BibTex RIS Kaynak Göster

MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN

Yıl 1999, Cilt: 12 Sayı: 1, 25 - 28, 03.12.2016

Öz

Objective: Activation of cholinergic muscarinic receptors leads to activation of membrane - bound phospholipase C, leading to generation of IP3 and diacylglycérol, The present study aimed at measuring muscarinic receptor-mediated phosphoinositide breakdown in three different regions of rat brain.
Methods: Cortical, hippocampal and striatal slices labeled with [3H] myo-inositol were incubated with muscarinic agonist carbachol (Cch) and LiCI. Reaction was terminated by the addition of chloroform/methanol and labeled inositol phosphates were extracted from tissue. Individual [3H]- inositol phosphates were separated using anion exchange chromatography.
Results: Cch caused a rise in IP, formation, where the increase over basal in the cortex, hippocampus and striatum were 98%, 73% and 45%, respectively.
Conclusion: Phosphoinositide turnover was
stimulated by carbachol in all brain structures studied, with the most pronounced effects observed in the cortex and hippocampus.
Key Words: Muscarinic receptors, inositol
phosphates, carbachol, rat brain

Kaynakça

  • Waelbroeck M, Tastenoy M, Camus J, Christophe J. binding of selective antagonists to four muscarinic receptors (Mt to M4) in rat forebrain. Mol. Pharmacol. 1990;38:267-273.
  • Bonner Tl, Buckley ilJ, Young AC and Brann MR. Identification of a family of muscarinic acetylcholine receptor genes. Science 1987;237:527-532.
  • Bonner Tl, Young AC, Brann MR and Buckley IYJ. Cloning and expression of the human and rat M5 muscarinic acetylcholine receptor genes, neuron 1988;1:403-410.
  • Peralta EG, Ashkenazi A, Winslow JW, Smith DU, Ramachandran J and Capon DJ. Distinct primary structures, ligand binding properties and tissue- spicific expression of four human muscarinic acetylcholine receptors. EMBO J. 1987:6:3923- 3929.
  • Buckley MJ, Bonner Tl and Brann MR. Localization of a familiy of muscarinic receptor mRMAs in rat brain. J. Meurosci. 1988:8:4646-4652.
  • Yasuda RP, Ciesla W, Flores LR, Wall SJ, Li M, Satkus 5/1, Weisstein JS, Spagnola BV, Wolfe BB. Development of antisera specific for M4 and M5 muscarinic cholinergic receptors: Distribution of M4 and M5 receptors in rat brain. Mol. Pharmacol. 1992;43:149-157.
  • Brann MR, Buckley nj, Bonner Tl. The striatum and cerebral cortex express different muscarinic receptor mRMAs. FEBS Lett. 1988;230:90-94.
  • Mei L, Roeske WR, Yamamura til. Molecular pharmacology of muscarinic receptors heterogeneity. Life Sci. 1989;45:1831-1851.
  • Dorje F, Levey Al and Brann MR. Immunological detection of muscarinic receptor subtype proteins (MrM5) in rabbit peripheral tissues. Mol. Pharmacol. 1991;40:459-462.
  • tlersch SM, Gutekunst CA, Rees HD, Heilman CJ and Levey Al. Distrubition of M ¡-M4 muscarinic
  • Marmara Medical Journal Volume 12 No: 1 January 1999
  • receptor proteins in the rat striatum: Light and electron microscopic immunocytochemistry using subtype specific antisera. J. rieurosci. 1994;14:3351-3363.
  • Peralta EG, Ashkenazi A, Winslow JW, Ramachandran J and Capon DJ, Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypes. nature 1988;334:434-437.
  • Bonner Tl. new subtypes of muscarinic acetylcholine receptors. Trends. Pharmacol. Sci. 1989;(Suppl.):l 1-15.
  • Parker EM, Kameyama K, Fligashijima T and Ross EM. Reconstitutively active G-protein-coupled receptors puriPied from baculovirus-infected insect cells. J. Biol. Chem. 1991;266:519-527.
  • Gonzales RA, Crews FT. Characterization of the cholinergic stimulation of phosphoinositide hydrolysis in rat brain slices. J. Meurosci. 1984;4:3120-3127.
  • Brown ED, Kendall DA, Mahorski SR. Inositol phospholipid hydrolysis in rat cerebral cortical
  • slices. I. Receptor characterization. J. neurochem. 1984;42:1379-1387.
  • Fisher KS, Fiqueiredo JC, Bartus RT. Differential stimulation of inositol phospholipid turnover in brain by analogs of oxotremorine. J. neurochem. 1984;43:l 171-1 179.
  • Fisher KS, Bartus RT. Regional differences in the coupling of muscarinic receptors to inositol phospholipid hydrolysis in guinea pig brain. J. neurochem 1985;45:1085-1095.
  • Berridge MJ. Rapid accumulation of inositol triphosphate reveals that agonists hydrolyse phosphoinositides instead of phosphatidylinositol. Biochem. J. 1983;212:849-858.
  • Lowry Oft, Rosebrough nJ, Farr AL and Randall RJ. Protein measurement with the folin phenol reagent.
  • J. Biol. Chem. 1951;193:265-275.
  • Tonnaer JADM, Cheung CL, DeBoer T. cGMP Formation and phosphoinositide turnover in rat brain slices are mediated by pharmacologically distinct muscarinic acetylcholine re
Yıl 1999, Cilt: 12 Sayı: 1, 25 - 28, 03.12.2016

