Yıl 2005,
Cilt: 18 Sayı: 2, 76 - 80, 29.06.2015
Öz
-
Anahtar Kelimeler
Kaynakça
- 1. Cox NH, More IA, McCruden D, et al. Electron microscopy of clinically normal skin of diabetic patients. Clin Exper Dermatol 1988; 13:11-15.
- 2. Monnier VM, Bautista O, Kenny D, et al. Skin collagen glycation, glycooxidation and cross linking are lower in subjects with long term intensive versus conventional therapy of type 1 diabetes. Diabetes 1999;48: 870-880.
- 3. Okano Y, Masaki H, Sakurai H. Dysfunction of dermal fibroblasts induced by advanced glycation end products (AGEs) and contribution of a nonspecific interaction with cell membrane and AGEs. J Dermatol Sci 2002;29: 171-180.
- 4. Beisswenger P, Makita Z, Curphey TJ, et al. Formation of immunochemical advanced glycosylation end products precedes and correlates with early manifestations of renal and retinal disease in diabetes. Diabetes 1995;44:824-829.
- 5. Kofoed JA, Bozzini CE, Alippi RM .Skin acid glycoaminoglycans in alloxan diabetic rats. Diabetes 1970; 19:732-733.
- 6. Sternberg M, Cohen-Forterre L, Peyroux J. Connective tissue in diabetes mellitus: Biochemical alterations of the intercellular matrix with special reference to proteolycans, collagens and basement membranes. Diabete Metab 1985; 11:27-50.
- 7. Bannai C, Yamazaki M, Matsushima Y, et al. Amelioration of dermal lesions in streptozotocininduced diabetic rats by aminoguanidine. Diabetes Res 1992; 20:87-95.
- 8. Kupehella CE, Matsuoko LY, Bryan B, et al. Histochemical evaluation of glycosaminoglycan deposition in the skin. J Histochem Cytochem 1984; 32:1121-1124.
- 9. O'Brien TE, Metheney CD, Polansky JR Immunofluorescence method for quantifying the trabecular meshwork glucocorticoid response (TIGR) protein in trabecular meshwork and Schlemm's canal cells. Curr Eye Res 1999;19:517-524.
- 10. Buckingham BA, Uitto J, Sandborg C, et al. Scleroderma-like changes in insulin dependent diabetes mellitus: clinical and biochemical studies. Diabetes Care 1984; 7:163-169.
- 11. Schneir M, Ramamurthy N, Galub L. Skin collagen metabolism in the streptozotocin-induced diabetic rat. Enhanced catabolism of collagen formed both before and during diabetic state. Diabetes 1982; 31:426-431.
DİYABETİK SIÇAN MODELİNDE AMİNOGUANİDİN TEDAVİSİNİN EKSTRASELÜLER MATRİKS PROTEİN EKSPRESYONU VE KOLLOJEN YAPISINA ETKİSİ
Öz
Amaç: Diyabete bağlı cilt değişiklikleri klinik olarak normal durumdayken başlamaktadır ve metabolik kontrolle doğru orantılıdır. Bu çalışmanın amacı diyabetik sıçan modelinde aminoguanidin tedavisinin ekstraselüler matriks protein ekspresyonu ve kollojen yapısına etkisini incelemektir.
Yöntem: Bu amaçla streptozosin ile diyabet oluşturulmuş 12 sıçandan 6 tanesine içme suyuna aminoguanidin eklenerek, diğerleri ise ilaçsız olarak 8 hafta boyunca izlendi. Altı sıçan da sağlıklı kontrol olarak takip edildi. Takip süresi sonunda cilt dokuları alınarak incelendi.
Bulgular: Işık mikroskopisinde diyabetik sıçanlarda bazal membranda kalınlaşma, dermal ödem, vasküler membran kalınlaşması, endotel proliferasyonu ve perivasküler dejenerasyon gözlendi. İmmünhistokimyasl boyamada kollojen IV ve TGF-β’da azalma görüldü. Tüm bu değişikliklerin aminoguanidin tedavisi ile oluşmadığı izlendi.
Sonuç: Bu çalışma diyabetin klinik olarak normal görülen ciltte mikroskopik bozukluklar oluşturduğunu doğrulamaktadır. Aminoguanidin tedavisi cilt değişikliklerinin gelişimini engellemektedir.
Anahtar Kelimeler: Diabetes Mellitus, Cilt, Aminoguanidin
Anahtar Kelimeler
Kaynakça
- 1. Cox NH, More IA, McCruden D, et al. Electron microscopy of clinically normal skin of diabetic patients. Clin Exper Dermatol 1988; 13:11-15.
- 2. Monnier VM, Bautista O, Kenny D, et al. Skin collagen glycation, glycooxidation and cross linking are lower in subjects with long term intensive versus conventional therapy of type 1 diabetes. Diabetes 1999;48: 870-880.
- 3. Okano Y, Masaki H, Sakurai H. Dysfunction of dermal fibroblasts induced by advanced glycation end products (AGEs) and contribution of a nonspecific interaction with cell membrane and AGEs. J Dermatol Sci 2002;29: 171-180.
- 4. Beisswenger P, Makita Z, Curphey TJ, et al. Formation of immunochemical advanced glycosylation end products precedes and correlates with early manifestations of renal and retinal disease in diabetes. Diabetes 1995;44:824-829.
- 5. Kofoed JA, Bozzini CE, Alippi RM .Skin acid glycoaminoglycans in alloxan diabetic rats. Diabetes 1970; 19:732-733.
- 6. Sternberg M, Cohen-Forterre L, Peyroux J. Connective tissue in diabetes mellitus: Biochemical alterations of the intercellular matrix with special reference to proteolycans, collagens and basement membranes. Diabete Metab 1985; 11:27-50.
- 7. Bannai C, Yamazaki M, Matsushima Y, et al. Amelioration of dermal lesions in streptozotocininduced diabetic rats by aminoguanidine. Diabetes Res 1992; 20:87-95.
- 8. Kupehella CE, Matsuoko LY, Bryan B, et al. Histochemical evaluation of glycosaminoglycan deposition in the skin. J Histochem Cytochem 1984; 32:1121-1124.
- 9. O'Brien TE, Metheney CD, Polansky JR Immunofluorescence method for quantifying the trabecular meshwork glucocorticoid response (TIGR) protein in trabecular meshwork and Schlemm's canal cells. Curr Eye Res 1999;19:517-524.
- 10. Buckingham BA, Uitto J, Sandborg C, et al. Scleroderma-like changes in insulin dependent diabetes mellitus: clinical and biochemical studies. Diabetes Care 1984; 7:163-169.
- 11. Schneir M, Ramamurthy N, Galub L. Skin collagen metabolism in the streptozotocin-induced diabetic rat. Enhanced catabolism of collagen formed both before and during diabetic state. Diabetes 1982; 31:426-431.
Toplam 11 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 29 Haziran 2015 |
| Yayımlandığı Sayı | Yıl 2005 Cilt: 18 Sayı: 2 |