YÖNTEM: Çalışmamızda LNCaP hücre hattı kullanıldı. LNCaP hücreleri; 0, 1500, 2000, 2500 μg/mL arasında değişen ve artan konsantrasyonlarda apium graveolens'in etanolik ekstraktları ile muamele edilen 4 gruba ayrıldı. Belirtilen ekstrakt konsantrasyonları LNCaP hücrelerine 24 ve 48 saat ayrı ayrı uygulandı. Uygulama bitiminde hazırlanan hücre lizatından ELISA yöntemiyle; Kaspaz-3, -8, -9 ve Apaf-1 ölçümü yapıldı. Sonuçlar canlı hücre sayılarıyla oranlanarak ng/ml olarak verildi.BULGULAR: LNCaP prostat kanser hücrelerinin apium graveolens ekstraktları ile 24 ve 48 saatliğine muamelesi kaspaz-3 ve kaspaz-9 düzeylerinde anlamlı bir farklılığa neden olmadı. Bununla birlikte; 24 ve 48 saatlik uygulamada, 2000 ve 2500 μg/ml ekstrakt konsantrasyonlarında kaspaz-8 değerleri kontrol gruplarına göre ve 2500 μg/ml ekstrakt konsantrasyonunda ise Apaf-1 düzeyi kontrol gruplarına göre doza ve zamana bağımlı olarak istatistiksel bakımdan anlamlı artış gösterdi.SONUÇ: Bu çalışmanın sonuçları, apium graveolens bitkisinin etanolik ekstraktlarının uygulandığı LNCaP prostat kanser hücre hattında kaspaz-8 ve Apaf-1 düzeylerini artırarak antikanser ve apoptotik özelliğe sahip olduğunu göstermektedir. Bulgularımızı desteklemek için daha ileri in-vitro ve in-vivo çalışmalara ihtiyaç vardır.

Prostat Kanseri Kaspazlar Bitki Ekstreleri Hücre kültürü

Prostate Cancer Caspases Plant extracts Cell culture

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

16.KARİYER.188

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