Histological demonstration of changes in connective tissue components in uterine tissue undergoing decidualization on days 4th, 5th, and 8th days of mouse pregnancy

Year 2023, , 293 - 299, 31.12.2023

Kübra Özcelikörs , Seval Türk , Esma Kırımlıoğlu

https://doi.org/10.24998/maeusabed.1334612

Abstract

This study examined the changes in connective tissue elements of the uterine undergoing decidualization on the 4th, 5th, and 8th days of pregnancy by histochemical methods. The study divided 6-8 weeks-old Balb/c mice into four groups non-pregnant estrous phase, 4th, 5th, and 8th-day pregnancy models. 5µm thick sections were taken from paraffin blocks obtained from uterine tissues. Samples were stained with Hematoxylin&Eosin, Mallory Azan, Orsein, and Periodic Acid Schiff stains. As a result of the staining, the uterine tissue in the non-pregnant estrus phase showed standard histological structure. On the 4th day of pregnancy, the amount of intensely stained collagen and elastic fiber decreased on the 5th day of gestation; On the 8th day of pregnancy, it was determined that the density of the fibers increased again. As a result, both increased collagen and elastic fibers for its placement to an elastic-solid uterine tissue and increased carbohydrates for its nutrient needs and immune privilege were demonstrated in the uterus for the 4th-day embryo. The decrease in connective tissue elements with the acceleration of decidualization on the 5th and increased collagen and elastic fibers in the myometrium and the PAS + NK cells in the endometrium on the 8th-day was noted.

Keywords

pregnancy, decidualization, connective tissue, histochemistry

Project Number

1919B012104121

References

• Abrahamsohn, P.A., 1983. Ultrastructural study of the mouse antimesometrial decidua. Anatomy and Embryology 166(2), 263-274.
• Alberto-Rincon, M.C., Zorn, T.M., Abrahamsohn, P.A., 1989. Diameter increase of collagen fibrils of the mouse endometrium during decidualization. The American Journal of Anatomy 186(4), 417-429.
• Croy, A., Yamada, A.T., DeMayo, F.J., Adamson, S.L., 2014. The Guide Investigation of Mouse Pregnancy. Elsevier, pp. 1-19.
• Das, S.K., 2010. Regional development of uterine decidualization: molecular signaling by Hoxa-10. Molecular Reproduction& Development 77, 387-396.
• Dimitriadis, E., White, C.A., Jones, R.L., et al. 2005. Cytokines, chemokines and growth factors in endometrium related to implantation. Human Reproductive Update 11(6), 613-630.
• Kierszenbaum, A.L., Tres, L.L., 2021. Histology and Cell Biology An Introduction to Pathology.4th ed. New York, NY, Elsevier.
• Koç, S., 2019. Gebeliğin Farklı Dönemlerindeki Fare Plasentasında Histolojik Ve Histokimyasal Değişiklikler İle Igg Dağılımının ve Yoğunluğunun Araştırılması. Histology And Embriyology (Veterinary) Program. Instutie Of Health Sciences: Aydın Adnan Menderes University Aydın.
• Mescher, A.L., 2018. Junqueira's Basic Histology Text&Atlas. 15th ed. US: Mc Graw Hill Education.
• Mutluay, D., 2015. Examination in the light microscopical level of the changes occurring in the rat uterus tissue during preimplantation. MAKÜ Saglık Bilimleri Enstitütü Dergisi 3(2), 43-53.
• Ng, S.W., Norwitz, G.A., Pavlicev, M., et al. 2020. Endometrial Decidualization: The Primary Driver of Pregnancy Health. International Journal of Moleculaar Sciences 21(11).
• Ramathal, C.Y., Bagchi, I.C., Taylor, R.N., et al. 2010. Endometrial decidualization: of mice and men. Seminars in Reproductive Medicine 28(1), 17-26.
• Spiess, K., Teodoro, W.R., Zorn, T.M. 2007. Distribution of collagen types I, III, and V in pregnant mouse endometrium. Connective Tissue Research 48(2), 99-108.
• Stumm, C.L., Zorn, T.M., 2007. Changes in fibrillin-1 in the endometrium during the early stages of pregnancy in mice. Cells Tissues Organs 185, 258-268.
• Stewart, I., Peel, S., 1980. Granulated metrial gland cells at implantation sites of the pregnant mouse uterus. Anatomy and Embryology 160(2), 227-238.
• Sur, E., Öznurlu, Y., Özaydin, T., Çelik, İ., Aydin, İ., Kadiralieva, N., 2015. Gebe farelerde desidua bazalis dokusundaki pas-pozitif uterus doğal katil hücrelerinin dağılımı. Kafkas Universitesi Veteriner Fakültesi Dergisi 21(3), 4005-4011.
• Teodoro, W.R., Witzel, S.S., Velosa, A.P., et al. 2003. Increase of interstitial collagen in the mouse endometrium during decidualization. Connective Tissue Research 44(2), 96-103.
• Wang, C., Zhao, M., Zhang, W.Q., et al. 2020. Comparative analysis of mouse decidualization models at the molecular level. Genes 11, 1-12.