Aflatoxin M1 in UHT cow milk samples collected in Burdur, Turkey

Yıl 2019, Cilt: 7 Sayı: 1, 1 - 7, 30.06.2019

Selinay Başak Erdemli Köse , Fatma Sahindokuyucu Kocasarı , Murat Bayezit

https://doi.org/10.24998/maeusabed.532640

Cited By: 2

Öz

Aflatoxins are secondary metabolites
of toxigenic moulds of the Aspergillus species.
Aflatoxin M₁, a metabolite of the potent carcinogen aflatoxin B₁
(AFB₁) occurs in milks of animals consuming feed contaminated with
AFB₁. The aim of this study was to investigate the occurrence and
levels of aflatoxin M₁ (AFM₁) in UHT milk samples
consumed in Burdur city markets. In 2018, a total of 78 UHT milk samples were
randomly collected from different markets of Burdur. The occurrence and
contamination levels of AFM₁ in the samples were investigated by the
competitive enzyme-linked immunosorbent assay (ELISA) method. Aflatoxin M₁
was detected in 24 of 78 samples (30.77 %) in concentrations between
4.30-127.44 ng/L (mean level: 47.54 ng/L). AFM₁ levels in 11 of
these 24 positive samples were above legal limits of Turkey which is 50 ng/L
for milk samples. It is concluded that the occurrence of AFM₁ in
milk samples in particular may be considered as a possible hazard for public
health.

