değerlendirme süreci Mantar Dergisi 2147-6845 Selçuk Üniversitesi Mycology Mikoloji Çoklu İlaca Dirençli Bakteri İzolatlarına Karşı Laetiporus sulphureus Ekstraktlarının Antibakteriyel Aktivitesi Antibacterial Activity of Laetiporus sulphureus Extracts Against Multidrug Resistant Bacterial Isolates https://orcid.org/0000-0002-3663-7412 Akkoyunlu Ayşegül DUZCE UNIVERSITY https://orcid.org/0000-0002-1506-1549 Dulger Gorkem DÜZCE ÜNİVERSİTESİ, TIP FAKÜLTESİ https://orcid.org/0000-0002-3184-2652 Dülger Başaran DÜZCE ÜNİVERSİTESİ 04 30 2025 16 1 1 7 10 30 2024 11 19 2024 Copyright © 2010, Mantar Dergisi 2010 Mantar Dergisi

Laetiporus sulphureus (Bull.) Murrill (Polyporaceae) etanol ekstraktlarının, çoklu ilaca dirençli bakteri izolatlarına karşı antibakteriyel aktivitesi agar kuyucuk difüzyon ve dilüsyon yöntemleri kullanılarak test edilmiştir. Bulgulara göre, L. sulphureus'un antibakteriyel aktivitesi ekstrakt konsantrasyonuna bağlı olarak değişiklik göstermektedir. Diğer dozlarda aktivite gözlenmezken, etanolik ekstrakt 200 mg/mL'de 10,2 ile 17,4 mm arasında, 100 mg/mL'de 8,2 ile 12,6 mm arasında ve 50 mg/mL'de 9,2 ile 9,8 mm arasında inhibisyon zonları göstermiştir. Ekstrakta en az duyarlı bakteri 200 mg/mL'de 10,2 mm ile Klebsiella pneumoniae iken, en duyarlı bakteri 200 mg/mL'de 17,4 mm ile Bacillus subtilis olmuştur. Ekstraktın Staphylococcus aureus, B. subtilis ve K. pneumoniae'ye karşı minimum inhibitör konsantrasyonu (MİK) 25 mg/mL ile 200 mg/mL arasında değişmektedir. B. subtilis, S. aureus ve Pseudomonas aeruginosa için ekstraktın minimum bakterisidal konsantrasyonu (MBK) sırasıyla 50 mg/mL ve 200 mg/mL olarak kaydedilmiştir; ancak K. pneumoniae için herhangi bir MBK bulunamamıştır. S. aureus en yüksek direnç seviyesini (%70) gösterirken, P. aeruginosa en düşük direnç seviyesini (%10) göstermiştir. Pefloksasin, siprofloksasin, septrin ve sparfloksasin en güçlü antibiyotikler olarak tanımlanmıştır. Bulgular, L. sulphureus'un bir antibakteriyel ajan olarak potansiyel taşıdığını, ancak etkinliğinin ekstrakt konsantrasyonuna ve bakteri türüne göre değiştiğini göstermektedir.

Ethanol extracts from Laetiporus sulphureus (Bull.) Murrill (Polyporaceae) were tested for their antibacterial activity against multidrug-resistant bacterial isolates using agar well diffusion and dilution procedures. According to the findings, L. sulphureus's antibacterial activity differed depending on the extract concentration. While no activity was observed at other dosages, the ethanolic extract demonstrated inhibitory zones ranging from 10.2 to 17.4 mm at 200 mg/mL; 8.2 to 12.6 mm at 100 mg/mL; and 9.2 to 9.8 mm at 50 mg/mL. Klebsiella pneumoniae (10.2 mm at 200 mg/mL) was the least susceptible to the extract, while Bacillus subtilis (17.4 mm at 200 mg/mL) was the most sensitive. The extract’s minimum inhibitory concentration (MIC) against Staphylococcus aureus, B. subtilis, and K. pneumoniae ranged between 25 mg/mL and 200 mg/mL. For B. subtilis, S. aureus, and Pseudomonas aeruginosa, the minimum bactericidal concentration (MBC) of the extract was recorded at 50 mg/mL and 200 mg/mL, respectively; however, no MBC was found for K. pneumoniae. S. aureus exhibited the highest level of resistance (70%), while P. aeruginosa demonstrated the lowest level of resistance (10%). Pefloxacin, ciprofloxacin, septrin, and sparfloxacin were identified as the most potent antibiotics. The findings suggest that L. sulphureus has potential as an antibacterial agent, though its effectiveness varies according to extract concentration and bacterial species.

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