DİYABETİK AYAKLI BİR HASTAYA AİT YARA ÖRNEĞİNDEN FUSARIUM SPOROTRICHIOIDES İZOLASYONU VE TANIMLANMASI

Yıl 2008, Cilt: 21 Sayı: 1, 68 - 72, 24.06.2015

Mustafa Özyurt Nurittin Ardıç Kadir Turan Şenol Yıldız Oğuz Özyaral Uğur Demirpek Tuncer Haznedaroğlu Türkan Yurdun

Öz

ÖZET

Fusarium türleri bağışıklıkları baskılanmış hastalar için önemli fırsatçı patojenlerdir. Bu çalışmada, diyabetik ayaklı bir hastadan izole edilen patojenin, konvansiyonel ve moleküler tekniklerle tanımlanması yapılarak, Fusarium sporotrichioides olduğu sonucuna varıldı. HPLC analizleri, patojenin T-2 toksinini üretmediği görüldü. Bu nedenle, laboratuvar tanılarında, Fusarium’lar sadece çevresel kontaminantlar olarak değerlendirilmemelidir.

Anahtar Kelimeler: Fusarium, Diyabetik ayak, Fırsatçı patojen

Anahtar Kelimeler

Fusarium, Diyabetik ayak, Fırsatçı patojen

Kaynakça

• 1. Brown DW, McCormick SP, Alexander NJ, Proctor RH, Desjardins AE. Inactivation of a cytochrome P- 450 is a determinant of trichotecene diversity in Fusarium species. Fungal Genet Biol 2002; 36: 224- 233.
• 2. Kenedy MJ, Sigler L. Aspergillus, Fusarium, and other opportunistic moniliaceous fungi. In: Murray P, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds., Manuel of Clinical Microbiology, 6th ed. Washington D C: ASM Press, 1995; 765-790.
• 3. Cenis JL. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 1992 May.11; 20 (9): 2380.
• 4. Wilson A, Simpson D, Chandler E, Jennings P, Nicholson P. Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae. FEMS Microbiol Lett 2004; 233: 69-76.
• 5. Jimenez M, Mateo JJ, Mateo R. Determination of typeA trichothecenes by determination of typeA trichothecenes by high-performance liquid chromatography with coumarin-3-carbonyl chloride derivatisation and fluorescence detection. J Chromatogr 2000; A 870: 473-481.
• 6. Jimenez M, Mateo R. Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high-performance liquid chromatography. J Chromatogr 1997; 778: 363- 372.
• 7. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky JJ, White TJ, eds., PCR Protocols, San Diego, Academic Press, 1990:315-322.
• 8. Waalwijk C, De Koning JRA, Baayen RP, Gams W. Discordant groupings of Fusarium spp. from the sections Elegans, Liseola and Dlaminia based on ribosomal ITS1 and ITS2 sequences. Mycologia 1996; 88; 361-368.
• 9. Samson RA, Hoekstra ES Oorschot CA. Introduction to food-borne fungi. 2nd ed.,78-95, CBS, The Nederland. 1984.
• 10. Mateo JJ, Mateo R, Jimenez M. Accumulation of type A trichothecenes in maize, wheat and rice by F. sporotrichioides isolates under diverse culture conditions. Int J Food Microbiol 2002; 72: 115-123.
• 11. Bigley VH, Duarte RF, Gosling RD, Kibbler CC, Seaton S, Potter M. Fusarium dimerum infection in a stem cell transplant recipient treated successfully with voriconazole. Bone Marrow Transpl 2004; 34: 815- 817.
• 12. Moschovi M, Trimis G, Anastasopoulos J, Kanariou M, Raftopoulou A, Tzortzatou-Stathopoulou F. Subacute vertebral osteomyelitis in a child with diabetes mellitus associated with Fusarium. Pediatr Int 2004; 46: 740-742.
• 13. Hemashettar BM, Siddaramappa B, Padhye AA, Sigler L, Chandler FW. Wite grain mycetoma caused by a cylindrocarpon sp. in India. J Clin Microbiol 2000; 38: 4288-4291.
• 14. Cocuroccia B, Gaido J, Gubinelle E, Annessi G, Girolomoni G. Localized cutaneous hyalohyphomycosis caused by a Fusarium species infection in a renal transplant patient. J Clin Microbiol 2003; 41: 905-907.