GENOTYPE ANALYSIS OF HEPATITIS B VIRUS IN THE TURKISH POPULATION USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM PATTERNS

Yıl 2001, Cilt: 14 Sayı: 4, 213 - 216, 03.12.2016

Pınar Uysal Naz Oğuzoğlu Ayşe Özer

Öz

Objective: Six different HBV genotypes have been defined comparing the complete HBV genome. The distribution of genotypes among infected populations varies geographically. A relationship between HBV genotypes and the development of chronic liver disease has been proposed. Therefore, this study was designed to classify HBV genotypes in the Turkish population using restriction fragment length polymorphism (RFLP) analysis of the S gene region.
Methods: The HBV DNA extracted from forty- eight serum samples were subjected to polymerase chain reaction (PCR) using primers from the S region of HBV genome. Restriction enzymes, Hph I (Genotype F), Nci I (Genotype E), Alw I (Genotype C), Ear I (Genotype B), and Nia IV (Genotype A and D), were used for RFLP analysis of amplified product of HBV DNA.
Results: In the 48 serum samples investigated, 3 different genotypes were detected. These were as follows; Genotype D: 41 (85.41%), Genotype A: 5(10.41%), and Genotype F: 2 (4.16%).
Conclusion: This study shows a molecular heterogeneity of HBV in the Turkish population
with at least 3 different genotypes observed in the 48 samples studied. The genotype D was by far the most common genotype in the Turkish population as is the case in all other Mediterranean countries.
Key Words: Hepatitis B virus, Genotypes, S gene, Restriction fragment length polymorphism.

Kaynakça

• /. Ozer A. The molecular structure of the hepatitis B virus (Review). Marmara Med J 1995;8:157-162.
• Courouce AM, Holland P, Muller PV, Soulier JP. HBs antigen subtypes: Proceedings of the International Workshop on HBs antigen subtypes. Bibl Haematol 1976;42:1-158.
• Courouce-Pauty AM, Lemaire M, Roux JP. Hew hepatitis B surface antigen subtypes inside the ad category. Vox Sang 1978;35:304-308.
• Horder H, Courouce AM, Magnius L. Complete genomes. Phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 1994; 198:489-503.
• Okamoto H, Tsuda F, Sakugawa H, et al. Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol 1988:69:2575- 2583.
• Telenta PFS, Poggio GP, Lopez JL, Gonzalez J, Lemberg A, Campos RH. Increased prevalence of genotype F hepatitis B virus isolates in Buenos Aires, Argentina. J Clin Microbiol 1997;35:1873-1875.
• Mizokami M, Hakano T, Orito E, et al. Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns. FEBS Letters 1999;450:66-71.
• Lindh M, Horai P, Dhillon AP, Furuta Y, Horkrans G. Hepatitis B virus carriers without precore mutations in HbeAg-negative stage show more severe liver damage. Hepatology 1996;24:494-501.
• Shiina S, Fujino H, Uta Y, et al. Relationship of HbsAg subtypes with Hbe/Ag/anti-Hbe status and chronic liver disease. Am J Gastroenterol 1991;86:866-871.
• Lindh M, Andersson AS, Gusdai A. Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus-large-scale analysis using a new genotyping method. J Infect Dis 1997;175:1285-1293.
• Zhang X, Zoulim F, Habersetzer F, Xiong 5, Trepo C. Analysis of hepatitis B virus genotypes and pre-core region variability during interferon treatment of HBe antigen negative chronic hepatitis. Br J Med Virol 1996;48:8-16.