Iran Tebriz Ostad Alinasab Hastanesi’nin örneklerinden elde edilen Escherichia coli izolatlarında genişletilmiş-spektrum beta-laktamoz (TEM1) geninin araştırılması

Yıl 2016, , 35 - 40, 04.03.2016

Azizeh Dizaji Azizeh Shadi Dizajı Reza Fathi Abolfazl Sales Abolfazl Jafari Sales

https://doi.org/10.5472/MMJoa.2901.06

Cited By: 6

Öz

Amaç: Antibiyotik direncinin gelişmesi bakteriyel enfeksiyonların
kontrolünde önemli bir sorundur. Bu çalışma, antibiyotik
duyarlılık profillerini değerlendirmek ve Tebriz’de Ostad Alinasab
Hastane’sinin klinik örnekleriden elde edilen E.coli izolatlarında
blaTEM geni varlığını değerlendirmek amacıyla planlanmıştır.
Gereç ve Yöntemler: E. coli (100 izolatları) geleneksel bakteriyolojik
testler ile elde edilmiş ve daha sonra antibiyotic duyarlılık
testleri Kirby-Bauer yöntemine göre değerlendirilmiştir.
Doğrulayıcı testler ise “Combinde disk” testleri ile gerçekleştirilmiştir.
Son olarak blaTEM geni varlığı polymeraz zincir reaksiyon
(PCR) tekniği kullanılarak değerlendirilmiştir.
Bulgular: Yüz E. coli izolatından %23’nün blaTEM genini
içerdiği belirlenmiştir. İzolatlarının %18’inde Seftazidim, %
30’unda sefotaksim direnci saptanmıştır. Izolatların % 46’sında
EBSL sentezi belirlenmiş ve bu izolatların % 23’de de blaTEM
geninin varlığı saptanmıştır.
Sonuç: E. coli izolatlarında saptanan üçüncü kuşak sefalosporinlere
karşı yüksek direnç uygunsuz antibiyotik kullanımından
kaçınılması gerektiğinin ve tedavi öncesi doğru duyarlılık testlerinin
uygulanması gerektiğinin önemin göstermektedir.

Anahtar Kelimeler

Geniş spektrum beta-laktamaz (GSBL), blaTEM geni, Escherichia coli, Polimeraz zincir reaksiyonu

Kaynakça

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