Keton cisimlerinin insan meme kanseri hücrelerinde (MCF-7) canlılık üzerine etkileri

Abstract

Amaç: Kanser hücreleri aerobik koşullar altında Warburg etkisi

olarak bilinen artmış glikolitik fenotipini sergilerler. Ayrıca,

son zamanlarda yapılan çalışmalar, kanser hücrelerinin glikoza

bağımlı olduğunu ve keton cisimlerini birincil bir enerji kaynağı

olarak kullanamadığını göstermiştir. Bu çalışmada, insan meme

kanseri hücrelerinin birincil enerji kaynağı olarak keton cisimlerini

kullanamayacağını varsaydık ve keton cisimlerinin hücre canlılığı

üzerine etkisini araştırdık.

Gereç ve Yöntem: Bu çalışmada insan meme kanseri hücre hattı

(MCF-7) ve insan foreskin fibroblast hücre hattı (HFF) kullanıldı.

Her iki hücre hattında glukoz içeren ve içermeyen ortamlarda

keton cisimleri olan asetoasetat ve beta-hidroksibütiratın hücre

canlılığına etkisini inceledik.

Bulgular: Keton cisimlerini içeren ortamda MCF-7

hücrelerinde hücre canlılığı kontrol ortamı ile karşılaştırıldığında

azalırken, kontrol olarak kullanılan HFF hücrelerinde herhangi bir

değişiklik görülmedi.

Sonuç: Elde edilen veriler ışığında, keton cisimlerinin

kullanılması ile gerçekleştirilecek beslenmenin meme kanseri

tedavisinde yeni bir strateji olabileceğini düşünmekteyiz.

Keywords

• Asetoasetat,Beta-hidroksibütirat,Keton cisimleri,MCF-7 kanser hücreleri

Effect of ketone bodies on viability of human breast cancer cells (MCF-7)

Abstract

Objective: Cancer cells exhibit an elevated glycolytic phenotype

under aerobic conditions, which is known as the Warburg effect.

Recent studies have also shown that cancer cells are glucosedependent

and cannot use ketone bodies as a primary source of

energy. In this study, we have investigated the effects of ketone

bodies on viability of breast cancer cells considering that breast

cancer cells would not use ketone bodies as a primary energy

source.

Materials and Methods: In this study we have used MCF-7

cells, which are breast cancer cells that cannot use ketone bodies

as a primary energy source and human foreskin fibroblast cells

(HFF) as controls. We measured cell viability in both cells cultured

in the presence or absence of glucose as well as the ketone bodies

acetoacetate and beta-hydroxybutyrate.

Results: Cell viability was significantly decreased in response

to ketone bodies compared with control media in MCF-7 cells

whereas in control cells (HFF) cell viability was not changed.

Conclusion: In light of the data obtained, we suggest that

dietary manipulation with the use of ketone bodies may be a new

therapeutic strategy for breast cancer.

Keywords

• Acetoacetate,Beta-hydroxybutyrate,ketone bodies,MCF-7 cancer cells

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