Felin İnfeksiyöz Peritonitli Kedilerde Adenozin Deaminaz ve C-Reaktif Protein Düzeylerinin Araştırılması

Abstract

Investigations of Adenosine Deaminase and C-reactive Protein in Cats with Feline Infectious Peritonitis

Abstract

Abstract: The aims of the present study were to evaluate T-cell-mediated immune response and acute phase response by measuring serum adenosine deaminase (ADA-1), C reactive protein (CRP) and albumin (A) levels in cats with feline infectious peritonitis (FIP). In the study, 20 cats with FIP and 10 clinically healthy cats were used. In addition to that, cats with FIP were grouped as dry form (n=10) and wet form (n=10) of FIP according to clinical, radiographic and necropsy findings. In the study, serum and peritoneal effusion concentrations of ADA-1 and CRP, were determined by using cat-specific ELISA kits. Serum concentrations of total protein (TP), albumin and globulin were analysed by photometric methods. In the study, both serum and peritoneal effusion concentrations of ADA-1 (p<0.001) and CRP (p<0.001) were significantly higher in cats with FIP than control cats. A high positive correlation was obtained between serum concentrations of ADA-1 and CRP in cats with FIP (r=0.62, p<0.001). Significant increases in TP (p<0.01) and globulin (p<0.01) levels and decreases in A (p<0.01) values and A/G ratio (p<0.01) were obtained in cats with FIP compared to those of control group. Serum TP (p<0.05) and G (p<0.05) levels were significantly higher, whereas ADA-1 activitiy (p<0.01) was lower in cats with dry form than in cats with wet form of FIP. As a conclusion, elevated ADA-1 shows T-cell activation, and increased CRP and decreased albumin levels indicate the development of acute phase response in cats with FIP. It is suggestive that both serum and peritoneal effusion samples can be used to determine ADA-1 and CRP, and they can be useful biomarkers for evaluating T-cell-mediated immune responses, inflammation and possibly organ damages in cats with FIP. Keywords: Adenosine Deaminase-1 (ADA-1), Cat, C-Reactive Protein (CRP), Feline Infectious Peritonitis (FIP). Öz: Çalışmada felin infeksiyöz peritonitli (FİP) kedilerde serum adenozin deaminaz-1 (ADA-1), C-reaktif protein (CRP) ve albümin (A) seviyelerini ölçerek T-lenfosit aracılı immun yanıt ve akut faz yanıtın değerlendirilmesi amaçlanmıştır. Çalışmada 20 FİP’li ve 10 adet klinik olarak sağlıklı kedi kullanıldı. Ayrıca kediler klinik, röntgen ve nekropsi bulguları ışığında yaş form (n=10) ve kuru form (n=10) FİP’li olarak iki gruba ayrıldı. Kedilerin serum ADA-1 ve CRP düzeyleri kedi spesifik ELISA testleri kullanılarak belirlendi. Serum total protein (TP) ve albümin düzeyleri ise fotometrik metodla saptandı. Çalışmada FİP’li kedilerin hem serum hem de peritoneal efuzyonlarındaki ADA-1 (p<0,001) ve CRP (p<0,001) düzeyleri sağlıklı kedilerin düzeylerine göre istatistiksel olarak anlamlı düzeyde yüksek olduğu belirlendi. FİP’li kedilerin serum ADA-1 konsantrasyonu ile CRP konsantrasyonları arasında yüksek düşeyde pozitif korelasyonun olduğu belirlendi (r=0,62, p<0,001). Ayrıca FİP’li kedilerin serum TP (p<0,01) ve globülin (G) (p<0,01) düzeylerinde anlamlı artışlar belirlenirken albümin (p<0,01) düzeyleri ve albümin globülin oranlarında (A/G) ise sağlıklı kedilere göre anlamlı düzeyde düşüşlerin olduğu saptandı. Kuru form FİP’li kedilerin serum TP (p<0,05), ve G (p<0,05) düzeyleri yaş form FİP’li kedilerinkiyle karşılaştırıldığında anlamlı düzeyde yüksek iken ADA-1 p<0,01) aktivitesinin ise anlamlı düzeyde düşük olduğu saptandı. Sonuç olarak, FİP’li kedilerde artan serum ADA-1 aktivitesi T-lenfosit aktivasyonunu ve artan CRP ile düşen albümin ise akut faz yanıtın geliştiğini ortaya koymaktadır. FİP’li kedilerde hem serum hem de peritoneal efüzyonların ADA-1 ve CRP düzeylerinin ölçümünde kullanılabileceği ve bu parametrelerin FİP’li kedilerde T-lenfosit aracılı immun yanıt, yangı ve olası organ hasarlarının değerlendirilmesinde yararlı biyomarkırlar olduğu düşünülmektedir. Anahtar Kelimeler: Adenozin Deaminaz-1 (ADA-1), C-Reaktif Protein (CRP) Felin İnfeksiyöz Peritonitis (FIP), Kedi.

Keywords

• Adenosine Deaminase-1 (ADA-1)
• Cat
• C-Reactive Protein (CRP),
• Feline Infectious Peritonitis (FIP).

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