kujinas Kırşehir Ahi Evran Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2979-9198 Kırşehir Ahi Evran Üniversitesi Plant Cell and Molecular Biology Bitki Hücresi ve Moleküler Biyoloji Dermatofitlerin İzolasyonu, Moleküler Tanımlanması ve Bununla İlişkili Bazı Çevresel Faktörlerin İncelenmesi https://orcid.org/0000-0001-9791-0112 Mushıb Murad Khamees KIRŞEHİR AHİ EVRAN ÜNİVERSİTESİ https://orcid.org/0000-0002-7659-6336 Ahmed Waleed Khalid Tikrit University https://orcid.org/0000-0001-7436-5828 Gaffaroğlu Muhammet KIRŞEHİR AHİ EVRAN ÜNİVERSİTESİ 06 30 2023 1 1 1 9 12 14 2022 01 04 2023 Copyright © 2023, Kırşehir Ahi Evran Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2023 Kırşehir Ahi Evran Üniversitesi Fen Bilimleri Enstitüsü Dergisi

Bu çalışmanın amacı, dermatofitlerin laboratuvarda fungal test yöntemleriyle izole edilmesi ve teşhis edilmesi, ortaya çıkan türlerin tanımlanması ve mevcut yerel izolatların Ulusal Biyoteknoloji Bilgi Merkezi (NCBI) tarafından sağlananlarla karşılaştırılmasıdır. Dermatofit izolatları, kolonilerinin makroskopik ve mikroskopik özellikleri incelenerek tanımlanmıştır. Teşhislerinin doğruluğundan emin olmak için, PCR tekniği kullanılarak elde edilen sonuçlar, primerlerin (ITS1, ITS4) incelenen mantarların genomlarını çoğalttığını ve çoğaltılan bantların (600 baz çifti) arasında değiştiğini göstermiştir. Bu çalışmadaki 1.2.3.4 numaralı izolatlar, izolatlarla benzerlik göstermektedir. Genbank'ta OP752127 numarasıyla kayıtlı Candida albicans. 5.6.7.8.9 numaralı izolatlar da OP752433 numaralı GenBank'ta kayıtlı Candida glabrata izolatları ile benzerlik göstermektedir. 10.11.12 numaralı türler OP712459 numaralı GenBank'ta kayıtlı Candida tropicalisizolatları ile benzerlik göstermektedir. 13.14.15 ve 16 numaralı izolatlar GenBank OP712621'de kayıtlı Trichophyton mentagrophytes izolatları ile benzerlik göstermektedir. 17 ve 18 numaralı izolatlar da Scopulariopsis brevicaulis ile benzerlik göstermektedir ve Genbank OP752128'de kayıtlıdır.

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