Integration of Dendritic Mesoporus Silica Nanoparticles with Enlarged Pores into Polyethylene Glycol Dimethacrylate Scaffold and In Vitro Investigations

Abstract

In this study, three-dimensional (3D) polyethylene glycol dimethacrylate (PEGDMA) hydrogel scaffolds were prepared and doped with dendritic mesoporous silica nanoparticle (dMSN) to support two different cell cultivation methods by cell encapsulation and cell seeding on the prepared scaffolds. During studies, the effect of dMSNs in modulating the mechanical and biological activity of scaffolds was investigated to aid cell viability. Hydrodynamic size, net surface charge, and morphology of dMSN were characterized by dynamic light scattering, zeta potential measurement, and scanning electron microscopy imaging, respectively. After the blending of dMSN into PEGDMA scaffolds at different concentrations, mechanical and physical changes of hydrogel scaffolds were investigated by employing compression tests and swelling analysis. Effect of dMSN presence in PEGDMA scaffold matrix for culture of murine fibroblast cells were evaluated by colorimetric viability analysis. Results demonstrated that the blending of dMSN is beneficial to fine tuning elastic moduli and swelling ratios of 3D hydrogel scaffolds. These findings are endorsed with the viability assays demonstrating that cells encapsulated within dMSN-PEGDMA showed different degrees of viability in relation to dMSN concentration while cells seeded on top of the scaffolds exhibited increased cell viability over incubation time. Taken together, these results suggested that dMSN could be employed as biomolecule carriers in hydrogel scaffold systems to alter desired properties and to regulate the biological activity.

Keywords

• Mesoporous silica
• Sol-gel
• Tissue scaffold
• Polymer

Project Number

2019-TYL-FEBE-0009

POLİETİLEN GLİKOL DİMETİLAKRİLAT DOKU İSKELELERİNİN DENTRİTİK GÖZENEKLERİ GENİŞLETİLMİŞ MEZOGÖZENEKLİ SİLİKA NANOPARÇACIKLAR İLE KATKILANDIRILMASI VE IN VITRO İNCELEMELERİ

Abstract

Bu çalışmada, üç boyutlu (3D) polietilen glikol dimetakrilat (PEGDMA) hidrojel doku iskeleleri hazırlanmış ve hazırlanan doku iskelelerine hücre ekimi doku iskelesi üzerine ve kapsülleme yapılarak iki farklı hücre ekimi metodunu desteklemesi için dentritik mezogözenekli silika nanoparçacık (dMSN) ile katkılandırılması gerçekleştirilmiştir. Yapılan incelemelerle, dMSN'lerin hücre canlılığına yardımcı olmak için yapı iskelelerinin mekanik ve biyolojik aktivitesini düzenleme kapasitesi araştırılmıştır. dMSN'lerin hidrodinamik boyutu, net yüzey yükü ve morfolojisi, sırasıyla dinamik ışık saçılımı, zeta potansiyeli ölçümü ve taramalı elektron mikroskobu görüntülemesi ile karakterize edilmiştir. dMSN'lerin farklı konsantrasyonlarda PEGDMA iskelelerine karıştırılmasından sonra, hidrojel iskelelerinin mekanik ve fiziksel değişiklikleri sıkıştırma testleri ve şişme analizi kullanılarak gerçekleştirilmiştir. Murin fibroblast hücrelerinin kültürü için PEGDMA iskele matrisinde dMSN varlığının etkisi, kolorimetrik canlılık analizi ile değerlendirilmiştir. Sonuçlar, dMSN katkılandırmasının, 3D PEGDMA iskelelerinin hassas elastik modülleri ve şişme oranları için faydalı olduğunu göstermiştir. Bu bulgular ışığında, dMSN-PEGDMA iskelesi içinde kapsüllenen hücrelerin, değişen dMSN miktarlarına bağlı olarak canlılık oranlarında değişiklik gözlemlenirken, iskelelerin üstüne ekilen hücreler inkübasyon süresi boyunca artan hücre canlılığı sergilemiştir. Birlikte ele alındığında, bu sonuçlar, dMSN'lerin, 3D doku iskelesinin özelliklerini ve biyolojik aktivitesini düzenlemek için hidrojel iskele sistemlerinde biyomolekül taşıyıcıları olarak kullanılabileceğini ileri sürmektedir.

Keywords

• Mezogözenekli silika
• Sol-jel
• Doku iskelesi
• Polimer

Supporting Institution

Izmir Katip Celebi University, Bilimsel Araştırmalar Proje Koordinatörlüğü Birimi

Project Number

2019-TYL-FEBE-0009

Thanks

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu'na (TÜBİTAK) çalışmalar sırasında Ayşenur Pamukçu'ya “2210/C Ulusal Yüksek Lisans/Master Burs Programı Bilim ve Teknolojide Öncelikli Alanlar” programı kapsamında maddi olarak destek verdiği için teşekkürlerimizi sunarız .

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