Prenatal sıçan mesanesi üzerine elektromanyetik dalganın etkilerinin morfolojik ve biyokimyasal olarak incelenmesi

Abstract

Amaçlar: Bu çalışmanın amacı, dijital mobil telefonlarından gelen

ve en yüksek spesifik absorbsiyon oranı (Specific Absorbtion

Rate-SAR) değeri 1,79W/kg olan dijital selüler sistem (DSS)

1800-MHz radyasyonunun erkek sıçan mesanesi üzerine etkilerini

araştırmaktır.

Gereç ve Yöntem: Sıçanlara mobil telefondan stand-by

(Stand-by Fetal grup) ya da kesintisiz iletim modunda yayılan

elektromagnetik dalga (EMD Fetal grup), günde 2 saat, embriyonik

14. günden doğuma kadar ya da doğumdan sonraki 60. güne kadar

(Stand-by ve EMD grupları) uygulanmıştır. Deney gruplarındaki

tüm hayvanların mesaneleri postnatal 60. günde eter anestezisi

altında alınmış ve ışık ve elektron mikroskopik değerlendirmeler

için hazırlanmıştır. Ürotelyumun bariyer fonkisyonu zonula

okludens 1 ve E-kaderin immunhistokimyası ve transmisyon

elektron mikroskobik inceleme için rutenyum kırmızısı boyaması

ile değerlendirilmiştir. Oksidatif hasar biyokimyasal teknikler ile

değerlendirilmiştir.

Bulgular: Elektromagnetik dalga grubunda, ürotelyum

dökülmeleri, mast hücre sayısında artış, sıkı bağlantılarda hasar

ve interselüler alanda genişlemeler gözlenmiştir. Son olarak,

kontrol grubu ile kıyaslandığında, tüm gruplarda malondialdehit

seviyesinde yükselme ve glutatyon seviyesinde düşüş gözlenmiştir.

Sonuç: Elektromagnetik dalga, maruz kalınan yoğunluk ve

süreye paralel olarak, gelişim sürecindeki mesane üzerine olumsuz

etki yapmaktadır. Bu değişiklikler mesanede enflamatuvar

hastalıklara sebep olabilir.

Keywords

• Cep telefonu,Elektromanyetik dalga,Mesane,ZO-1,E-kaderin

The morphological and biochemical investigation of prenatal electromagnetic wave effects on urinary bladder in rats

Abstract

Objectives: The aim of the study was to investigate the effects of

digital cellular system (DCS) 1800-MHz radiation of a common

digital mobile phone which has the highest specific absorption rate

value of 1.79 W/kg on the urinary bladders of male rats.

Materials and Methods: Rats exposed to electromagnetic

wave (EMW) emmited by mobile phones on stand-by (Stand-by

Fetal Group) or discontinious transmission mode (EMW Fetal

group), 2 hours per day, from embryonic day 14 until parturition

or until postnatal day 60 (Stand-by Fetal Group). The urinary

bladders of the animals in experimental groups were removed at

postnatal day 60 and prepared for light and electron microscopical

investigation. The barrier function of urothelium was evaluated

using zonula occludens 1 and E-cadherin immunohistochemistry

and ruthenium red staining for transmission electron microscopy.

Oxidative damage were evaluated by biochemical techniques.

Results: EMW wave group showed desquamation of urothelial

cells, increased number of mast cells, degeneration of tight junctions and

dilatation of intercellular space. Finally, increase in the malondialdehyde

and decrease in the glutathione levels were observed in all experimental

groups when compared to Those in the control group.

Conclusion: Exposure intensity and time length correlate with

adverse effects in developing period of the urinary bladder. These

changes may lead to urinary bladder inflammatory disorders.

Keywords

• Cellular phone,Electromagnetic wave,Urinary bladder,ZO-1,E-cadherin

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