Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 2619-9149 Kahramanmaraş Sütçü İmam Üniversitesi 10.18016/ksutarimdoga.vi.1614168 Plant Biotechnology Bitki Biyoteknolojisi Efficacy of Exogenous Abscisic Acid on Cholinesterase Enzyme Activity and Phenolic Compound Variability in Hypericum perforatum calli Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği https://orcid.org/0000-0003-2005-1019 Önlü Şeyma MUŞ ALPARSLAN ÜNİVERSİTESİ 07 04 2025 28 4 1083 1094 01 06 2025 05 17 2025 Copyright © 1997, Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 1997 Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

The aim of this study was to investigate the effect of abscisic acid (ABA) on cholinesterase enzyme activities and bioactive components of in vitro calli of Hypericum perforatum. Calli were obtained from Linsmaier Skoog (LS)/B5 medium containing 0.5 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 indole butyric acid (IBA). Different abscisic acid (0.01 mg L-1, 0.05 mg L-1, and 0.1 mg L-1) applications were performed on the calli obtained in tissue culture and extracted in 80% methanol. Hispidulin, fumaric acid, acacetin, epicatechin, and naringenin compounds were analyzed in Liquid chromatography–high–resolution mass spectrometry (LC-HRMS). While epicatechin (1.09 mg L-1) was found at the highest 0.05 mg L-1 ABA in callus samples, fumaric acid (2.30 mg L-1) and hispidulin (0.78 mg L-1) compounds were detected in the highest containing of 0.1 mg L-1 ABA. Acacetin (0.10 mg L-1) was produced in only 0.01 mg L-1 ABA, whereas naringenin (0.01 mg L-1) was produced in medium containing 0.05 mg L-1 ABA. The strongest Acetylcholinesterase (AChE) (0.207±0.012) and Butyrylcholinesterase (BChE) (0.243±0.019) inhibition activity was shown the best with 0.1 mg L-1 ABA application. This study has shown that ABA can be used as an elicitor in callus culture of H. perforatum, and the amounts of related metabolites can be changed via elicitors

Bu çalışmanın amacı, absisik asitin (ABA) Hypericum perforatum'un in vitro kalluslarının kolinesteraz enzim aktiviteleri ve biyoaktif bileşenleri üzerine etkisini araştırmaktır. Kalluslar, 0.5 mg L-1 thidiazuron (TDZ) ve 0.5 mg L-1 indol bütirik asit (IBA) içeren Linsmaier Skoog (LS)/B5 besiyerinden elde edildi. Doku kültüründe elde edilen kalluslara farklı absisik asit (0,01 mg L-1, 0,05 mg L-1 ve 0,1 mg L-1) uygulamaları yapıldı ve %80'lik metanolde ekstrakte edildi. Hispidulin, fumarik asit, akasetin, epikateşin, naringenin bileşikleri Sıvı Kromatografisi-Yüksek Çözünürlüklü Kütle Spektrometrisi (LC-HRMS) ile analiz edildi. Kallus örneklerinde en yüksek epikateşin (1.09 mg L-1) 0.05 mg L-1 ABA'da bulunurken, fumarik asit (2.30 mg L-1) ve hispidulin (0.78 mg L-1) bileşikleri en yüksek 0.1 mg L-1 ABA içerenlerde tespit edildi. Sadece 0.01 mg L-1 ABA içeren ortamda akasetin (0.10 mg L-1) üretilirken, 0.05 mg L-1 ABA içeren besin ortamında naringenin (0.01 mg L-1) üretildi. En güçlü Asetilkolinesteraz (AchE) (0.207±0.012) ve Bütirilkolinesteraz BChE (0.243±0.019) inhibisyon aktivitesinin 0.1 mg L -1 ABA uygulamasıyla olduğu ortaya çıktı. Bu çalışma, ABA'nın H.perforatum'un kallus kültüründe uyarıcı olarak kullanılabileceğini ve ilgili metabolitlerin miktarlarının elisitör aracılığıyla değiştirilebileceğini göstermiştir.

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