Öz

Kaynakça

  • Waelbroeck M, Tastenoy M, Camus J, Christophe J. binding of selective antagonists to four muscarinic receptors (Mt to M4) in rat forebrain. Mol. Pharmacol. 1990;38:267-273.
  • Bonner Tl, Buckley ilJ, Young AC and Brann MR. Identification of a family of muscarinic acetylcholine receptor genes. Science 1987;237:527-532.
  • Bonner Tl, Young AC, Brann MR and Buckley IYJ. Cloning and expression of the human and rat M5 muscarinic acetylcholine receptor genes, neuron 1988;1:403-410.
  • Peralta EG, Ashkenazi A, Winslow JW, Smith DU, Ramachandran J and Capon DJ. Distinct primary structures, ligand binding properties and tissue- spicific expression of four human muscarinic acetylcholine receptors. EMBO J. 1987:6:3923- 3929.
  • Buckley MJ, Bonner Tl and Brann MR. Localization of a familiy of muscarinic receptor mRMAs in rat brain. J. Meurosci. 1988:8:4646-4652.
  • Yasuda RP, Ciesla W, Flores LR, Wall SJ, Li M, Satkus 5/1, Weisstein JS, Spagnola BV, Wolfe BB. Development of antisera specific for M4 and M5 muscarinic cholinergic receptors: Distribution of M4 and M5 receptors in rat brain. Mol. Pharmacol. 1992;43:149-157.
  • Brann MR, Buckley nj, Bonner Tl. The striatum and cerebral cortex express different muscarinic receptor mRMAs. FEBS Lett. 1988;230:90-94.
  • Mei L, Roeske WR, Yamamura til. Molecular pharmacology of muscarinic receptors heterogeneity. Life Sci. 1989;45:1831-1851.
  • Dorje F, Levey Al and Brann MR. Immunological detection of muscarinic receptor subtype proteins (MrM5) in rabbit peripheral tissues. Mol. Pharmacol. 1991;40:459-462.
  • tlersch SM, Gutekunst CA, Rees HD, Heilman CJ and Levey Al. Distrubition of M ¡-M4 muscarinic
  • Marmara Medical Journal Volume 12 No: 1 January 1999
  • receptor proteins in the rat striatum: Light and electron microscopic immunocytochemistry using subtype specific antisera. J. rieurosci. 1994;14:3351-3363.
  • Peralta EG, Ashkenazi A, Winslow JW, Ramachandran J and Capon DJ, Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypes. nature 1988;334:434-437.
  • Bonner Tl. new subtypes of muscarinic acetylcholine receptors. Trends. Pharmacol. Sci. 1989;(Suppl.):l 1-15.
  • Parker EM, Kameyama K, Fligashijima T and Ross EM. Reconstitutively active G-protein-coupled receptors puriPied from baculovirus-infected insect cells. J. Biol. Chem. 1991;266:519-527.
  • Gonzales RA, Crews FT. Characterization of the cholinergic stimulation of phosphoinositide hydrolysis in rat brain slices. J. Meurosci. 1984;4:3120-3127.
  • Brown ED, Kendall DA, Mahorski SR. Inositol phospholipid hydrolysis in rat cerebral cortical
  • slices. I. Receptor characterization. J. neurochem. 1984;42:1379-1387.
  • Fisher KS, Fiqueiredo JC, Bartus RT. Differential stimulation of inositol phospholipid turnover in brain by analogs of oxotremorine. J. neurochem. 1984;43:l 171-1 179.
  • Fisher KS, Bartus RT. Regional differences in the coupling of muscarinic receptors to inositol phospholipid hydrolysis in guinea pig brain. J. neurochem 1985;45:1085-1095.
  • Berridge MJ. Rapid accumulation of inositol triphosphate reveals that agonists hydrolyse phosphoinositides instead of phosphatidylinositol. Biochem. J. 1983;212:849-858.
  • Lowry Oft, Rosebrough nJ, Farr AL and Randall RJ. Protein measurement with the folin phenol reagent.
  • J. Biol. Chem. 1951;193:265-275.
  • Tonnaer JADM, Cheung CL, DeBoer T. cGMP Formation and phosphoinositide turnover in rat brain slices are mediated by pharmacologically distinct muscarinic acetylcholine re
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Bölüm Original Research
Yazarlar

Hülya Cabadak Bu kişi benim

Beki Kan Bu kişi benim

Yayımlanma Tarihi 3 Aralık 2016
Yayımlandığı Sayı Yıl 1999 Cilt: 12 Sayı: 1

Kaynak Göster

APA Cabadak, H., & Kan, B. (2016). MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN. Marmara Medical Journal, 12(1), 25-28.
AMA Cabadak H, Kan B. MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN. Marmara Med J. Haziran 2016;12(1):25-28.
Chicago Cabadak, Hülya, ve Beki Kan. “MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN”. Marmara Medical Journal 12, sy. 1 (Haziran 2016): 25-28.
EndNote Cabadak H, Kan B (01 Haziran 2016) MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN. Marmara Medical Journal 12 1 25–28.
IEEE H. Cabadak ve B. Kan, “MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN”, Marmara Med J, c. 12, sy. 1, ss. 25–28, 2016.
ISNAD Cabadak, Hülya - Kan, Beki. “MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN”. Marmara Medical Journal 12/1 (Haziran 2016), 25-28.
JAMA Cabadak H, Kan B. MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN. Marmara Med J. 2016;12:25–28.
MLA Cabadak, Hülya ve Beki Kan. “MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN”. Marmara Medical Journal, c. 12, sy. 1, 2016, ss. 25-28.
Vancouver Cabadak H, Kan B. MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN. Marmara Med J. 2016;12(1):25-8.