Prevalence and Pattern of Antibiotic Susceptibility of Gram-Negative Bacteria Isolated from Pediatric Blood Culture

Abstract

It was aimed to evaluate the antibiotic susceptibilities of Gram-negative bacteria isolated from blood samples taken from pediatric patients. The samples were evaluated with the Bactec 9120 automation system bacteria were identified by the Gram-Negative ID panel using the Vitek 2 Compact (Biomerieux France) device performed with the AST N090 panel, and the results were evaluated according to The Clinical and Laboratory Standards Institute (CLSI) recommendations. Antibiotic resistance results: Ceftriaxone was the most resistant antibiotic (71.4%). Salmonella spp. it was mostly resistant to levofloxacin and ciprofloxacin (100%). While E. coli strains were the most resistant to ticarcillin and piperacillin (71.4%), the most effective antibiotics against this bacterium were imipenem and meropenem (100%). Resistance rates to all tested antibiotics were significantly higher in ESBL-producing Klebsiella spp and E coli strains than in non-ESBL-producing strains (p<0.05). Considering this information, it is vital to evaluate the current resistance profiles in the application of empirical treatment in premature and newborn patients.

Keywords

• Child
• Blood Culture
• Gram Negative
• Antibiotic Resistance
• Vitek 2

Pediatrik Kan Kültüründen İzole Edilen Gram Negatif Bakterilerin Prevalansı ve Antibiyotik Duyarlılık Paterni

Abstract

Pediatrik hastalardan alınan kan örneklerinden izole edilen Gram negatif bakterilerin antibiyotik duyarlılıklarının değerlendirilmesi amaçlandı. Örnekler Bactec 9120 otomasyon sistemi ile değerlendirildi. AST N090 paneli ile gerçekleştirilen Vitek 2 Compact (Biomerieux Fransa) cihazı kullanılarak Gram-Negatif ID paneli ile bakteriler tanımlandı ve sonuçlar Klinik ve Laboratuvar Standartları Enstitüsü (CLSI) tavsiyelerine göre değerlendirildi. Antibiyotik direnci sonuçları: Seftriakson en dirençli antibiyotik (%71,4) oldu. Salmonella spp. çoğunlukla levofloksasin ve siprofloksasine (%100) dirençliydi. E. coli suşları tikarsilin ve piperasilin'e en dirençli (%71,4) iken, bu bakteriye karşı en etkili antibiyotikler imipenem ve meropenem (%100) oldu. Test edilen tüm antibiyotiklere karşı direnç oranları, ESBL üreten Klebsiella spp ve E coli suşlarında, ESBL üretmeyen suşlara göre anlamlı derecede yüksekti (p<0,05). Bu bilgiler göz önüne alındığında prematüre ve yenidoğan hastalarda ampirik tedavinin uygulanmasında mevcut direnç profillerinin değerlendirilmesi hayati önem taşımaktadır.

Keywords

• Çocuk
• Kan Kültürü
• Gram Negatif
• Antibiyotik Direnci
• Vitek 2

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