Research Article
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Year 2021, , 1 - 6, 30.04.2021
https://doi.org/10.24998/maeusabed.869812

Abstract

References

  • Ahn, Y.J., Lee, C.O., Kweon, J.H., Ahn, J.W., Park, J.H., 1998. Growth‐inhibitory effects of Galla Rhois‐derived tannins on intestinal bacteria. Journal of Applied Microbiology 84(3), 439-443.
  • Balaban Ucar, M., Ucar, G., Pizzi, A., Gonultas, O., 2013. Characterization of Pinus brutia bark tannin by MALDI-TOF MS and 13C NMR. Industrial Crops and Products 49, 697-704.
  • Bäumler, A.J., Sperandio, V., 2016. Interactions between the microbiota and pathogenic bacteria in the gut. Nature 535(7610), 85-93.
  • Canny G.O., McCormick B.A., 2008. Bacteria in the intestine, helpful residents or enemies from within? Infection and Immunity 76, 3360-3373.
  • CLSI (Clinical and Laboratory Standards Institute), 2016. M100-S26, Performance standards for antimicrobial susceptibility testing. 26th Informational Supplement, Wayne, PA, CLSI.
  • Das, A., Datta, S., Mukherjee, S., Bose, S., Ghosh, S., Dhar, P., 2015. Evaluation of antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and lignans. LWT - Food Science and Technology 61, 244-250.
  • Demirtaş, A., 2020. Influence of Pinus brutia bark extract containing phenolic compounds on some commensal and pathogenic bacteria from the intestinal microflora. Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi 5(2), 34-39.
  • Demirtas, A., Ozturk, H., Sudagidan, M., Keyvan, E., Yavuz, O., Yıldız-Gulay, Y., Musa, S.A.A., (2019). Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec). Anaerobe 55, 83-92.
  • Goker, G., Demirtas, A., 2020. Preliminary study on stimulatory and inhibitory effects of aldehydes from the green leaf volatiles family on beneficial and pathogenic bacteria from the intestine. Medycyna Weterynaryjna 76(3), 170-175.
  • Hmamouchi, M., Hamamouchi, J., Zouhdi, M., Bessiere, J.M., 2001. Chemical and antimicrobial properties of essential oils of five Moroccan Pinaceae. Journal of Essential Oil Research 13(4), 298-302.
  • Hobson, P.N., 1969. Rumen Bacteria. In: Methods in Microbiology. London and New York, Academic Press, pp. 133-149.
  • Hwang, Y.H., Lee, H.S., 2002. Antibacterial activity of Pinus densiflora leaf-derived components toward human intestinal bacteria. Journal of Microbiology and Biotechnology 12(4), 610-616.
  • Kaundun, S.S., Fady, B., Lebreton, P., 1997. Genetic differences between Pinus halepensis, Pinus brutia and Pinus eldarica based on needle flavonoids. Biochemical Systematics and Ecology 25(6), 553-562.
  • Keeratirathawat, S., Nair, J., Levitan, J., 2013. The antibacterial efficacy of pine oils on pathogens commonly found in biosolids applied to pine plantations. International Journal of Environment and Waste Management 12(2), 146-153.
  • Ko, H.H, Lareu, R.R., Dix, B.R., Hughes, J.D., 2018. In vitro antibacterial effects of statins against bacterial pathogens causing skin infections. European Journal of Clinical Microbiology 37, 1125-1135.
  • Ørskov F, Ørskov I., 1992. Escherichia coli serotyping and disease in man and animals. Canadian Journal of Microbiology 38(7), 699-704.
  • Patra A.K., Stiverson J., Yu Z., 2012. Effects of quillaja and yucca saponins on communities and select populations of rumen bacteria and archaea, and fermentation in vitro. Journal of Applied Microbiology 113, 1329-1340.
  • Rajkovic A., 2014. Microbial toxins and low level of foodborne exposure. Trends in Food Science & Technology 38(2), 149-57.
  • Shang, F.M., Liu, H.L., 2018. Fusobacterium nucleatum and colorectal cancer: A review. World Journal of Gastrointestinal Oncology 10(3), 71-81.
  • Üner, B., Karaman, İ., Tanrıverdi, H., Özdemir, D., 2011. Determination of lignin and extractive content of Turkish Pine (Pinus brutia Ten.) trees using near infrared spectroscopy and multivariate calibration. Wood Science and Technology 45(1), 121-134.
  • Yener, H.O., Saygideger, S.D., Sarikurkcu, C. Yumrutas, O., 2014. Evaluation of antioxidant activities of essential oils and methanol extracts of pinus species. Journal of Essential Oil Bearing Plants 17(2), 295-302.
  • Zeng, W.C., He, Q., Sun, Q., Zhong, K., Gao, H., 2012. Antibacterial activity of water-soluble extract from pine needles of Cedrus deodara. International Journal of Food Microbiology 153(1-2), 78-84.

Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine

Year 2021, , 1 - 6, 30.04.2021
https://doi.org/10.24998/maeusabed.869812

Abstract

Plant extracts have the potential to be safe alternatives to antibiotics that disrupt the gut flora. The aim of the present study was to assess the susceptibility of some gut bacteria to the extract from needles of Turkish pine (Pinus brutia Ten.) using microdilution method in an anaerobic chamber. Turkish pine needle extract promoted the growth of Bifidobacterium bifidum, Bifidobacterium infantis, and Lactobacillus acidophilus from gut commensals at 0.2-6.25 mg/mL, 0.4-6.25 mg/mL, and 0.4-1.6 mg/mL dose ranges, respectively (P<0.05). However, the extract had a potential inhibitory activity on Bifidobacterium species starting from 12.5 mg/mL, on L. acidophilus starting from 6.25 mg/mL, and L. casei starting from 3.13 mg/mL concentrations (P<0.05). Minimal inhibitor concentration (MIC) was 25 mg/mL for all commensal species. Turkish pine needle extract also showed a potential inhibitory activity against gut pathogens Escherichia coli and Clostridium perfringens from 0.4 mg/mL and against Staphylococcus aureus and Fusobacterium nucleatum from 0.8 mg/mL dose (P<0.05). The MICs were 6.25, 12.5, 25, and 50 mg/mL for S. aureus, F. nucleatum, E. coli, and C. perfringens, respectively. It was concluded that using the Turkish pine needle extract in a dose range of 0.2-12.5 mg/mL, where it protected most of the commensal bacteria and was toxic against some of the pathogens, might produce desirable impacts in the gut.

