Otolitlerde Alizarin Kırmızı S İşaretleme: Diplodus vulgaris Yavrularının Büyüme Dinamikleri Üzerine Bir Çalışma
Abstract
Bu çalışma, Çanakkale Boğazı'ndaki genç Diplodus vulgaris balıklarının yaş ve büyüme hızlarını araştırmaktadır. Günlük büyüme artışlarını analiz etmek için otolitleri incelemek için Alizarin Red S (ARS) işaretlemesini kullanan araştırma, genç balıkların yaşını belirlemede güvenilir bir yöntem olarak otolitlerdeki günlük büyüme halkalarının doğruluğunu kanıtlamayı amaçlamıştır. 65 örnek, gençlerin hayatta kalma oranlarını olumsuz etkilemeyecek optimal işaretleme koşullarını belirlemek için değişen konsantrasyonlarda ARS'ye maruz bırakılmıştır. İşlemden sonra balıklar kontrol edilen ortamlara salınmış ve otolitlerdeki ARS işaretlerinin birleşme ve görünürlüğünü değerlendirmek için bir süre izlenmiştir. Sonuçlar, balık boyutu ile yaş arasında net bir korelasyon olduğunu göstermiş ve otolitlerde günlük artış oluşumunu destekleyen tutarlı bir günlük büyüme hızını ortaya koymuştur. ARS işlemi ile ilişkili ölüm oranı kabul edilebilir sınırlar içindeydi ve bu yöntemin ölümcül olmayan yaş ve büyüme çalışmaları için uygunluğunu doğrulamıştır. Bu araştırma, genç balık büyüme modellerini incelemek için ARS işaretlemenin etkinliğini vurgulamanın yanı sıra, Çanakkale Boğazı'ndaki D. vulgaris'in yaşam tarihini daha iyi anlamaya katkıda bulunarak, balıkçılık yönetimi ve koruma çabaları için değerli içgörüler sunmaktadır.
Ethical Statement
Deney hayvanlarının bakımı ve kullanımı, [Çanakkale Onsekiz Mart Üniversitesi, Hayvan Deneyleri Yerel Etik Komitesi] tarafından onaylanan hayvan refahı yasaları, yönergeleri ve politikalarına uygun olarak gerçekleştirilmiştir [2018/02-10].
Supporting Institution
TÜBİTAK
Project Number
118O321
References
- Agresti, A. (2002). Categorical data analysis. John Wiley & Sons. https://doi.org/10.1002/0471249688
- Amos, K. H., Applebaum, S., & McGowan, M. F. (1998). Safe concentrations of alizarin complexone for marking otoliths of larval and juvenile fish. Transactions of the American Fisheries Society, 127(4), 600-605.
- Campana, S. E., & Thorrold, S. R. (2001). Otoliths, increments, and elements: keys to a comprehensive understanding of fish populations? Canadian Journal of Fisheries and Aquatic Sciences, 58(1), 30-38. https://doi.org/10.1139/f00-177
- Campana, S. E., Neilson, J. D. (1985). Microstructure of fish otoliths. Canadian Journal of Fisheries Aquatic Sciences, 42(5), 1014-1032. https://doi.org/10.1139/f85-127
- Fowler, A. J. (1990). Validation of annual growth increments in the otoliths of a small, tropical coral reef fish. Marine Ecology Progress Series, 64, 25-38.
- Fowler, A. J. (1995). Age determination and growth. In J. R. Secor, J. M. Dean, & S. E. Campana (Eds.), Recent developments in fish otolith research (pp. 555-568). University of South Carolina Press.
- Green, B. S., & McCormick, M. I. (2001). Effects of larval growth history and egg size on metamorphosis in a coral reef fish. Oecologia, 128(3), 356-362.
- Hammer, Ø., Harper, D.A. T., & Ryan, P. D. (2001), PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 4(1), 4.
- Jones, C. M. (1992). Development and application of the otolith increment technique. In D. K. Stevenson & S. E. Campana (Eds.), Otolith microstructure examination and analysis (pp. 1-11). Canadian Special Publication of Fisheries and Aquatic Sciences.
- Morales-Nin, B. (2000). Review of the growth regulation processes of otolith daily increment formation. Fisheries Research, 46(1-3), 53-67. https://doi.org/10.1016/S0165-7836(00)00133-8
- Morales-Nin, B., & Aldebert, Y. (1997). Growth of juvenile Diplodus sargus and Diplodus vulgaris in the Gulf of Lions (NW Mediterranean). Scientia Marina, 61(Suppl. 1), 65-77.
- Peck, M. A., Huebert, K. B., & Llopiz, J. K. (2012). Chapter 3 - Intrinsic and extrinsic factors driving match–mismatch dynamics during the early life history of marine fishes. In Woodward, G., Jacob, U., & O’Gorman, E. J. (Eds.), Advances in ecological research Volume 47 (pp. 177-302). https://doi.org/10.1016/B978-0-12-398315-2.00003-X
- Secor, D. H., Dean, J. M., & Laban, E. H. (1991). Manual for otolith removal and preparation for microstructural examination. Electric Power Research Institute and Belle W. Baruch Institute for Marine Biology and Coastal Research.
- Tsukamoto, K., Arai, T., & Kotake, A. (1989). A review of the research on the early life history of eels and its application to studies of the population recruitment mechanism. Bulletin of the Ocean Research Institute, University of Tokyo, 26, 1-66.
