Erişkin ratlarda Zerdaçal (Curcuma longa L.) Takviyesinin Kalp Dokusundaki Oksidan/Antioksidan Etkilerinin İncelenmesi

Abstract

Bu çalışmada, erişkin ratlarda zerdaçal (kurkumin) takviyesinin kalp dokusundaki oksidatif stres ve antioksidan savunma sistemi üzerinde meydana getirdiği etkileri incelendi. Çalışmada 8 haftalık 16 adet rat kullanılmış ve bu ratlar iki gruba ayrılmıştır. İlk grup olan deney grubundaki ratlara (n=8) 28 gün süresince günde 300 mg/kg dozunda kurkumin (C1386; Sigma Chemical, St. Louis, MO) mısır yağı içinde çözdürülerek ağızdan gavaj yöntemi ile verildi. İkinci grup olan kontrol grubundaki ratlara ise portör etkisini yok etmek için kurkumin ile eşit miktarda mısır yağı verildi. Ötenazi gerçekleştirildikten sonra kalp dokuları alınarak total antioksidan kapasiteleri (TAS), total oksidan kapasiteleri (TOS), malondialdehit (MDA) ve glutatyon (GSH) seviyeleri analiz edildi. Kurkumin takviyesi GSH seviyelerini önemli ölçüde artırmıştır (P<0,05). Bununla birlikte, kurkumin takviyesi istatistiksel olarak anlamlı olamamasına rağmen MDA seviyelerini azaltmıştır (P>0,05). Total antioksidan kapasitesi (TAS) ve total oksidan kapasitesi (TOS) oranları istatistiksel olarak anlamlı bulundu. Kurkumin takviyeli diğer grupta MDA seviyeleri kontrol grubuna oranla azalma eğilimi göstermiş olup iki grup arasında anlamlı istatistiksel bir fark bulunmamıştır. Kurkumin takviyesi erişkin ratlarda kalp dokusunu oksidatif hasara karşı korumakta ve antioksidan savunma sistemini güçlendirmektedir.

Keywords

• Kurkumin
• antioksidan
• oksidatif stres
• kalp

Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats

Abstract

n present study, the effects of curcumin supplementation on oxidative stress and antioxidant defense system in heart tissue in adult rats were investigated. Sixteen rats (8-weeks-old) were selected and divided into two groups. The first group is the experimental group and these rats (n=8) were gavaged with curcumin, dissolved in corn oil, at a dose of 300 mg/kg per day for 28 days. The second group is the control group, rats in this group were given curcumin and equal amount of corn oil to eliminate the porter effect. Euthanasia was performed and total antioxidant capacity (TAS), total oxidant capacity (TOS), malondialdehyde (MDA) and glutathione (GSH) levels were analyzed from heart tissues. Curcumin supplementation resulted in significantly increased GSH levels(P<0,05). However, curcumin supplementation decreased MDA levels although it was not statistically significant (P>0.05). The total antioxidant capacity (TAS) and total oxidant capacity (TOS) ratios were found to be statistically significant. In the other group supplemented with curcumin, MDA levels tended to decrease compared to the control group, and there was no statistically significant difference between the two groups. Curcumin supplementation protects the heart tissue against oxidative damage and strengthens the antioxidant defense system in adult rats.

Keywords

• Curcumin
• antioxidant
• oxidative stress
• heart

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