References

  • Ahn, Y.J., Lee, C.O., Kweon, J.H., Ahn, J.W., Park, J.H., 1998. Growth‐inhibitory effects of Galla Rhois‐derived tannins on intestinal bacteria. Journal of Applied Microbiology 84(3), 439-443.
  • Balaban Ucar, M., Ucar, G., Pizzi, A., Gonultas, O., 2013. Characterization of Pinus brutia bark tannin by MALDI-TOF MS and 13C NMR. Industrial Crops and Products 49, 697-704.
  • Bäumler, A.J., Sperandio, V., 2016. Interactions between the microbiota and pathogenic bacteria in the gut. Nature 535(7610), 85-93.
  • Canny G.O., McCormick B.A., 2008. Bacteria in the intestine, helpful residents or enemies from within? Infection and Immunity 76, 3360-3373.
  • CLSI (Clinical and Laboratory Standards Institute), 2016. M100-S26, Performance standards for antimicrobial susceptibility testing. 26th Informational Supplement, Wayne, PA, CLSI.
  • Das, A., Datta, S., Mukherjee, S., Bose, S., Ghosh, S., Dhar, P., 2015. Evaluation of antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and lignans. LWT - Food Science and Technology 61, 244-250.
  • Demirtaş, A., 2020. Influence of Pinus brutia bark extract containing phenolic compounds on some commensal and pathogenic bacteria from the intestinal microflora. Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi 5(2), 34-39.
  • Demirtas, A., Ozturk, H., Sudagidan, M., Keyvan, E., Yavuz, O., Yıldız-Gulay, Y., Musa, S.A.A., (2019). Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec). Anaerobe 55, 83-92.
  • Goker, G., Demirtas, A., 2020. Preliminary study on stimulatory and inhibitory effects of aldehydes from the green leaf volatiles family on beneficial and pathogenic bacteria from the intestine. Medycyna Weterynaryjna 76(3), 170-175.
  • Hmamouchi, M., Hamamouchi, J., Zouhdi, M., Bessiere, J.M., 2001. Chemical and antimicrobial properties of essential oils of five Moroccan Pinaceae. Journal of Essential Oil Research 13(4), 298-302.
  • Hobson, P.N., 1969. Rumen Bacteria. In: Methods in Microbiology. London and New York, Academic Press, pp. 133-149.
  • Hwang, Y.H., Lee, H.S., 2002. Antibacterial activity of Pinus densiflora leaf-derived components toward human intestinal bacteria. Journal of Microbiology and Biotechnology 12(4), 610-616.
  • Kaundun, S.S., Fady, B., Lebreton, P., 1997. Genetic differences between Pinus halepensis, Pinus brutia and Pinus eldarica based on needle flavonoids. Biochemical Systematics and Ecology 25(6), 553-562.
  • Keeratirathawat, S., Nair, J., Levitan, J., 2013. The antibacterial efficacy of pine oils on pathogens commonly found in biosolids applied to pine plantations. International Journal of Environment and Waste Management 12(2), 146-153.
  • Ko, H.H, Lareu, R.R., Dix, B.R., Hughes, J.D., 2018. In vitro antibacterial effects of statins against bacterial pathogens causing skin infections. European Journal of Clinical Microbiology 37, 1125-1135.
  • Ørskov F, Ørskov I., 1992. Escherichia coli serotyping and disease in man and animals. Canadian Journal of Microbiology 38(7), 699-704.
  • Patra A.K., Stiverson J., Yu Z., 2012. Effects of quillaja and yucca saponins on communities and select populations of rumen bacteria and archaea, and fermentation in vitro. Journal of Applied Microbiology 113, 1329-1340.
  • Rajkovic A., 2014. Microbial toxins and low level of foodborne exposure. Trends in Food Science & Technology 38(2), 149-57.
  • Shang, F.M., Liu, H.L., 2018. Fusobacterium nucleatum and colorectal cancer: A review. World Journal of Gastrointestinal Oncology 10(3), 71-81.
  • Üner, B., Karaman, İ., Tanrıverdi, H., Özdemir, D., 2011. Determination of lignin and extractive content of Turkish Pine (Pinus brutia Ten.) trees using near infrared spectroscopy and multivariate calibration. Wood Science and Technology 45(1), 121-134.
  • Yener, H.O., Saygideger, S.D., Sarikurkcu, C. Yumrutas, O., 2014. Evaluation of antioxidant activities of essential oils and methanol extracts of pinus species. Journal of Essential Oil Bearing Plants 17(2), 295-302.
  • Zeng, W.C., He, Q., Sun, Q., Zhong, K., Gao, H., 2012. Antibacterial activity of water-soluble extract from pine needles of Cedrus deodara. International Journal of Food Microbiology 153(1-2), 78-84.
There are 22 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Ahu Demirtaş 0000-0003-2942-6243

Publication Date April 30, 2021
Submission Date January 28, 2021
Published in Issue Year 2021

Cite

APA Demirtaş, A. (2021). Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 9(1), 1-6. https://doi.org/10.24998/maeusabed.869812
AMA Demirtaş A. Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine. Mehmet Akif Ersoy University Journal of Health Sciences Institute. April 2021;9(1):1-6. doi:10.24998/maeusabed.869812
Chicago Demirtaş, Ahu. “Assessing the Susceptibility of Some Gut Bacteria to the Extract from Needles of Turkish Pine”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9, no. 1 (April 2021): 1-6. https://doi.org/10.24998/maeusabed.869812.
EndNote Demirtaş A (April 1, 2021) Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9 1 1–6.
IEEE A. Demirtaş, “Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 9, no. 1, pp. 1–6, 2021, doi: 10.24998/maeusabed.869812.
ISNAD Demirtaş, Ahu. “Assessing the Susceptibility of Some Gut Bacteria to the Extract from Needles of Turkish Pine”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9/1 (April 2021), 1-6. https://doi.org/10.24998/maeusabed.869812.
JAMA Demirtaş A. Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2021;9:1–6.
MLA Demirtaş, Ahu. “Assessing the Susceptibility of Some Gut Bacteria to the Extract from Needles of Turkish Pine”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 9, no. 1, 2021, pp. 1-6, doi:10.24998/maeusabed.869812.
Vancouver Demirtaş A. Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2021;9(1):1-6.