- Wilson, D. T., & Mccormick, M. I. (1999). Microstructure of settlement-marks in the otoliths of tropical reef fishes. Marine Biology, 134(1), 29-41. https://doi.org/10.1007/s002270050522
Abstract
This study investigates the age and growth rates of juvenile Diplodus vulgaris in the Çanakkale Strait, utilizing Alizarin Red S (ARS) marking to analyze otoliths for daily growth increments. The research aimed to validate the daily deposition of growth rings in otoliths as a reliable method for age determination in young fish. A total of 65 specimens were subjected to ARS immersion at varying concentrations to identify the optimal marking conditions that would not adversely affect the survival rates of the juveniles. Post-treatment, the fish were released into controlled environments and monitored over a period to assess the incorporation and visibility of ARS marks in the otoliths. The results demonstrated a clear correlation between fish size and age, with a consistent daily growth rate that supports the hypothesis of daily increment formation in otoliths. The mortality rate associated with ARS treatment was within acceptable limits, confirming the method's viability for non-lethal age and growth studies. This research not only underscores the effectiveness of ARS marking in studying juvenile fish growth patterns but also contributes to a better understanding of the life history of D. vulgaris in the Çanakkale Strait, offering valuable insights for fisheries management and conservation efforts.
Ethical Statement
The care and use of experimental animals complied with [Çanakkale Onsekiz Mart University, Animal Experiments Local Ethics Committee] animal welfare laws, guidelines and policies as approved by [2018/02-10].
Supporting Institution
TÜBİTAK
Project Number
118O321
References
- Agresti, A. (2002). Categorical data analysis. John Wiley & Sons. https://doi.org/10.1002/0471249688
- Amos, K. H., Applebaum, S., & McGowan, M. F. (1998). Safe concentrations of alizarin complexone for marking otoliths of larval and juvenile fish. Transactions of the American Fisheries Society, 127(4), 600-605.
- Campana, S. E., & Thorrold, S. R. (2001). Otoliths, increments, and elements: keys to a comprehensive understanding of fish populations? Canadian Journal of Fisheries and Aquatic Sciences, 58(1), 30-38. https://doi.org/10.1139/f00-177
- Campana, S. E., Neilson, J. D. (1985). Microstructure of fish otoliths. Canadian Journal of Fisheries Aquatic Sciences, 42(5), 1014-1032. https://doi.org/10.1139/f85-127
- Fowler, A. J. (1990). Validation of annual growth increments in the otoliths of a small, tropical coral reef fish. Marine Ecology Progress Series, 64, 25-38.
- Fowler, A. J. (1995). Age determination and growth. In J. R. Secor, J. M. Dean, & S. E. Campana (Eds.), Recent developments in fish otolith research (pp. 555-568). University of South Carolina Press.
- Green, B. S., & McCormick, M. I. (2001). Effects of larval growth history and egg size on metamorphosis in a coral reef fish. Oecologia, 128(3), 356-362.
- Hammer, Ø., Harper, D.A. T., & Ryan, P. D. (2001), PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 4(1), 4.
- Jones, C. M. (1992). Development and application of the otolith increment technique. In D. K. Stevenson & S. E. Campana (Eds.), Otolith microstructure examination and analysis (pp. 1-11). Canadian Special Publication of Fisheries and Aquatic Sciences.
- Morales-Nin, B. (2000). Review of the growth regulation processes of otolith daily increment formation. Fisheries Research, 46(1-3), 53-67. https://doi.org/10.1016/S0165-7836(00)00133-8
- Morales-Nin, B., & Aldebert, Y. (1997). Growth of juvenile Diplodus sargus and Diplodus vulgaris in the Gulf of Lions (NW Mediterranean). Scientia Marina, 61(Suppl. 1), 65-77.
- Peck, M. A., Huebert, K. B., & Llopiz, J. K. (2012). Chapter 3 - Intrinsic and extrinsic factors driving match–mismatch dynamics during the early life history of marine fishes. In Woodward, G., Jacob, U., & O’Gorman, E. J. (Eds.), Advances in ecological research Volume 47 (pp. 177-302). https://doi.org/10.1016/B978-0-12-398315-2.00003-X
- Secor, D. H., Dean, J. M., & Laban, E. H. (1991). Manual for otolith removal and preparation for microstructural examination. Electric Power Research Institute and Belle W. Baruch Institute for Marine Biology and Coastal Research.
- Tsukamoto, K., Arai, T., & Kotake, A. (1989). A review of the research on the early life history of eels and its application to studies of the population recruitment mechanism. Bulletin of the Ocean Research Institute, University of Tokyo, 26, 1-66.
- Wilson, D. T., & Mccormick, M. I. (1999). Microstructure of settlement-marks in the otoliths of tropical reef fishes. Marine Biology, 134(1), 29-41. https://doi.org/10.1007/s002270050522
Details
| Primary Language | English |
|---|---|
| Subjects | Fish Biology, Aquaculture and Fisheries Stock Assessment |
| Journal Section | Research Articles |
| Authors | |
| Project Number | 118O321 |
| Publication Date | June 30, 2024 |
| Submission Date | March 7, 2024 |
| Acceptance Date | March 28, 2024 |
| Published in Issue | Year 2024 Volume: 6 Issue: 1 |