Anahtar Kelimeler

Aflatoxin M1, UHT milk, occurrence, contamination level

Kaynakça

• Abdallah, M.I.M., Bazalou, M.S., Al-Julaifi, M.Z., 2012. Determination of aflatoxin M1 in concentrations in full-fat cow’s UHT milk sold for consumption in Najran-Saudi regarding its public health significance. Egyptian Journal Applied Science 27, 40-54.
• Akdemir, C., Altintas, A., 2004. Ankara’da işlenen sütlerde aflatoksin-M1 varlığının ve düzeylerinin HPLC ile araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51, 175-179.
• Aydemir-Atasever, M., Adiguzel, G., Atasever, M., Ozlu, H., Ozturan, K., 2010. Occurrence of aflatoxin M1 in UHT milk in Erzurum-Turkey. Kafkas Universitesi Veteriner Fakültesi Dergisi 16, 119-122.
• Bilandzic, N., Bozic, D., Ðokic, M., Sedak, M., Solomun-Kolanovic, B., Varenina, I., Tankovic, S., Cvetni, Z., 2014. Seasonal effect on aflatoxin M1 contamination in raw and UHT milk from Croatia. Food Control 40, 260-264.
• Bilandzic, N., Varenina, I., Solomun, B., 2010. Aflatoxin M1 in raw milk in Croatia. Food Control 21, 1279-1281.
• Creppy, E.E., 2002. Update of survey, regulation and toxic effects of mycotoxins in Europe Toxicology Letters 127, 19-28.
• EC, 2006. Commission Regulation No 1881/ 2006 of 19 December 2006. Setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Communities 364, 5-24.
• Fallah, A.A., 2010. Assessment of aflatoxin M1 contamination in pasteurized and UHT milk marketed in central part of Iran. Food and Chemical Toxicology 48, 988-991.
• FDA, 1996. Sec. 527.400 whole milk low fat, milk skim milk aflatoxin M1 (CPG 7106.210). In FDA compliance policy guides. Washington, DC: US FDA. pp: 219.
• Galvano, F., Galofora, V., Galvona, G., 1996. Occurrence and stability of aflatoxin M1 in milk and milk products: a world wide review. Journal of Food Protection 59(10), 1079-1090.
• Gundinc, U., Filazi, A., 2009. Detection of aflatoxin M1 concentrations in UHT milk consumed in Turkey markets by ELISA. Pakistan Journal of Biological Sciences 12, 653-656.
• Heshmati, A., Milani, J.M., 2010. Contamination of UHT milk by aflatoxin M1 in Iran. Food Control 21, 19-22.
• IARC, 2002. Monograph on the evaluation of carcinogenic risk to humans. World Health Organization. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. Summary of Data Reported and Evaluation, Lyon 82, 171-175.
• Lee, J.E., Kwak, B.M., Ahn, J.H., Jeon, T.H., 2009. Occurrence of aflatoxin M1 in raw milk in South Korea using an immunoaffinity column and liquid chromatography. Food Control 20, 136-138.
• Lin, L.C., Liu, F.M., Fu, Y.M., Shih, D.Y.C., 2004. Survey of aflatoxin M1 contamination of dairy products in Taiwan. Journal of Food and Drug Analysis 12(2), 154-160.
• Marth, E.H., 1979. Aflatoxin in milk, cheese and other dairy products. Marschall Italian  Speciality Cheese Seminars 1-18.
• Martins, M.L., Martins, H.M., 2000. Aflatoxin M1 in raw and ultra high temperature-treated milk commercialized in Portugal. Food Additives and Contaminants 17, 871-874.
• Movassagh, M.H., 2001. Presence of aflatoxin M1 in UHT milk in Tabriz (northwest of Iran). Journal of Food Safety 31, 238-241.
• Oguz, H., Kurtoglu, V., 2000. Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis, British Poultry Science 41, 512-517.
• Oliveira, C.P., Soares, N.F.F., Oliveira, T.V., Baffa Júnior, J.C., Silva, W.A., 2013. Aflatoxin M1 occurrence in ultra high temperature (UHT) treated fluid milk from Minas Gerais/Brazil. Food Control 30(1), 90-92.
• Pittet A., 1998. Natural occurrence of mycotoxins in foods and feeds. A updated review. Revue de Medicine Veterinaire 149(6), 479-492.
• Price, W.D., Lovell, R.A., McChesney, D.G., 1993. Naturally occuring toxins in feedstuffs: Center for Veterinary Medicine Perspective. Journal of Animal Sciences 71, 2556-2562.
• Rahimi, E., Nilchian, Z., Behzadnia, A., 2011. Presence of aflatoxin M1 in pasteurized and UHT milk commercialized in Shiraz, Khuzestan and Yazd, Iran. Journal of Chemical Health Risks 1, 7-10.
• Rahimi, E., Shakerian, A., Jafariyan, M., Ebrahimi, M., Riahi, M., 2009. Occurrence of aflatoxin M1 in raw, pasteurized and UHT milk commercialized in Esfahan and Shahr-e Kord, Iran. Food Security 1, 317-320.
• Roussi, V., Govaris, A., Varagouli, A., Botsoglous, N.A., 2002. Occurrence of aflatoxin M1 in raw and market milk commercialized in Greece. Food Additives and Contaminants 19, 863-868.
• Rustom, I.Y.S., 1997. Aflatoxin in food and feed: occurrence, legislation and inactivation by physical methods. Food Chemistry 59(1), 57-67.
• Sahindokuyucu-Kocasari, F., 2014. Occurrence of aflatoxin M1 in UHT milk and infant formula samples consumed in Burdur, Turkey. Environmental Monitoring and Assessment 186, 6363-6368.
• Sherif, S.O., Salama, E.E., Abdel-Wahhab, M.A., 2009. Mycotoxins and child health:The need for health risk assessment. International Journal of Hygiene and Environmental Health 21(4), 347-368.
• Shundo, L., Navas, S.A., Lamardo, L.C.A., Ruvieri, V., Sabino, M., 2009. Estimate of aflatoxin M1 exposure in milk and occurrence in Brazil. Food Control 20(7), 655-657.
• Shundo, L., Sabino, M., 2006. Aflatoxin M1 in milk by immunoaffinity column cleanup with TLC/HPLC determination. Brazilian Journal of Microbiology 37, 164-167.
• Siddappa, V., Nanjegowda, D.K., Viswanath, P., 2012. Occurrence of aflatoxin M1 in some samples of UHT, raw & pasteurized milk from Indian states of Karnataka and Tamilnadu. Food and Chemical Toxicology 50, 4158-4162.
• Silva, M.V., Janeiro, V., Bando, E., Machinski Jr, M., 2015. Occurrence and estimative of aflatoxin M1 intake in UHT cow milk in Paran a State, Brazil. Food Control 53, 222-225.
• Srivastava, V.P., Bu-Abbas, A., Alaa-Basuny Al-Johar, W., Al-Mufti, S., Siddiqui, M.K.J., 2001. Aflatoxin M1 contamination in commercial samples of milk and dairy products in Kuwait. Food Additives and Contaminants 18, 993-997.
• Tekinsen, K.K., Eken, H.S., 2008. Aflatoxin M1 levels in UHT milk and kaskar cheese consumed in Turkey. Food Chemical and Toxicology 46, 3287-3289.
• TFC, 2011. Gida maddelerindeki belirli bulasanlarin maksimum limitleri hakkında teblig. Resmi Gazete, 29.12.2011, Sayi, 28157. Ankara Basbakanlik Basimevi, Turkey.
• Turkoglu, C., Keyvan, E., 2019. Determination of Aflatoxin M1 and Ochratoxin A in Raw, Pasteurized and UHT Milk in Turkey. Acta Scientiae Veterinariae 47, 1626.
• Unusan, N., 2006. Occurrence of aflatoxin M1 in UHT milk in Turkey. Food and Chemical Toxicology 44, 1897-1900.
• Van Egmond, H.P., 1989. Current situation on regulations of mycotoxins. Overview of tolerances and status of standart methots of sampling and analysis. Food Additives Contaminants 6, 139-188.
• Van Egmond, H.P., Paulch, V.H., 1986. Mycotoxins in milk and milk products. Netherland Milk and Dairy Journal 40, 175-188.
• Var, I., Kabak, B., 2008. Detection of aflatoxin M1 in milk and dairy products consumed in Adana, Turkey. International Journal of Dairy Technology 62, 15-18.
• Whitlow, L.W., Hagler, W.M., Hopkins, B.A., Diaz, D.E., 2000. Mycotoxins in feeds and their effects on dairy cattle. Moormoon’s Feeds Facts 11(3), 1-7.
• Zheng, Z, Sun, P, Wang, J.Q., Zhen, Y.P., Han, R.W., Xu, X.M., 2013. Occurrence of aflatoxin M1 in UHT milk and pasteurized milk in China market. Food Control 29, 198-201.
• Zinedine, A., Gonzalez-Osnaya, L., Soriano, J.M., Molto, J.C., Idrissi, L., Manes, J., 2007. Presence of aflatoxin M1 in pasteurized milk from Morocco. International Journal of Food Microbiology 114, 25-29.