2-MERCAPTOETAN SÜLFONAT (MESNA)’IN, PROTAMİN SÜLFATIN TETİKLEDİĞİ MESANE HASARI ÜZERİNDEKİ KORUYUCU ETKİLERİ

Year 2005, Volume: 18 Issue: 1, 6 - 12, 29.06.2015

Beyhan Sağlam Esra Cikler Ali Zeybek Şule Çetinel Feriha Ercan Göksel Şener

Abstract

Amaç: Bu morfolojik ve biyokimyasal çalışmanın amacı 2-mercaptoetan sulfonat (MESNA)’ın protamin sülfat (PS)’ ın tetiklediği
mesane hasarı üzerindeki koruyucu etkilerinin araştırılmasıdır.
Gereç ve Yöntem: Wistar albino dişi sıçanlar kateterize edilerek mesane içine fosfat tamponu (kontrol grubu) ya da fosfat tamponu
içinde çözünmüş PS (PS grubu) infüze edildi. PS + MESNA grubundaki sıçanlara PS verilmesinden sonra, 3 gün boyunca, 50 mg/kg
MESNA i.p. olarak enjekte edildi. Mesane morfolojleri ışık ve taramalı elektron mikroskopi düzeylerinde incelendi; ayrıca dokudaki
malondialdehit ve glutatyon düzeylerine de bakıldı.
Bulgular: PS grubunda ülserli alanlar, düzensiz mukus tabakası, inflamatuvar hücre infiltrasyonu ve mast hücre sayısında artış
gözlendi. PS + MESNA grubunda PS grubuna oranla, normal ürotel görüntüsü ve glikozaminoglikan tabakası ile inflamatuvar
hücrelerin ve mukozal mast hücrelerinin sayısında azalma izlendi. PS’ nin tetiklemesi ile oluşan hasarda önemli rol oynayabilecek
olan serbest radikallerin ortaya çıkması sonucunda malondialdehit düzeyi artmış glutatyon düzeyi ise düşmüş bulundu. PS + MESNA
grubunda morfolojik bulgularla uyumlu olarak, malondialdehit düzeyi önemli derecede düşmüş, glutatyon düzeyi ise artmıştır.
Sonuç: Bu bulgular, mesane mukozasında PS’ ın tetiklediği morfolojik hasar ve biyokimyasal değişikliklerin MESNA tedavisi ile
önemli düzeyde önlenebileceğini göstermektedir.

Keywords

MESNA, Protamin sülfat, Mesane

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